Cryobank of plant genetic resources in Russian Academy of Sciences

Popov, A. S.; Попова, Елена; Nikishina, T. V.; Vysotskaya, O. N.

doi:10.1016/j.ijrefrig.2005.07.011

Cited by 28 publications

(22 citation statements)

References 17 publications

(21 reference statements)

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“…This study shows that at lower concentrations of sucrose in the preculture medium there is an influx of sucrose into the PLBs, but with the increase of sucrose concentration in preculture media (1.25 M sucrose), the accumulation of sucrose into the PLBs was hindered. When the tissues are exposed to extremely high sucrose concentration, the cell will be over dehydrated this will lead to severe plasmolysis and plasmalemma rapture leading to cell death and inhabitation of sucrose accumulation (Popov et al 2006). Desiccation using silica gel was able to extract more water from the PLBs as compared to the laminar air flow cabinet.…”

Section: Discussionmentioning

confidence: 99%

“…When excessively desiccated membranes undergo structural changes and proteins denature (Hoekstra et al 2001). On the contrary, high moisture content in normal cells under cryopreservation can produce intracellular ice crystals during immersion in LN which causes loss of viability and re-growth ability in samples after re-warming (Hoekstra et al 2001;Bian et al 2002;Popov et al 2006). However, Lambardi et al (2000) reported that low levels of crystallisable water within the plant material are important in ensuring high recovery percentages after the cryostorage.…”

Section: Discussionmentioning

confidence: 99%

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Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Khoddamzadeh

Sinniah

Lynch

et al. 2011

Plant Cell Tiss Organ Cult

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Protocorm-like bodies (PLBs) of Phalaenopsis bellina were successfully cryopreserved by the encapsulationdehydration approach. Various stages in obtaining successful cryopreservation using this method were optimized. Encapsulated PLBs precultured in half-strength MS medium supplemented with 0.75 M sucrose for 3 days exhibited the highest viability in terms of 2,3,5-triphenyltetrazoliumchloride (TTC) reduction. The amount of sucrose in the PLBs after incubation in different concentrations of sucrose for different periods of time determined by HPLC. The highest sucrose concentration was 7 mg/g of PLBs for the PLBs treated with 0.75 M sucrose for 3 days as compared to the control which had only 1 mg/g sucrose. After sucrose preculture, the PLBs were subjected to desiccation using one of two methods. Desiccation using silica gel was more efficient in reducing PLBs moisture content. After 6 h of desiccation, PLBs desiccated using laminar air flow had 43.5% moisture content while for those desiccated using silica gel had 32% moisture content. PLBs desiccated to different moisture contents were plunged into LN. After storage in LN the encapsulated PLBs were re-warmed. Two weeks after re-warming PLBs viability was determined by TTC reduction and re-growth assessed. Encapsulated PLBs precultured with 0.75 M sucrose for 3 days followed by desiccated using silica gel for 5 h resulting in a moisture content of 39% lead to the highest post re-warming viability in terms of TTC reduction (46.6% of control PLBs) and 30% re-growth.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Khoddamzadeh

Sinniah

Lynch

et al. 2011

Plant Cell Tiss Organ Cult

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“…In the Russian Academy of Sciences (Moscow), 15 species of rare medicinal plants and crops are conserved in liquid nitrogen through cell strains and seeds of 230 endangered plant species collected collected in the Russian territory are cryoconserved, as well as seeds from 22 rare tropical and Russian orchids (Popov et al, 2006). Both of these countries are more advanced in cryoconservation of native plant germplasm than Brazil, and Brazilian institutions could also benefit from collaborations with their research groups and institutions.…”

Section: A Global Viewmentioning

confidence: 99%

Cryoconservation of plant germplasm native to Brazil

Civatti¹,

Marchi²,

Torres-Silva³

et al. 2014

Afr. J. Biotechnol.

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“…Cryopreservation is tissue dependant and a personalized cryopreservation protocol for each type of plant species and even genotype needs to be established (Engelmann, 2004). Often the most concerning obstacle during cryopreservation is intracellular crystallization of water with associated deleterious effects such as membrane damage (Popov et al, 2006). To avoid this, explants undergo dehydration and vitrification, by making use of cryoprotectant solutions and encapsulation to prevent intercellular crystallization and osmotic stress (Chapman et al, 2008;Sakai et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and cryopreservation of South African bread wheat (Triticum aestivum L.) genotypes

Roux

Botha

Vyver

2016

South African Journal of Botany

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a b s t r a c t Edited by J Van StadenThe present study focused on the development of efficient in vitro regeneration protocols for six southern African bread wheat genotypes (Triticum aestivum L.). The tested wheat genotypes showed variation in their in vitro coefficiency abilities, with efficiencies ranging from 0 to 36.5%. Gamtoos and Tugela genotypes displayed the highest and lowest regeneration efficiencies, respectively on the tested growth media. The data indicated that immature embryos, isolated 12 days post-anthesis, resulted in embryogenic calli formation 4 to 6 days after initiation in the presence of picloram and/or 2,4-dichlorophenoxyacetic acid (2,4-D). Adventitious shoots emerged from 3 to 4 week old calli in the presence of 6-benzylaminopurine (BAP) or zeatin. Maltose as carbon source, MS vitamin mix and the addition of 50 μM silver nitrate also increased the in vitro regeneration abilities of the wheat explant material. Rooting of emerged in vitro plantlets was established on MS or half strength MS without the addition of any phytohormones. Furthermore, a novel cryopreservation protocol was successfully developed by encapsulation/vitrification/dehydration of immature wheat seeds. Immature seeds encapsulated in alginate beads, vitrified in 0.5 M sucrose, desiccated for 72 h, cryoprotected in 80% glycerol and flash frozen in liquid nitrogen resulted in the highest survival rate of immature embryos isolated after cryopreservation and regenerated in vitro. Desiccation of tissue prior to cryopreservation seems to be the singularly most important step to ensure successful preservation of wheat tissue.

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Cryobank of plant genetic resources in Russian Academy of Sciences

Cited by 28 publications

References 17 publications

Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb.f.) christenson by encapsulation-dehydration

Cryoconservation of plant germplasm native to Brazil

Somatic embryogenesis and cryopreservation of South African bread wheat (Triticum aestivum L.) genotypes

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