Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells

Chakraborty, Saikat; Mahamid, Julia; Baumeister, Wolfgang

doi:10.1016/j.str.2020.05.013

Cited by 35 publications

(70 citation statements)

References 86 publications

(119 reference statements)

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“…Inset: Close up showing putative thin stalk‐like densities connecting the filaments. Panels (a) and (d‐f) reproduced from reference [123]…”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 53%

“…Recently, cryo‐FIB milling and cryo‐ET were harnessed to explore the nanoscale architecture of MTs inside different mammalian cells and at different cell‐cycle stages using the VPP 123 . In interphase HeLa cells, MTs are spatially entangled in a composite cytoskeletal matrix of actin filaments and IFs (Figure 4a).…”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

“…However, MTs were occasionally found in highly bent configurations inside cells 125,126 . In a recent cryo‐ET study, MT curvature was quantitatively analyzed inside interphase and mitotic cells 123 . The apparent persistence length of MTs in interphase HeLa cells is ~2–3 orders of magnitude lower than their in vitro persistence length, consistent with previous reports based on fluorescence microscopy investigations 127,128 .…”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

“…In contrast, spindle and neuronal MTs show long‐arc‐like bending. Apparently, the matrix surrounding the MTs can substantially modulate the curvature 123 …”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

“…In situ cryo‐ET of eukaryotic cells provided insights into MT and actin coordination inside cells 123 . MTs and actin filaments were found to interact primarily through passive entanglements (Figure 4a).…”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

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Three‐dimensional organization of the cytoskeleton: A cryo‐electron tomography perspective

2020

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Traditionally, structures of cytoskeletal components have been studied ex situ, that is, with biochemically purified materials. There are compelling reasons to develop approaches to study them in situ in their native functional context. In recent years, cryo-electron tomography emerged as a powerful method for visualizing the molecular organization of unperturbed cellular landscapes with the potential to attain near-atomic resolution. Here, we review recent works on the cytoskeleton using cryo-electron tomography, demonstrating the power of in situ studies. We also highlight the potential of this method in addressing important questions pertinent to the field of cytoskeletal biomechanics. K E Y W O R D S actin filaments, cryo-electron tomography, in situ architecture, intermediate filaments, microtubules Abbreviations: 3D, three-dimensional; ATP, adenosine triphosphate;

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“…Inset: Close up showing putative thin stalk‐like densities connecting the filaments. Panels (a) and (d‐f) reproduced from reference [123]…”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 53%

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

“…In contrast, spindle and neuronal MTs show long‐arc‐like bending. Apparently, the matrix surrounding the MTs can substantially modulate the curvature 123 …”

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

Section: Nanoscale Architecture Of the Mt Cytoskeletonmentioning

confidence: 99%

See 3 more Smart Citations