Cryogenic changes in proteases and antiprotease activities of buffalo (Bubalus bubalis) and cattle (Bos taurus) semen

Gurupriya, Vijayasaraswathy S.; Divyashree, Bannur C.; Roy, Sudhir C.

doi:10.1016/j.theriogenology.2013.10.010

Cited by 18 publications

(10 citation statements)

References 23 publications

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“…Moreover, the binding of acrosin inhibitor to its receptor on the sperm membrane is modulated, particularly by spermadhesins, which is considered an acceptor molecule and protects the spermatozoa from acrosin actions until fertilization has occurred (Jonáková et al, 1992;Jelínková et al, 2003;Davidová et al, 2009). Acrosin is an enzyme that functions as an acrosomal protease, being of fundamental importance at the time of fertilization, participating in the lysis of the zona pellucida and subsequent penetration of the spermatozoa into the oocyte (Adham et al, 1997;Gurupriya et al, 2014). Thus, the acrosin inhibitor binds to the acrosin present in seminal plasma that contributes to sperm deathand in doing so inhibits the proteolytic effect from occurring prematurely during transit of sperm cells in the male reproductive tract (Sanz et al, 1992;Jonáková, 1994).…”

Section: Discussionmentioning

confidence: 99%

Functional insights into the role of seminal plasma proteins on sperm motility of buffalo

Codognoto

Yamada

Schmith

et al. 2018

Animal Reproduction Science

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The objective of the present study was to describe the proteins from the seminal plasma of buffalo and correlate these proteins with sperm motility. Ejaculates from sixteen Murrah buffalo were used. Semen collection was performed by electroejaculation, and the ejaculate was evaluated by macroscopic (volume) and microscopic analysis (subjective motility and vigor, as well as sperm concentration). After the analysis, the samples were centrifuged (800g for 10 min and 10,000 for 30 min at 4 °C), and the supernatant (seminal plasma) was used to determine total protein concentration by the Bradford method. Based on total protein concentration, an aliquot (50 μg) was taken to conduct protein in-solution digestion for nano-LC-ESI-Q-TOF mass spectrometry analysis. Samples were divided into two groups, minimal (little sperm motility) and greater (typical sperm motility), based on non-hierarchical clustering considering motility and emPAI protein value. The data were analyzed by multivariate statistical analysis using principal component analysis (PCA) and partial analysis of minimum squares discrimination (PLS-DA). Forty-eight proteins were detected in the seminal plasma, and fifteen were common to two groups. There were six proteins that were significantly different between the groups. The main functions of proteins in seminal plasma were catalytic and binding activity. Spermadhesin protein, ribonuclease, 14-3-3 protein zeta/delta and acrosin inhibitor were in greater amounts in seminal plasma from the group with greater sperm motility; prosaposin and peptide YY were in greater amounts in the group with little sperm motility. The proteins detected in the greater motility group were correlated with sperm protection, including protection against oxidative stress, lipid peroxidation, protease inhibition and prevention of premature capacitation and acrosome reaction. In the group with little sperm motility, one of the identified proteins is considered to be an antifertility factor, whereas the function of other identified protein is not definitive. Results from the present study add to the knowledge base about the molecular processes related with sperm motility, and these findings can be used for determining potential markers of semen quality.

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Section: Discussionmentioning

confidence: 99%

Functional insights into the role of seminal plasma proteins on sperm motility of buffalo

Codognoto

Yamada

Schmith

et al. 2018

Animal Reproduction Science

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“…They have serpin like activity. Gurupriya et al (2014) demonstrated some major and minor proteases in buffalo and cattle seminal plasma with majority having metalloproteinase activity.…”

Section: Proteases and Proteases Inhibitors Of Seminal Plasmamentioning

confidence: 95%

“…Presence of some major proteases was detected in cattle bull semen by Ferrer et al (2012). Recently Gurupriya et al (2014) have identified some major and minor proteases in cryopreserved buffalo and cattle semen.…”

Section: Advances In Animal Andmentioning

confidence: 98%

“…In human, proteases and their regulators are reportable to play role in gamete storage, maturation, activation and ejaculate activity (Propping et al, 1978). Recently, numerous proteolytic enzymes and protease inhibitors have conjointly been detected in bovine, buffalo (Gurupriya et al, 2014), turkey and boar (Metayer et al, 2002) ejaculate. The changes within the activities of proteases and their regulators and their characterization are less studied in livestock species.…”

Section: Introductionmentioning

confidence: 99%

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Proteases and Proteases Inhibitors of Semen - A Review

Gurupriya¹

2014

Adv. Anim. Vet. Sci.

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| The ejaculate composition is extremely complicated and variable among livestock species. The seminal proteins and enzymes are vital for ejaculate metabolism, spermatozoa performance, survival, and transport within the feminine reproductive tract. Proteases and proteinase inhibitors are secreted by the accessory sex glands of the male reproductive tract and find mixed with the spermatozoa throughout ejaculation. But an entire understanding of those enzymes and their performance in numerous class species is not obtainable. The performance of the assorted proteinases and protease inhibitors of ejaculate stay a mystery, and only a few studies are conducted concerning the characterization of those enzymes. Throughout this review, we tend to target the current understanding of the key proteases and their inhibitors of ejaculate from various mammalian species.

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“…Furthermore, MMP 2 and MMP 9 have been shown to be expressed in multiple genital organs including the testicular, epididymal fluids and seminal plasma amongst mammals such as ram, boar, stallion, buffaloes and canine [13][14][15][16]. With regards to reproduction, MMP2 and MMP9 have been associated with ovulation [17] and implantation of the embryo [18].…”

Section: Mmp 2 and Mmpmentioning

confidence: 99%

Gelatin Binding Proteins in Reproductive Physiology

Kumar¹,

Tinson²,

Mulligan³

et al. 2016

Indian J Microbiol

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In order to advance the assisted reproductive technologies used in animals and human beings, it is important to accumulate basic informations about underlying molecular mechanisms that shape the biological processes of reproduction. From within seminal plasma, proteins perform a wide variety of distinct functions that regulate major reproductive events such as fertilization. The ability of such proteins to bind and interact with different antagonistic ions and biomolecules such as polysaccharides, lipids, and other proteins present in the male and female reproductive tract define these capabilities. Over the last two decades, extensive work has been undertaken in an attempt to define the role of seminal plasma proteins, of which, Gelatin binding proteins (GBPs) represent a large family. GBPs comprise of known group of Bovine seminal plasma (BSP) protein family, matrix metallo proteinases (MMP 2 and MMP 9) and fibronectin, which have been widely studied. The presence of a type II repeat is a characteristic feature of GBPs, which is similar in structure to the fibronectin type II domain (fn2), which has ability to bind multiple ligands including gelatin, glycosaminoglycans, choline phospholipids, and lipoproteins. Two fn2 domains are present within the BSP protein family, while, three fn2 domains are found in gelatinases (MMP-2 and MMP9), and ELSPBP1 (Epididymosomes Transfer Epididymal Sperm Binding Protein 1) contains four long fn2 domains. For the most part BSP proteins are exclusively expressed in seminal vesicles although mBSPH1, mBSPH2 and hBSPH1 are all expressed in the epididymis. The expression of gelatinases has been demonstrated in several organs and tissues such as the prostate, testis, epididymis, ovary, human placenta, cervix and endometrial wall. This review intends to bring current updates on the role of GBPs in reproductive physiology to light, which may act as basis for future studies on GBPs.

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Cryogenic changes in proteases and antiprotease activities of buffalo (Bubalus bubalis) and cattle (Bos taurus) semen

Cited by 18 publications

References 23 publications

Functional insights into the role of seminal plasma proteins on sperm motility of buffalo

Functional insights into the role of seminal plasma proteins on sperm motility of buffalo

Proteases and Proteases Inhibitors of Semen - A Review

Gelatin Binding Proteins in Reproductive Physiology

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