Cryobacterium soli sp. nov., isolated from forest soil

Gong, Chunjie; Lai, Qiliang; Cai, Hanfeng; Jiang, Yanming; Liao, Hu; Liu, Yunfei; Xue, Dongsheng

doi:10.1099/ijsem.0.003820

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…soli and C . arcticum described as psychrotolerant soil bacteria (Bajerski et al, 2011; Gong et al, 2020). The species affiliation for most of the isolates from genera Arthrobacter , Pseudomonas , Rhodococcus , Salinibacterium , and Polaromonas was not determined due to the low discriminative ability of 16S rRNA gene sequence analysis.…”

Section: Resultsmentioning

confidence: 99%

“…Isolates of Leifsonia genus were identified as L. antarctica, L. rubra, and L. kafniensis-psychrophilic bacteria, originally discovered in sediment and pond in Antarctica, and Kafni glacier of the Himalayas (Pindi et al, 2009;Reddy et al, 2003). Genus Cryobacterium was represented by species C. soli and C. arcticum described as psychrotolerant soil bacteria (Bajerski et al, 2011;Gong et al, 2020). The species affiliation for most of the isolates from genera Arthrobacter, Pseudomonas, Rhodococcus, Salinibacterium, and Polaromonas was not determined due to the low discriminative ability of 16S rRNA gene sequence analysis.…”

Section: Phylogenetic Relationships Of Isolated Green Snow Bacteria By 16s Rrna Gene Sequencingmentioning

confidence: 99%

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Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica

et al. 2020

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Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by families Burkholderiaceae (46.31%), Flavobacteriaceae (22.98%), and Pseudomonadaceae (17.66%). Identification of 45 fast‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phyla Actinobacteria and Proteobacteria, and are represented by the genera Arthrobacter, Cryobacterium, Leifsonia, Salinibacterium, Paeniglutamicibacter, Rhodococcus, Polaromonas, Pseudomonas, and Psychrobacter. Nearly all bacterial isolates exhibited various growth temperatures from 4°C to 25°C, and some isolates were characterized by a high level of enzymatic activity. Phenotyping using Fourier transform infrared (FTIR) spectroscopy revealed a possible accumulation of intracellular polymer polyhydroxyalkanoates (PHA) or lipids in some isolates. The bacteria showed different lipids/PHA and protein profiles. It was shown that lipid/PHA and protein spectral regions are the most discriminative for differentiating the isolates.

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Section: Resultsmentioning

confidence: 99%

Section: Phylogenetic Relationships Of Isolated Green Snow Bacteria By 16s Rrna Gene Sequencingmentioning

confidence: 99%

Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica

et al. 2020

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“…The type species of Cryobacterium psychrophilum is an obligate psychrophilic actinomycete. At the time of writing, the genus Cryobacterium comprises 11 recognized species, C. psychrophilum (Suzuki et al, 1997;Inoue and Komagata, 2006), Cryobacterium psychrotolerans (Zhang et al, 2007), Cryobacterium mesophilum (Dastager et al, 2008), Cryobacterium roopkundense (Reddy et al, 2010), Cryobacterium arcticum (Bajerski et al, 2011), Cryobacterium flavum and Cryobacterium luteum (Liu et al, 2012), Cryobacterium levicorallinum (Liu et al, 2013), Cryobacterium zongtaii (Liu et al, 2018), Cryobacterium soli (Gong et al, 2019), and Cryobacterium melibiosiphilum (Liu et al, 2019b). All type strains of Cryobacterium except C. mesophilum described by Dastager et al (2008) were isolated from cold environments and recognized as psychrophiles with optional growth temperature ranging from 15 to 20 • C and growth occurs between 0 and 25 • C. The strain C. mesophilum grew between 20 and 28 • C, with optimum growth occurring at 25-28 • C (Dastager et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genomic Insights of Cryobacterium Isolated From Ice Core Reveal Genome Dynamics for Adaptation in Glacier

et al. 2020

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Glacier is the dominant cold habitat in terrestrial environments, providing a model ecosystem to explore extremophilic strategies and study early lives on Earth. The dominant form of life in glaciers is bacteria. However, little is known about past evolutionary processes that bacteria underwent during adaptation to the cryosphere and the connection of their genomic traits to environmental stressors. Aiming to test the hypothesis that bacterial genomic content and dynamics are driven by glacial environmental stressors, we compared genomes of 21 psychrophilic Cryobacterium strains, including 14 that we isolated from three Tibetan ice cores, to their mesophilic counterparts from the same family Microbacteriaceae of Actinobacteria. The results show that psychrophilic Cryobacterium underwent more dynamic changes in genome content, and their genomes have a significantly higher number of genes involved in stress response, motility, and chemotaxis than their mesophilic counterparts ( P < 0.05). The phylogenetic birth-and-death model imposed on the phylogenomic tree indicates a vast surge in recent common ancestor of psychrophilic Cryobacterium (gained the greatest number of genes by 1,168) after the division of the mesophilic strain Cryobacterium mesophilum . The expansion in genome content brought in key genes primarily of the categories “cofactors, vitamins, prosthetic groups, pigments,” “monosaccharides metabolism,” and “membrane transport.” The amino acid substitution rates of psychrophilic Cryobacterium strains are two orders of magnitude lower than those in mesophilic strains. However, no significantly higher number of cold shock genes was found in psychrophilic Cryobacterium strains, indicating that multi-copy is not a key factor for cold adaptation in the family Microbacteriaceae, although cold shock genes are indispensable for psychrophiles. Extensive gene acquisition and low amino acid substitution rate might be the strategies of psychrophilic Cryobacterium to resist low temperature, oligotrophy, and high UV radiation on glaciers. The exploration of genome evolution and survival strategies of psychrophilic Cryobacterium deepens our understanding of bacterial cold adaptation.

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“…Other subsequently published descriptions of new Cryobacterium species included the type strain of C. mesophilum in phylogenetic trees but did not include species closely related to C. mesophilum but belonging to other genera. This resulted in a seemingly monophyletic nature of the genus Cryobacterium [63][64][65][66][67][68][69]. The only exception was the description of C. tepidiphilum by Wang et al [70], who reported the phylogenetic nature of the genus and recommended a taxonomic revision.…”

Section: Proposal To Reclassify Cryobacterium Mesophilum Dastager Et Al 2008mentioning

confidence: 99%

“…mesophilum but belonging to other genera. This resulted in a seemingly monophyletic nature of the genus Cryobacterium [63–69]. The only exception was the description of C.…”

Section: Proposal To Reclassify Cryobacterium Mesophilum Dastager Et Al 2008mentioning

confidence: 99%

Aurantimicrobium photophilum sp. nov., a non-photosynthetic bacterium adjusting its metabolism to the diurnal light cycle and reclassification of Cryobacterium mesophilum as Terrimesophilobacter mesophilus gen. nov., comb. nov.

Hahn

Pitt

Koll

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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The aerobic primarily chemoorganotrophic actinobacterial strain MWH-Mo1T was isolated from a freshwater lake and is characterized by small cell lengths of less than 1 µm, small cell volumes of 0.05–0.06 µm3 (ultramicrobacterium), a small genome size of 1.75 Mbp and, at least for an actinobacterium, a low DNA G+C content of 54.6 mol%. Phylogenetic analyses based on concatenated amino acid sequences of 116 housekeeping genes suggested the type strain of Aurantimicrobium minutum affiliated with the family Microbacteriaceae as its closest described relative. Strain MWH-Mo1T shares with the type strain of that species a 16S rRNA gene sequence similarity of 99.6 % but the genomes of the two strains share an average nucleotide identity of only 79.3 %. Strain MWH-Mo1T is in many genomic, phenotypic and chemotaxonomic characteristics quite similar to the type strain of A. minutum . Previous intensive investigations revealed two unusual traits of strain MWH-Mo1T. Although the strain is not known to be phototrophic, the metabolism is adjusted to the diurnal light cycle by up- and down-regulation of genes in light and darkness. This results in faster growth in the presence of light. Additionally, a cell size-independent protection against predation by bacterivorous flagellates, most likely mediated by a proteinaceous cell surface structure, was demonstrated. For the previously intensively investigated aerobic chemoorganotrophic actinobacterial strain MWH-Mo1T (=CCUG 56426T=DSM 107758T), the establishment of the new species Aurantimicrobium photophilum sp. nov. is proposed.

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Cryobacterium soli sp. nov., isolated from forest soil

Cited by 11 publications

References 24 publications

Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica

Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica

Genomic Insights of Cryobacterium Isolated From Ice Core Reveal Genome Dynamics for Adaptation in Glacier

Aurantimicrobium photophilum sp. nov., a non-photosynthetic bacterium adjusting its metabolism to the diurnal light cycle and reclassification of Cryobacterium mesophilum as Terrimesophilobacter mesophilus gen. nov., comb. nov.

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