Cryogenic 3D printing for tissue engineering

Adamkiewicz, M.; Rubinsky, Boris

doi:10.1016/j.cryobiol.2015.10.152

Cited by 51 publications

(42 citation statements)

References 10 publications

(11 reference statements)

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“…The digital file for the selected 3D shape in STL (stereolithography) format was designed using Blender software [22] and printed using MakerBot MakerWare ™ .…”

Section: Using Fdm 3d Printing To Fabricate Solid Dispersionsmentioning

confidence: 99%

An investigation into the use of polymer blends to improve the printability of and regulate drug release from pharmaceutical solid dispersions prepared via fused deposition modeling (FDM) 3D printing

Alhijjaj

Belton

2016

European Journal of Pharmaceutics and Biopharmaceutics

223

134

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FDM 3D printing has been recently attracted increasing research efforts towards the production of personalized solid oral formulations. However, commercially available FDM printers are extremely limited with regards to the materials that can be processed to few types of thermoplastic polymers, which often may not be pharmaceutically approved materials nor ideal for optimizing dosage form performance of poor soluble compounds. This study explored the use of polymer blends as a formulation strategy to overcome this processability issue and to provide adjustable drug release rates from the printed dispersions. Solid dispersions of felodipine, the model drug, were successfully fabricated using FDM 3D printing with polymer blends of PEG, PEO and Tween 80 with either Eudragit E PO or Soluplus. As PVA is one of most widely used polymers in FDM 3D printing, a PVA based solid dispersion was used as a benchmark to compare the polymer blend systems to in terms processability. The polymer blends exhibited excellent printability and were suitable for processing using a commercially available FDM 3D printer. With 10% drug loading, all characterization data indicated that the model drug was molecularly dispersed in the matrices.During in vitro dissolution testing, it was clear that the disintegration behavior of the formulations significantly influenced the rates of drug release. Eudragit EPO based blend dispersions showed bulk disintegration; whereas the Soluplus based blends showed the 'peeling' style disintegration of strip-by-strip. The results indicated that interplay of the miscibility between excipients in the blends, the solubility of the materials in the dissolution media and the degree of fusion between the printed strips during FDM process can be used to manipulate the drug release rate of the dispersions. This brings new insight into the design principles of controlled release formulations using FDM 3D printing.

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“…The digital file for the selected 3D shape in STL (stereolithography) format was designed using Blender software [22] and printed using MakerBot MakerWare ™ .…”

Section: Using Fdm 3d Printing To Fabricate Solid Dispersionsmentioning

confidence: 99%

An investigation into the use of polymer blends to improve the printability of and regulate drug release from pharmaceutical solid dispersions prepared via fused deposition modeling (FDM) 3D printing

Alhijjaj

Belton

2016

European Journal of Pharmaceutics and Biopharmaceutics

223

134

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“…Accuracy and printing resolution difficult to control due to the hydrogel material spreading during printing, before solidification is complete ( Figure 4B). A recent study by Adamkiewicz and coworkers demonstrated that printing hydrogels in liquid nitrogen reduces thermal stresses during printing, allowing for fabrication of scaffolds with more precisely defined dimensions ( Figure 4B) 78 . Printing directly into liquid nitrogen may allow for complex macroporous structures with soft hydrogel materials, but it would limit the ability to print with cells.…”

Section: Current Limitations and Challengesmentioning

confidence: 99%

“…Many of the early studies in tissue bioprinting created custom-built printers 36b or created modifications to commercially available 3D printers 46,78 . Low-cost ommercially available printers mostly limited to printing with ABS (acrylonitrile butadiene styrene) or PLA by heating the material to very high temperatures (200 -240 o C) before extrusion 80 .…”

Section: Current Limitations and Challengesmentioning

confidence: 99%

3D Bioprinting: New Directions in Articular Cartilage Tissue Engineering

O’Connell

Garcia

Amir³

2017

ACS Biomater. Sci. Eng.

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Bioprinting is a growing field with significant potential for developing engineered tissues with compositional and mechanical properties that recapitulates healthy native tissue. Much of the current research in tissue and organ bioprinting has focused on complex tissues that require vascularization. Cartilage tissue engineering has been successful in developing de novo tissues using homogenous scaffolds. However, as research moves towards clinical application, engineered cartilage will need to maintain homogeneous nutrient diffusion in larger scaffolds and integrate with surrounding tissues. Bioprinting techniques have provided promising results to address these challenges in cartilage tissue engineering. The purpose of this review was to evaluate 3D extrusion-based bioprinting research for developing engineered cartilage. Specifically, we reviewed the potential impact of 3D bioprinting on nutrient diffusion in larger scaffolds, development of scaffolds with spatial variation in cell distribution or mechanical properties, and cultivation of more complex tissues using multiple materials. Finally, we discuss current limitations and challenges in using 3D bioprinting for cartilage tissue engineering and regeneration.

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“…It is possible to quickly master the basics of ice-templating and rapidly get started. Finally, we can combine ice-templating with other materials processing and shaping routes such as foaming [38], tape-casting [39], spraying [40], extrusion [41], co-extrusion [42], or additive manufacturing [43,44]. These combinations make it possible to achieve more complex, hierarchical architectures.…”

Section: The Lure Of Ice-templating: What Makes It Unique ?mentioning

confidence: 99%

The lure of ice-templating: Recent trends and opportunities for porous materials

Deville

2018

Scripta Materialia

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Ice-templating is a simple materials processing and shaping route where the growth of crystals from a solvent template the porosity. Although the principles of ice-templating were established a long time ago, it has lured considerable attention over the past 5 years to become a well-established processing route for porous materials of all kinds. In this viewpoint, I summarize the current status and recent trends of ice-templating studies. I then review the unique assets of ice-templating and propose a roadmap of the most promising or pressing questions and opportunities in this domain.

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Cryogenic 3D printing for tissue engineering

Cited by 51 publications

References 10 publications

An investigation into the use of polymer blends to improve the printability of and regulate drug release from pharmaceutical solid dispersions prepared via fused deposition modeling (FDM) 3D printing

An investigation into the use of polymer blends to improve the printability of and regulate drug release from pharmaceutical solid dispersions prepared via fused deposition modeling (FDM) 3D printing

3D Bioprinting: New Directions in Articular Cartilage Tissue Engineering

The lure of ice-templating: Recent trends and opportunities for porous materials

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