Cry1Ac Transgenic Sugarcane Does Not Affect the Diversity of Microbial Communities and Has No Significant Effect on Enzyme Activities in Rhizosphere Soil within One Crop Season

Zhou, Dinggang; Xu, Liping; Gao, Shaopei; Guo, Jinlong; Luo, Jun; You, Qi; Que, Youxiong

doi:10.3389/fpls.2016.00265

Cited by 21 publications

(12 citation statements)

References 73 publications

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“…No significant differences were observed in abundance of phyla between CC and TC-10 except for TC-15mix ( Figure 1 ). Our results were consistent with the previous publications, suggesting that monoculture of one transgenic cotton line may have no effect on fungal phylum composition in comparison with conventional cotton ( Griffiths et al, 2000 ; Koch et al, 2015 ; Zhou et al, 2016 ). However, a remarkable difference was observed between TC-15mix and groups of CC and TC-10 at phylum level.…”

Section: Discussionsupporting

confidence: 93%

Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons

Liu

Song

et al. 2016

Front. Plant Sci.

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Long-term growth of genetically modified plants (GMPs) has raised concerns regarding their ecological effects. Here, FLX-pyrosequencing of region I (18S) and region II (ITS1, 5.8S, and ITS2) rDNA was used to characterize fungal communities in soil samples after 10-year monoculture of one representative transgenic cotton line (TC-10) and 15-year plantation of various transgenic cotton cultivars (TC-15mix) over four seasons. Soil fungal communities in the rhizosphere of non-transgenic control (CC) were also compared. No notable differences were observed in soil fertility variables among CC, TC-10, and TC-15mix. Within seasons, the different estimations were statistically indistinguishable. There were 411 and 2 067 fungal operational taxonomic units in the two regions, respectively. More than 75% of fungal taxa were stable in both CC and TC except for individual taxa with significantly different abundance between TC and CC. Statistical analysis revealed no significant differences between CC and TC-10, while discrimination of separating TC-15mix from CC and TC-10 with 37.86% explained variance in PCoA and a significant difference of Shannon indexes between TC-10 and TC-15mix were observed in region II. As TC-15mix planted with a mixture of transgenic cottons (Zhongmian-29, 30, and 33B) for over 5 years, different genetic modifications may introduce variations in fungal diversity. Further clarification is necessary by detecting the fungal dynamic changes in sites planted in monoculture of various transgenic cottons. Overall, we conclude that monoculture of one representative transgenic cotton cultivar may have no effect on fungal diversity compared with conventional cotton. Furthermore, the choice of amplified region and methodology has potential to affect the outcome of the comparison between GM-crop and its parental line.

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Section: Discussionsupporting

confidence: 93%

Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons

Liu

Song

et al. 2016

Front. Plant Sci.

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“…Similarly, Bt cotton cultivation did not affect microbial communities in the rhizosphere soil [14] and the community characteristics of rhizosphere soil nematodes [13]. And Cry1Ac transgenic sugarcane would not change the diversity of microbial communities or modify enzyme activities in rhizosphere soil within one crop season [45]. Bt rice also had no persistent or adverse effect on the microbial community composition in its rhizosphere [12, 29].…”

Section: Discussionmentioning

confidence: 99%

High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils

Zhang

Shen

et al. 2019

PLoS ONE

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Potential environmental risks of genetically modified (GM) crops have raised concerns. To better understand the effect of transgenic rice on the bacterial community in paddy soil, a field experiment was carried out using pairs of rice varieties from two subspecies (indica and japonica) containing bar transgene with herbicide resistance and their parental conventional rice. The 16S rRNA gene of soil genomic DNA from different soil layers at the maturity stage was sequenced using high-throughput sequencing on the Illumina MiSeq platform to explore the microbial community diversity among different rice soils. There were no significant differences in diversity indices between transgenic japonica rice and its sister conventional rice (japonica pair) among different soil layers, but, significant differences was observed between transgenic indica rice and its conventional rice (indica pair) in the topsoil layer around concentrated rice roots according to the ace diversity index. Though the japonica rice soil and indica rice soil were shared several key genera, including Rivibacter, Anaeromyxobacter, Roseomonas, Geobacter, Thiobacillus, Clostridium, and Desulfobulbus, the primary bacterial genera in indica rice soil were different from those in japonica rice. Synechococcus and Dechloromonas were present in japonica rice samples, while Chloronema, Flexibacter, and Blastocatella were observed in indica rice soil. Moreover, the abundance of genera between GM and non-GM varieties in japonica rice was significantly different from indica rice, and several bacterial communities influenced these differences. Anaerovorax was more abundant in transgenic japonica rice soil than conventional rice soil, while it was deficient in transgenic indica rice soil compared to conventional rice soil, and opposite responses to Deferrisoma were in that of indica rice. Thus, we concluded that transgenic indica and japonica rice had different effects on soil bacteria compared with their corresponding sister conventional rice. However, these composition and abundance difference only occurred for a few genera but had no effect on the primary genera and soil characteristics were mainly contributed to these differences. Thus, differences in bacterial community structure can be ignored when evaluating the impacts of transgenic rice in the complex soil microenvironment.

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“…Fieldcultivated Bt transgenic cotton has also been found to have no signi cant effect on rhizosphere communities compared with WT cotton [74]. Similarly, Cry1Ac-sugarcane was found to have no impact on rhizosphere microbial diversity or enzyme activity compared with NT sugarcane within a single crop season [75]. In addition, no persistent or adverse effects on the rhizosphere bacterial community population were detected between WT and Bt-modi ed rice [76].…”

Section: Effects Of Cry1ah1 Expression On Native Rhizosphere Communitiesmentioning

confidence: 93%

Effects of Field-Grown Transgenic Cry1Ah1 Poplar on the Rhizosphere Microbiome

Wei

Movahedi

Pei-jun

et al. 2020

Preprint

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Background: Poplar (Populus) is a genus of globally important plantation trees used widely in industrial and agricultural production. However, poplar is easily damaged by Micromelalopha troglodyta and Hyphantria cunea, resulting in a decline in poplar quality. Due to their strong insect resistance, Bt toxin-encoded Cry genes have been widely adopted in poplar breeding; however, potential adverse effects of Cry1Ah1-modified poplars on the ecological environment have raised concerns. Results: In this study, we comprehensively analyzed the structural and functional composition of the rhizosphere microbiome in field-grown transgenic Bt poplar. Conclusions: Our analysis of soil chemistry patterns revealed that soil alkaline nitrogen, soil available phosphorus, and microbial biomass nitrogen and phosphorus levels were improved, whereas microbial biomass carbon declined in Cry1Ah1-modified poplar rhizosphere samples. We applied metagenomic sequencing of Non-Transgenic (NT) and Cry1Ah1-modified poplar rhizosphere samples collected from a natural field; the predominant taxa included Proteobacteria, Acidobacteria, and Actinobacteria. We also identified microbial functional traits involved in membrane transport, amino acid metabolism, carbohydrate metabolism, and replication and repair in NT and Cry1Ah1-modified poplars. Together, these results demonstrate that the NT and Cry1Ah1-modified poplar rhizosphere microbiomes had similar diversity and structure. These differences in relative abundance were observed in a few genera but did not affect the primary genera or soil.

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Cry1Ac Transgenic Sugarcane Does Not Affect the Diversity of Microbial Communities and Has No Significant Effect on Enzyme Activities in Rhizosphere Soil within One Crop Season

Cited by 21 publications

References 73 publications

Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons

Assessing Fungal Population in Soil Planted with Cry1Ac and CPTI Transgenic Cotton and Its Conventional Parental Line Using 18S and ITS rDNA Sequences over Four Seasons

High-throughput sequencing analysis of microbial community diversity in response to indica and japonica bar-transgenic rice paddy soils

Effects of Field-Grown Transgenic Cry1Ah1 Poplar on the Rhizosphere Microbiome

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