Cryogenic grinding of electrospun poly-ε-caprolactone mesh submerged in liquid media

Knotek, Petr; Pouzar, Miloslav; Buzgo, Matěj; Křížková, Barbora; Vlček, M.; Míčková, Andrea; Plencner, Martin; Návesník, Jakub; Amler, Evžen; Bělina, Petr

doi:10.1016/j.msec.2012.04.012

Cited by 14 publications

(10 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar architecture was observed by Plencner et al . and Knotek et al . The nano/micro structure combines higher pore size between microfibers with the high adhesion capacity of the nanofibrous part .…”

Section: Discussionsupporting

confidence: 82%

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

et al. 2016

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 82%

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

et al. 2016

View full text Add to dashboard Cite

show abstract

“…An air-dried PLGA mesh was used to obtain CPN particles by using grinding mechanism of chattering, chipping, and erosion of material with applied shear forces under liquid nitrogen. Material hardness and temperature of the grinding condition determine the quality of cryo-grinding particles 63,64 . SEM analysis of the CPN particles indicates that the cryogrinding process was successful to convert electrospun nanofibre mesh into intact short fibrous particles at the size of 10–60 µm (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Electrospun nanofibre mesh of PLGA was subjected through the cryogrinding process as previously described 64 with some modification to the obtained ground powder of nanofibres. A cryogenic impact grinder (6770 Freezer Mill, Spex, USA) with a self-contained liquid nitrogen bath (4–5 L) was used.…”

Section: Methodsmentioning

confidence: 99%

Nano-fibre Integrated Microcapsules: A Nano-in-Micro Platform for 3D Cell Culture

Khanal¹,

Bhattarai²,

Sankar³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Nano-in-micro (NIM) system is a promising approach to enhance the performance of devices for a wide range of applications in disease treatment and tissue regeneration. In this study, polymeric nanofibre-integrated alginate (PNA) hydrogel microcapsules were designed using NIM technology. Various ratios of cryo-ground poly (lactide-co-glycolide) (PLGA) nanofibres (CPN) were incorporated into PNA hydrogel microcapsule. Electrostatic encapsulation method was used to incorporate living cells into the PNA microcapsules (~500 µm diameter). Human liver carcinoma cells, HepG2, were encapsulated into the microcapsules and their physio-chemical properties were studied. Morphology, stability, and chemical composition of the PNA microcapsules were analysed by light microscopy, fluorescent microscopy, scanning electron microscopy (SEM), Fourier-Transform Infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). The incorporation of CPN caused no significant changes in the morphology, size, and chemical structure of PNA microcapsules in cell culture media. Among four PNA microcapsule products (PNA-0, PNA-10, PNA-30, and PNA-50 with size 489 ± 31 µm, 480 ± 40 µm, 473 ± 51 µm and 464 ± 35 µm, respectively), PNA-10 showed overall suitability for HepG2 growth with high cellular metabolic activity, indicating that the 3D PNA-10 microcapsule could be suitable to maintain better vitality and liver-specific metabolic functions. Overall, this novel design of PNA microcapsule and the one-step method of cell encapsulation can be a versatile 3D NIM system for spontaneous generation of organoids with in vivo like tissue architectures, and the system can be useful for numerous biomedical applications, especially for liver tissue engineering, cell preservation, and drug toxicity study.

show abstract

“…The prepared nanofibers were disintegrated by a cryogenic grinding process in order to deliver an injectable nanofibrous scaffold. The process of cryogenic grinding was described recently by our group [ 33 ]. However, here we describe a modified approach based on a dry-cryogenic grinding method.…”

Section: Resultsmentioning

confidence: 99%

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

et al. 2020

Self Cite

View full text Add to dashboard Cite

Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol–biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.

show abstract

Cryogenic grinding of electrospun poly-ε-caprolactone mesh submerged in liquid media

Cited by 14 publications

References 44 publications

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

Nanofibrous polycaprolactone scaffolds with adhered platelets stimulate proliferation of skin cells

Nano-fibre Integrated Microcapsules: A Nano-in-Micro Platform for 3D Cell Culture

Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits

Contact Info

Product

Resources

About