Cryo-EM elucidates mechanism of action of bacterial pore-forming toxins

Mondal, Anish Kumar; Lata, Kusum; Singh, Mahendra; Chatterjee, Shamaita; Chauhan, Aakanksha; Puravankara, Sindhoora; Chattopadhyay, Kausik

doi:10.1016/j.bbamem.2022.184013

Cited by 6 publications

(2 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among these are (i) a putative Zn-dependent endopeptidases related to the RTX toxin family (1.C.11, e.g. 2 clusters in N. epidermidis) which in Gram-negative bacteria are implicated in virulence 48,49 , (ii) enrichment of further families of secreted peptidases (e.g. peptidase family M23, Subtilisinlike serine protease), that can have diverse roles, both in tissue invasion and establishment but also in modulation of other virulence factors 50 , (iii) extracellular or membrane-attached putative triacylglycerol esterases/lipases (arCOG06923, PF02089) of which the skin strains encode 2-3 orthologs, that in bacterial colonization/infection of host epithelia enable catabolism of host fatty acids in order to alter host immune response or serve anabolic purposes 51,52 .…”

Section: Members Of the Genus Nitrosocosmicus Are Equipped For The Co...mentioning

confidence: 99%

Molecular Tracking and Cultivation Reveal Ammonia Oxidizing Archaea as Integral Members of the Human Skin Microbiome

Mahnert,

Dreer,

Perier

et al. 2024

Preprint

View full text Add to dashboard Cite

Ammonia oxidizing archaea (AOA) have been repeatedly detected in the human skin microbiome through molecular and biochemical analyses, yet their persistence, physiology, and adaptations remain poorly understood. Here, we describe two cultivated strains, Candidatus Nitrosocosmicus epidermidis and Ca. Nitrosocosmicus unguis, enriched from human skin samples. These autotrophic strains grow on ammonia and urea as sole energy sources. Genomic islands and expanded gene families in their genomes testify to the capacity of colonizing skin, including specific interactions with host proteins and signaling cascades that distinguish these AOA from their close relatives from soil. Molecular signatures of Nitrosocosmicus ssp. were consistently identified on individuals in cross-sectional and longitudinal cohorts (n=47) with a higher prevalence in sebaceous areas. Co-occurrence network patterns with specific bacteria reinforce the observation that AOA of the genus Nitrosocosmicus form a stable component of the healthy skin and represent emerging commensals in the human microbiome.

show abstract

Section: Members Of the Genus Nitrosocosmicus Are Equipped For The Co...mentioning

confidence: 99%

Molecular Tracking and Cultivation Reveal Ammonia Oxidizing Archaea as Integral Members of the Human Skin Microbiome

Mahnert,

Dreer,

Perier

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The protonation of the anionic groups of these proteins with a decrease in the environmental pH leads to a decrease in their charge and an increase in their hydrophobicity, with subsequent embedding in the bilayer. These toxins and the chimeric polypeptide constructs based on them are capable of forming pores in artificial phospholipid membranes [ 209 , 210 , 211 , 212 , 213 ]. These pores can be seen on flat bilayers using atomic force microscopy [ 211 ].…”

Section: Branching Delivery Pathways For Endocytosed Drugsmentioning

confidence: 99%

Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells

Rosenkranz

Slastnikova

2023

Pharmaceutics

View full text Add to dashboard Cite

A large number of proteins are successfully used to treat various diseases. These include natural polypeptide hormones, their synthetic analogues, antibodies, antibody mimetics, enzymes, and other drugs based on them. Many of them are demanded in clinical settings and commercially successful, mainly for cancer treatment. The targets for most of the aforementioned drugs are located at the cell surface. Meanwhile, the vast majority of therapeutic targets, which are usually regulatory macromolecules, are located inside the cell. Traditional low molecular weight drugs freely penetrate all cells, causing side effects in non-target cells. In addition, it is often difficult to elaborate a small molecule that can specifically affect protein interactions. Modern technologies make it possible to obtain proteins capable of interacting with almost any target. However, proteins, like other macromolecules, cannot, as a rule, freely penetrate into the desired cellular compartment. Recent studies allow us to design multifunctional proteins that solve these problems. This review considers the scope of application of such artificial constructs for the targeted delivery of both protein-based and traditional low molecular weight drugs, the obstacles met on the way of their transport to the specified intracellular compartment of the target cells after their systemic bloodstream administration, and the means to overcome those difficulties.

show abstract

Protons at bio-interfaces

Bondar

Barboiu

2023

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Cryo-EM elucidates mechanism of action of bacterial pore-forming toxins

Cited by 6 publications

References 65 publications

Molecular Tracking and Cultivation Reveal Ammonia Oxidizing Archaea as Integral Members of the Human Skin Microbiome

Molecular Tracking and Cultivation Reveal Ammonia Oxidizing Archaea as Integral Members of the Human Skin Microbiome

Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells

Protons at bio-interfaces

Contact Info

Product

Resources

About