Cryopreservation (−20 °C) of canine corneoscleral tissue: histological, microbiological, and ultrastructural study

Costa, Daniel; Leiva, Marta; Naranjo, Carolina; Ríos, José; Peña, María

doi:10.1111/vop.12544

Cited by 5 publications

(4 citation statements)

References 44 publications

(68 reference statements)

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“…The procedure of sample preparation for TEM observation was followed as described by Costa [ 26 ]. Leaf samples of wild type and yl mutant were prepared for TEM from the 1 st expanded true leaf when the second leaf expanded fully.…”

Section: Methodsmentioning

confidence: 99%

Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)

et al. 2022

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Background Leaf color mutants are ideal materials to study pigment metabolism and photosynthesis. Leaf color variations are mainly affected by chlorophylls (Chls) and carotenoid contents and chloroplast development in higher plants. However, the regulation of chlorophyll metabolism remains poorly understood in many plant species. The chloroplast signal-recognition particle system is responsible for the insertion of the light-harvesting chlorophyll a/b proteins (LHCPs) to thylakoid membranes, which controls the chloroplast development as well as the regulation of Chls biosynthesis post-translationally in higher plants. Results In this study, the yellow leaf cucumber mutant, named yl, was found in an EMS-induced mutant library, which exhibited a significantly reduced chlorophyll content, abnormal chloroplast ultrastructure and decreased photosynthetic capacity. Genetic analysis demonstrated that the phenotype of yl was controlled by a recessive nuclear gene. Using BSA-seq technology combined with the map-based cloning method, we narrowed the locus to a 100 kb interval in chromosome 3. Linkage analysis and allelism test validated the candidate SNP residing in CsaV3_3G009150 encoding one homolog of chloroplast signal-recognition particle (cpSRP) receptor in Arabidopsis, cpFtsY, could be responsible for the yellow leaf phenotype of yl. The relative expression of CscpFtsY was significantly down-regulated in different organs except for the stem, of yl compared with that in the wild type (WT). Subcellular localization result showed that CscpFtsY located in the chloroplasts of mesophyll cells. Conclusions The yl mutant displayed Chls-deficient, impaired chloroplast ultrastructure with intermittent grana stacks and significantly decreased photosynthetic capacity. The isolation of CscpFtsY in cucumber could accelerate the progress on chloroplast development by cpSRP-dependant LHCP delivery system and regulation of Chls biosynthesis in a post-translational way.

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Section: Methodsmentioning

confidence: 99%

Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)

et al. 2022

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“…Chls extraction and quanti cation was done following the published protocol [23]. Leaf samples for TEM investigation were made as the method reported by Costa [24] with some modi cations.…”

Section: Phenotype Morphological Characterization and Tem Observationmentioning

confidence: 99%

Fine Mapping of CscpFtsY, a Gene Conferring the Yellow Leaf Phenotype in Cucumber (Cucumis sativus L.)

Zha

Yin

Cheng

et al. 2022

Preprint

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Background Leaf color mutants are ideal materials to study pigment metabolism and photosynthesis. Leaf color variations are mainly affected by chlorophylls (Chls) and carotenoid contents and chloroplast development in higher plants. However, the regulation of chlorophyll metabolism remains poorly understood in many plant species. The chloroplast signal-recognition particle system is responsible for the insertion of the light harvesting chlorophyll a/b proteins (LHCPs) to thylakoid membranes, which controls the chloroplast development as well as the regulation of Chls biosynthesis posttranslationally in higher plants. Results In this study, the yellow leaf cucumber mutant, named yl, was found in an EMS-induced mutant library, which exhibited a significantly reduced chlorophyll content, abnormal chloroplast ultrastructure and decreased photosynthetic capacity. Genetic analysis demonstrated that the phenotype of yl was controlled by a recessive nuclear gene. Using BSA-seq technology combined with the map-based cloning method, we narrowed the locus to a 100 kb interval in chromosome 3. Linkage analysis and allelism test validated the candidate SNP residing in CsaV3_3G009150 encoding one homolog of chloroplast signal-recognition particle (cpSRP) receptor in Arabidopsis, cpFtsY, could be responsible for the yellow leaf phenotype of yl. The relative expression of CscpFtsY was significantly down-regulated in different organs except for the stem, of yl compared with that in the wild type (WT). Subcellular localization result showed that CscpFtsY located in chloroplasts of mesophyll cells. Conclusions The yl mutant displayed Chls-deficient, impaired chloroplast ultrastructure with intermittent grana stacks and significantly decreased photosynthetic capacity. The isolation of CscpFtsY in cucumber could accelerate the progress on chloroplast development by cpSRP-dependant LHCP delivery system and regulation of Chls biosynthesis in a post-translational way.

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“…Firstly, the graft preparation is associated with a number of legal and physical issues. Secondly, the preservation of biomechanical and optical functions of the graft and its storage are also problems of high complexity (Costa, Leiva, & Gimenez, 2018;Ito, Terakado, Ichikawa, Zama, Minami, Kudo, & Kanemaki, 2014;Lacerda, Gimenez, Laguna, & Costa, 2017;Maggs, Miller, & Ofri, 2008;Alkan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…In world practice, the cryopreserved corneas are defrosted just before application, which facilitates their storage. However, deep freezing damages the endothelial layer, which results in corneal opacity after surgery (Costa, Leiva, & Gimenez, 2018).…”

Section: Introductionmentioning

confidence: 99%

Penetrating Keratoplasty with a Preserved Donor Cornea in Veterinary Ophthalmology

Usha¹,

Kontsevaya²,

Lutsay³

et al. 2019

JMBR

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The present article describes the penetrating keratoplasty using the technique of rehydration of donor cornea dehydrated over silica gel, as well as complications that can arise. The indications included corneal ulcers, descemetocoele, corneal sequestrum and corneal perforation. 37 (88.37%) out of 43 corneal transplants had a favorable outcome in surgeries with complete excision of abnormal tissues. In 5 cases (11.63%), the authors faced such complications as suture failure, formation of anterior synechias, incomplete epithelization after the suture removal, and transplant swelling reversed with the preservation of eye optical functions, and secondary glaucoma in the late postoperative period that ended up in eyeball endoprosthesis transplantation. Due to a high rate of efficiency of the proposed method, penetrating keratoplasty with rehydrated grafts can be recommended for the treatment in case of medical emergency experienced by cats and dogs.

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Cryopreservation (−20 °C) of canine corneoscleral tissue: histological, microbiological, and ultrastructural study

Cited by 5 publications

References 44 publications

Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)

Fine mapping of CscpFtsY, a gene conferring the yellow leaf phenotype in cucumber (Cucumis sativus L.)

Fine Mapping of CscpFtsY, a Gene Conferring the Yellow Leaf Phenotype in Cucumber (Cucumis sativus L.)

Penetrating Keratoplasty with a Preserved Donor Cornea in Veterinary Ophthalmology

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