Cryoinjury in endothelial cell monolayers

Ebertz, S.L.; McGann, Locksley E.

doi:10.1016/j.cryobiol.2004.04.003

Cited by 25 publications

(13 citation statements)

References 16 publications

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“…This deformation of cells was caused by the propagation of extracellular ice and mechanical stress. More severely, the cells may detached from the surface as reported by Ebertz et al [10] . We also quantified the detachment of OBs in the freezing process [7] .…”

Section: Extracellular Ice Formationmentioning

confidence: 77%

“…The pictures in Figure 6 clearly showed the broken actin filament of OBs attached to coverslips. Ebertz et al [10] reported that more endothelial cells detached from coverslips when frozen to −196℃ compared with −40℃. This was because that the cells frozen to −196℃ suffered greater tensions (5×) than the cells frozen to −40℃, as calculated using the above values.…”

Section: Differential Thermal Contractionmentioning

confidence: 98%

“…The viability of rabbit corneal keratocytes cells decreased when attached to a surface compared with cells in suspension at the same cooling rate [9] . Ebertz et al [10] found excessive detachment and loss of membrane integrity in endothelial monolayers when frozen to −196℃. It is believed that attached cells are often more susceptible to cryoinjury than cells frozen in suspension [9] .…”

mentioning

confidence: 98%

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Freezing osteoblast cells attached to hydroxyapatite discs and glass coverslips: Mechanisms of damage

Liu

McGrath

2007

SCI CHINA SER E

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Damage mechanisms for osteoblast cells (OBs) attached to hydroxyapatite (HA) discs and glass coverslips were comprehensively investigated. Cell-cell, cell-matrix interaction altered the cryobiological properties of cells. Attached cells were subject to more severe mechanical damage than isolated cells because attached cells had larger contacting area with ice and the three dimensional movements of isolated cells made them more flexible than attached cells that could only deform in one dimension. Results showed that the viability of attached OB cells decreasedsignificantly compared with the viability of isolated OB cells under the same cryopreservation procedure. Extracellular ice, differential thermal contraction, and mechanical stresses were the major damaging factors for OB cells attached to HA discs and glass coverslips.freezing, monolayers, attachment, differential thermal contraction, mechanical stress Damage to isolated cells has been attributed to the two-factor hypothesis [1] . At slow cooling rates, osmotic injury due to solute effect causes damage. While at rapid cooling rates, intracellular ice formation (IIF) is an inherently lethal event. Because several unique modes of damage arise [2] , such as the three dimensional structure of tissues and heat/mass transfer problems [3] , the enhanced cell damage occurs during the cryopreservation of tissues. Therefore, successful cryopreservation methods for isolated cells may not be optimal for attached cells in tissues because the cell-cell and cell-matrix interaction will alter the cryobiological properties of cells [4,5] . We obtained high recovery (about 80%-90%) when freezing osteoblast cells in suspension by two-step method with 10% Me 2 SO [6] , but markedly lower viability was found for the same type of cells attached to hydroxyapatite (HA) discs or glass coverslips [7] . Porsche et al. [8] reported that the re-

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Section: Extracellular Ice Formationmentioning

confidence: 77%

Section: Differential Thermal Contractionmentioning

confidence: 98%

mentioning

confidence: 98%

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Freezing osteoblast cells attached to hydroxyapatite discs and glass coverslips: Mechanisms of damage

Liu

McGrath

2007

SCI CHINA SER E

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“…Thus, the dilute and ideal nonisothermal 2-P model is applied to predict the volumetric change of ICR mouse spermatozoa when cooled at 5°C 3 min À1 in a glycerolÀNaClÀwater mixture according to available data on their membrane permeabilities. 36 The nondilute and nonideal nonisothermal 2-P model, on the other hand, is used to calculate the content of water and CPAs in human corneal keratocytes 37,38 in DMSOÀNaClÀwater and PGÀNaClÀwater mixtures, respectively, during the freezing protocol at 5°C 3 min À1 .…”

Section: Cryobiologicalmentioning

confidence: 99%

“…Applications of the Nondilute and Nonideal Nonisothermal 2-P Model. The characteristics of membrane water, DMSO, and PG permeabilities of human corneal keratocytes were determined by Ebertz et al 37,38 using the KÀK model. L p , P s and σ in the KÀK model are converted into k w and k CPA in eqs 26 and 27, as presented in Tables 5 and 6.…”

Section: Cryobiologicalmentioning

confidence: 99%

Kinetics of Coupling Water and Cryoprotectant Transport across Cell Membranes and Applications to Cryopreservation

Weng

Chen

et al. 2011

J. Phys. Chem. B

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Thermodynamic and kinetic models can provide a wealth of information on the physical response of living cells and tissues experiencing cryopreservation procedures. Both isothermal and nonisothermal models have been proposed so far, accompanied by experimental verification and cryoapplications. But the cryoprotective solution is usually assumed to be dilute and ideal in the models proposed in the literature. Additionally, few nonisothermal models are able to couple the transmembrane transport of water and cryoprotectant during cooling and warming of cells. To overcome these limitations, this study develops a whole new set of equations that can quantify the cotransport of water and cryoprotectant across cell membranes in the nondilute and nonideal solution during the freezing and thawing protocols. The new models proposed here can be simplified into ones consistent with the classic models if some specific assumptions are included. For cryobiological practice, they are applied to predict the volumetric change for imprinting control region (ICR) mouse spermatozoa and human corneal keratocytes in the freezing protocol. The new models can determine the intracellular concentration of cryoprotectant more precisely than others by abandoning the assumptions such as dilute and ideal solutions and nonpermeability of membranes to cryoprotectant. Further, the findings in this study will offer new insights into the physical response of cells undergoing cryopreservation.

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Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation

et al. 2021

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Droplet vitrification has emerged as a promising ice‐free cryopreservation approach to provide a supply chain for off‐the‐shelf cell products in cell therapy and regenerative medicine applications. Translation of this approach requires the use of low concentration (i.e., low toxicity) permeable cryoprotectant agents (CPA) and high post cryopreservation viability (>90%), thereby demanding fast cooling and warming rates. Unfortunately, with traditional approaches using convective heat transfer, the droplet volumes that can be successfully vitrified and rewarmed are impractically small (i.e., 180 picoliter) for <2.5 m permeable CPA. Herein, a novel approach to achieve 90–95% viability in micro‐liter size droplets with 2 m permeable CPA, is presented. Droplets with plasmonic gold nanorods (GNRs) are printed onto a cryogenic copper substrate for improved cooling rates via conduction, while plasmonic laser heating yields >400‐fold improvement in warming rates over traditional convective approach. High viability cryopreservation is then demonstrated in a model cell line (human dermal fibroblasts) and an important regenerative medicine cell line (human umbilical cord blood stem cells). This approach opens a new paradigm for cryopreservation and rewarming of dramatically larger volume droplets at lower CPA concentration for cell therapy and other regenerative medicine applications.

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Cryoinjury in endothelial cell monolayers

Cited by 25 publications

References 16 publications

Freezing osteoblast cells attached to hydroxyapatite discs and glass coverslips: Mechanisms of damage

Freezing osteoblast cells attached to hydroxyapatite discs and glass coverslips: Mechanisms of damage

Kinetics of Coupling Water and Cryoprotectant Transport across Cell Membranes and Applications to Cryopreservation

Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation

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