Cryoconservation of seeds of rare Russian orchids

Nikishina, T. V.; Vakhrameeva, M. G.; Варлыгина, Т.И.; Pavlov, V. N.; Shirokov, A. I.; Burov, Alexander; Popovich, E. A.; Popov, A. S.

doi:10.1134/s0012496606030033

Cited by 11 publications

(9 citation statements)

References 3 publications

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“…Similar results were reported by Tikhonova [36] for 30 wild-growing plant species: plant growth and development was not adversely affected by cryopreservation. The normal growth of plants from the seeds treated by LN was shown for two Spanish endemic species [35] seven species of Orchidaceae [37,38]. It appeared that LN exposure only or combined treatments did not induce seed physical damage or abnormal seedling development for all species studied.…”

Section: Discussionmentioning

confidence: 99%

Seed Cryopreservation of Some Medicinal Legumes

Kholina

Воронкова

2012

Journal of Botany

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Seed survival after storage in liquid nitrogen (–196°C) was examined in 12 wild medicinal legume species occurred Far East of Russia. Dry seeds of all species survived cryostorage without loss of viability. Initial germinability varied from 3 to 85%. The stimulatory effect of cryogenic temperature on germination, with or without subsequent chemical scarification, was observed in all species studied with deep physical dormancy or heterogeneous levels of hardseededness. Frozen seeds demonstrated higher germination percentages (the percentage of germinated seeds) and germination rates (time for first seed to germinate (T0) and time required (in days) to reach 50% of the final germination percentage (T50)) than the control ones. The anomalous seedlings were not observed after storage of seeds in liquid nitrogen. This study shows that cryostorage may be successfully applied for conservation of native species without detrimental effects on germination and growth.

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Section: Discussionmentioning

confidence: 99%

Seed Cryopreservation of Some Medicinal Legumes

Kholina

Воронкова

2012

Journal of Botany

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“…For example, germination was enhanced for seeds of the terrestrial orchid Orchis morio following drying to 5%-11% moisture content and immersion for 15 minutes in liquid nitrogen, with the germination of a further eight species being maintained (Pritchard, 1984). Similarly, for the tropical terrestrial and epiphytic orchids Angraecum magdalenae, Calanthe vestita, and Trichopilia tortilis, cryopreservation for 1 mo was found to enhance germination (Nikishina et al 2001).…”

Section: Low-temperature Storage and Cryopreservation Of Orchid Seedsmentioning

confidence: 99%

“…For orchids, these offer some advantages in the potential to assess viability more rapidly through bypassing the need for development of suitable germination media and the long incubation periods necessary for germination. However, the use of vital stains such as tetrazolium chloride, fluorescein diacetate (FDA), and Evans blue has been met with varying degrees of success for orchid seeds (Waes and Debergh 1986;Batty et al 2001;Nikishina et al 2001;Wood et al 2003;Wood and Pritchard 2004;Johnson et al 2007; fig. 5).…”

Section: Challenges In Handling Orchid Seedsmentioning

confidence: 99%

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Merritt

Hay

Swarts

et al. 2014

International Journal of Plant Sciences

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Premise of research. Orchids are among the most enigmatic of plant species. Yet the Orchidaceae comprises more species at risk of extinction than any other plant family. The collection and storage of orchid germplasmprincipally seeds and associated mycorrhizal fungi but also protocorm-like bodies using encapsulation and vitrification techniques-allows for secure ex situ conservation. This article reviews the approaches and techniques used for the ex situ conservation of orchid germplasm, with a focus on seed banking and the use of cryopreservation techniques to improve the longevity of germplasm.Pivotal results. It is increasingly apparent that cryopreservation-the storage of germplasm at ultra-low temperatures (e.g., in liquid nitrogen)-is required for the long-term and low-maintenance conservation of all types of orchid germplasm. For orchid seeds, desiccation tolerance is common, but longevity in storage is poor. Cryopreservation of orchid seeds shows promise, but some complexities in low-temperature storage behavior still require explanation and resolution. The application of more advanced cryopreservation techniques, including encapsulation-dehydration and vitrification, is becoming increasingly common. These techniques provide for the simultaneous storage of orchid propagules with their compatible fungus, while for seeds, vitrification techniques show potential for improving tolerance to the stresses of cryopreservation. Conclusions.A renewed focus on describing the low-temperature storage physiology of orchid seeds to more precisely define the relationship between seed water content, storage temperature, and seed survival is required, as is perhaps the wider adoption of the use of cryoprotectants for seeds. This research, coupled with the development of improved methods of seed viability testing, will support the growing work of germplasm banks to protect orchid biodiversity in the face of habitat loss and potential species extinction.

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“…Kodo millet germination was found to be highest in moringa leaf extract, which is 7% greater than the control. Higher germination percentage was a critical factor in determining seed volume and viability [33], which is aided by the presence of active ingredients [34], micronutrients [35], and biologically active compounds such as phenolic compounds, organic acids, proteins, and alkaloids [36] in natural plant leaf extracts. However, Moringa extracts produced the longest root of 9.21 cm whereas mango leaf extract had longest shoot (8.42 cm) in kodo millet as evidenced from findings of [37] and [38].…”

Section: Influence On Germination Shoot Length Root Length and Vigour...mentioning

confidence: 99%

Tree Leaf Extracts as Biostimulants for influencing Germination Attributes and Biochemical Changes in kodo millet (Paspalum scrobiculatum L.)

Sangeetha¹,

Swaminathan²,

Subramanian³

et al. 2022

YMER

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This study was performed at the Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai-625104, India with a primary objective of evaluating the effectiveness of natural botanicals/tree leaf extracts of three horticultural tree species on germination and associated features in kodo millet and, in coordination with bioassay investigations, biochemical analysis of leaf extracts and enzyme activities in kodo millet. Response Index was computed for comparison and the reasons for the effect, if any, was investigated by GCMS-based identification of the chemicals found in the leaf extracts. The findings showed that treating the seeds with mango leaf extract boosted the seedling vigour index in kodo millet. In addition, the higher response on germination and enzyme activities such as dehdrogenase, α Amylase, catalyse and peroxidase in kodo millet were documented in Moringa oleifera leaf extract. Key words: Bioassay, Mango leaf extract, Moringa oleifera, Germination, kodo millet

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Cryoconservation of seeds of rare Russian orchids

Cited by 11 publications

References 3 publications

Seed Cryopreservation of Some Medicinal Legumes

Seed Cryopreservation of Some Medicinal Legumes

Ex situ Conservation and Cryopreservation of Orchid Germplasm

Tree Leaf Extracts as Biostimulants for influencing Germination Attributes and Biochemical Changes in kodo millet (Paspalum scrobiculatum L.)

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