Cry10Aa Protein is Highly Toxic to &lt;i&gt;Anthonomus grandis&lt;/i&gt; Boheman (Coleoptera: Curculionidae), an Important Insect Pest in Brazilian Cotton Crop Fields

Souza, Raimundo Wagner de; Martins, E. S.; Ribeiro, Bergmann Morais; Gomes, Rose

doi:10.5376/bt.2012.03.0004

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…Craveiro et al (2010) reported the efficient biological control of sugarcane giant borer caused by the Lepidopteran larvae Telchin licus licus (Castniidae) by variants of Cry1Ia toxins. Other studies reported the high toxicity of B. thuringiensis Cry protein towards Anthonomus grandis Boheman (Coleoptera: Curculionidae) (de Souza Aguiar et al, 2012) and towards Cylas puncticollis and Cylas brunneus (Coleoptera: Brentidae) (Ekobu et al, 2010).…”

Section: Insecticidal Activity Of B Thuringiensis Derived Biopesticidesmentioning

confidence: 98%

Potential of bacterial derived biopesticides in pest management

2015

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Section: Insecticidal Activity Of B Thuringiensis Derived Biopesticidesmentioning

confidence: 98%

Potential of bacterial derived biopesticides in pest management

2015

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“…Different chemical insecticides have been used for its control such as Calcium arsenate, dichloro-diphenyl-trichloroethane (DDT) and organophosphates. This insect pest is susceptible to several Cry proteins such as Cry1Ia, Cry8Ka, Cry10Aa and Cry22Aa [41][42][43][44]. ABC transporters as well as APN and CAD have been identified as binding proteins form Cry8Ea in H. parallela and H. oblita [45,46].…”

Section: Plos Onementioning

confidence: 99%

Identification of Cry toxin receptor genes homologs in a de novo transcriptome of Premnotrypes vorax (Coleoptera: Curculionidae)

Velásquez C.,

Cantón,

Sanchez-Flores

et al. 2023

PLoS ONE

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The white potato worm Premnotrypes vorax (Hustache) (Coleoptera: Curculionidae) is one of the most destructive insect pests of potato crops in South America. Like many coleopteran insects, P. vorax shows low susceptibility to Cry insecticidal proteins produced by the bacterium Bacillus thuringiensis (Bt). However, the presence of Cry toxin receptors in the midgut of this this insect has never been studied. The main Cry-binding proteins described in other insect species are cadherin (CAD), aminopeptidase N (APN), alkaline phosphatase (ALP) and ATP-binding cassette (ABC) transporters. In this study, we analyzed and validated a de novo assembled transcriptome of Illumina sequencing data to identify and to characterize homologs of Cry toxin receptors. We identified the protein sequences in P. vorax that show high identity with their orthologous sequences of the Cry toxin binding proteins in other coleopteran larvae such as APN, ALP, CAD and ABC transporter. This study provides preliminary identification of putative receptor genes of Cry proteins that would be useful for future studies involving biocontrol of this important potato crop pest.

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“…The colonies of A. aegypti and C. quinquefasciatus were established from insects collected from the field in regions of transition between the urban and rural areas in the state of Tocantins, Brazil, (11°40′55.7″ latitude S, 49°04′3.9″ longitude W), where no insecticides have been used for the control of mosquitoes. The insects were maintained in the Entomology Laboratory of the Federal University of Tocantins, Gurupi Campus, according to the methodology described previously 44 . Adult mosquitoes were maintained on a 10% aqueous sucrose solution and the blood of live Wistar rats (Rattus norvegicus albinus).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

Alves

Melo

Oliveira

et al. 2020

Sci Rep

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Bacillus thuringiensis serovar israelensis (Bti) is used to control insect vectors of human and animal diseases. in the present study, the toxicity of four strains of Bti, named T0124, T0131, T0137, and T0139, toward Aedes aegypti and Culex quinquefasciatus larvae was analyzed. The T0131 strain showed the highest larvicidal activity against A. aegypti (Lc 50 = 0.015 µg/ml) and C. quinquefasciatus larvae (Lc 50 = 0.035 µg/ml) when compared to the other strains. Furthermore, the genomic sequences of the four strains were obtained and compared. These Bti strains had chromosomes sizes of approximately 5.4 Mb with GC contents of ~35% and 5472-5477 putative coding regions. Three small plasmids (5.4, 6.8, and 7.6 kb) and three large plasmids (127, 235, and 359 kb) were found in the extrachromosomal content of all four strains. the Snp-based phylogeny revealed close relationship among isolates from this study and other Bti isolates, and SNPs analysis of the plasmids 127 kb did not reveal any mutations in δ-endotoxins genes. This newly acquired sequence data for these Bti strains may be useful in the search for novel insecticidal toxins to improve existing ones or develop new strategies for the biological control of important insect vectors of human and animal diseases.

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Cry10Aa Protein is Highly Toxic to <i>Anthonomus grandis</i> Boheman (Coleoptera: Curculionidae), an Important Insect Pest in Brazilian Cotton Crop Fields

Cited by 8 publications

References 38 publications

Potential of bacterial derived biopesticides in pest management

Potential of bacterial derived biopesticides in pest management

Identification of Cry toxin receptor genes homologs in a de novo transcriptome of Premnotrypes vorax (Coleoptera: Curculionidae)

Comparative genomic analysis and mosquito larvicidal activity of four Bacillus thuringiensis serovar israelensis strains

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