Cryopreservable arrays of paper-based 3D tumor models for high throughput drug screening

Samara, Bisan; Deliorman, Muhammedin; Sukumar, Pavithra; Qasaimeh, Mohammad A.

doi:10.1039/d0lc01300e

Cited by 15 publications

(14 citation statements)

References 36 publications

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“…The gray intensity of circle-shaped paper-based units in Figure e showed a coefficient of variation of less than 3% (Figure f). The above results indicated that the paper-based array might be used for the high-throughput formation of homogeneous droplets, with the potential for high-throughput drug screening …”

Section: Resultsmentioning

confidence: 79%

“…The above results indicated that the paper-based array might be used for the high-throughput formation of homogeneous droplets, with the potential for high-throughput drug screening. 65 One big advantage of the bottom-up method is that paperbased patterns can be fabricated on various substrates for versatile applications. As shown in Figure 3g, 1 mm × 20 mm paper-based lines were printed on the low-cost and widely available PET film.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Rapid Assembly of Cellulose Microfibers into Translucent and Flexible Microfluidic Paper-Based Analytical Devices via Wettability Patterning

Wang

et al. 2022

Anal. Chem.

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Microfluidic paper-based analytical devices (μPADs) are emerging as powerful analytical platforms in clinical diagnostics, food safety, and environmental protection because of their low cost and favorable substrate properties for biosensing. However, the existing top-down fabrication methods of paper-based chips suffer from low resolution (>200 μm). Additionally, papers have limitations in their physical properties (e.g., thickness, transmittance, and mechanical flexibility). Here, we demonstrate a bottom-up approach for the rapid fabrication of heterogeneously controlled paper-based chip arrays. We simply print a wax-patterned microchip with wettability contrasts, enabling automatic and selective assembly of cellulose microfibers to construct predefined paper-based microchip arrays with controllable thickness. This paper-based microchip printing technology is feasible for various substrate materials ranging from inorganic glass to organic polymers, providing a versatile platform for the full range of applications including transparent devices and flexible health monitoring. Our bottom-up printing technology using cellulose microfibers as the starting material provides a lateral resolution down to 42 ± 3 μm and achieves the narrowest channel barrier down to 33 ± 2 μm. As a proof-of-concept demonstration, a flexible paper-based glucose monitor is built for human health care, requiring only 0.3 μL of sample for testing.

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Section: Resultsmentioning

confidence: 79%

Section: ■ Results and Discussionmentioning

confidence: 99%

Rapid Assembly of Cellulose Microfibers into Translucent and Flexible Microfluidic Paper-Based Analytical Devices via Wettability Patterning

Wang

et al. 2022

Anal. Chem.

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“…Therefore, data mining can help us find valuable information from massive amounts of data and convert it into meaningful knowledge. At present, life science research is transforming from experimental analysis combined with data accumulation to data analysis to guide experimental verification [ 42 ]. Network pharmacology and bioinformatics analysis can be based on massive experimental data and clinical trial results, contributing to exploring the potential mechanism of TCM treatment and prevention of diseases, especially for the research on the complex chemical components of Chinese herbal medicine and the multi-target and multi-mechanism of diseases [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Study on the potential active components and molecular mechanism of Xiao Huoluo Pills in the treatment of cartilage degeneration of knee osteoarthritis based on bioinformatics analysis and molecular docking technology

Chen

Lin

et al. 2021

J Orthop Surg Res

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Background Knee osteoarthritis is a common joint degenerative disease. Xiao Huoluo Pills (XHLP) has been used to treat degenerative diseases such as osteoarthritis and hyperosteogeny. However, XHLP’s specific effective ingredients and mechanism of action against osteoarthritis have not been explored. Therefore, bioinformatics technology and molecular docking technology are employed in this study to explore the molecular basis and mechanism of XHLP in the treatment of knee osteoarthritis. Methods Public databases (TCMSP, Batman-TCM, HERB, DrugBank, and UniProt) are used to find the effective active components and corresponding target proteins of XHLP (screening conditions: OB > 30%, DL ≥ 0.18). Differentially expressed genes related to cartilage lesions of knee osteoarthritis are obtained based on the GEO database (screening conditions: adjust P value < 0.01, |log2 FC|≥1.0). The Venn package in R language and the BisoGenet plug-in in Cytoscape are adopted to predict the potential molecules of XHLP in the treatment of knee osteoarthritis. The XHLP-active component-target interaction network and the XHLP-knee osteoarthritis-target protein core network are constructed using Cytoscape software. Besides, GO/KEGG enrichment analysis on core genes is performed using the Bioconductor package and clusterProfiler package in the R language to explain the biological functions and signal pathways of the core proteins. Finally, molecular docking is performed through software such as Vina, LeDock, Discovery Studio 2016, PyMOL, AutoDockTools 1.5.6, so as to verify the binding ability between the active components of the drug and the core target protein. Results XHLP has been screened out of 71 potentially effective active compounds for the treatment of OA, mainly including quercetin, Stigmasterol, beta-sitosterol, Izoteolin, and ellagic acid. Knee osteoarthritis cartilage lesion sequencing data (GSE114007) was screened out of 1672 differentially expressed genes, including 913 upregulated genes and 759 downregulated genes, displayed as heat maps and volcano maps. Besides, 33 core target proteins are calculated by Venn data package in R and BisoGenet plug-in in Cytoscape. The enrichment analysis on these target genes revealed that the core target genes are mainly involved in biological processes such as response to oxygen levels, mechanical stimulus, vitamin, drug, and regulation of smooth muscle cell proliferation. These core target genes are involved in signaling pathways related to cartilage degeneration of knee osteoarthritis such as TNF signaling pathway and PI3K-Akt signaling pathway. Finally, the molecular docking verification demonstrates that some active components of the drug have good molecular docking and binding ability with the core target protein, further confirming that XHLP has the effect of inhibiting cartilage degeneration in knee osteoarthritis. Conclusions In this study, based on the research foundation of bioinformatics and molecular docking technology, the active components and core target molecules of XHLP for the treatment of cartilage degeneration of knee osteoarthritis are screened out, and the potential mechanism of XHLP inhibiting cartilage degeneration of knee osteoarthritis is deeply explored. The results provide theoretical basis and new treatment plan for XHLP in the treatment of knee osteoarthritis.

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“…Optical and electric signals can be read-out by portable devices like smartphone and electric watch [43,44]. Therefore, combined with multiple detection technologies based on diverse sensing materials and portable signal readers, the POCT was realized on μPads and applied in the fields of disease analysis [45][46][47][48] (biological fluids like whole blood, serum, sweat, tears, urine, saliva, cells, viruses), environmental monitoring [49,50] (water, gas, soil) and food control [51].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (μPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with μPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on μPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of μPads for POCT were discussed.

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Cryopreservable arrays of paper-based 3D tumor models for high throughput drug screening

Abstract: Schematic representation of the methodology developed for creating cryopreservable high throughput paper-based arrays of 3D tumor models for drug screening applications.

Cited by 15 publications

References 36 publications

Rapid Assembly of Cellulose Microfibers into Translucent and Flexible Microfluidic Paper-Based Analytical Devices via Wettability Patterning

Rapid Assembly of Cellulose Microfibers into Translucent and Flexible Microfluidic Paper-Based Analytical Devices via Wettability Patterning

Study on the potential active components and molecular mechanism of Xiao Huoluo Pills in the treatment of cartilage degeneration of knee osteoarthritis based on bioinformatics analysis and molecular docking technology

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

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