Crustacean hemolymph microbiota: Endemic, tightly controlled, and utilization expectable

Wang, Xianwei; Wang, Jinxing

doi:10.1016/j.molimm.2015.06.018

Cited by 60 publications

(48 citation statements)

References 80 publications

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“…Our demonstration that the Biomphalaria hemolymph contains a rich microbiome is consistent with published reports detailing the microbiome from other mollusks, crustaceans [19, 20, 31, 32] and also from insects [33]. The intimate relationship between these organisms and their water environment and their semi-open vascular system may prevent them from having a sterile hemolymph.…”

Section: Discussionsupporting

confidence: 91%

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

Chevalier

Diaz

McDew-White

et al. 2020

Preprint

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The microbiome – the microorganism community that is found on or within an organism’s body – is increasingly recognized to shape many aspects of its host biology and is a key determinant of health and disease. Microbiomes modulate the capacity of insect disease vectors (mosquitos, tsetse flies, sandflies) to transmit parasites and disease. We investigate the diversity and abundance of microorganisms within the hemolymph (i.e. blood) of Biomphalaria snails, the intermediate host for Schistosoma mansoni, using Illumina MiSeq sequencing of the bacterial 16S V4 rDNA. We sampled hemolymph from 5 snails from 6 different laboratory populations of B. glabrata and one population of B. alexandrina. We observed 281.34 ± 0.81 amplicon sequence variants (ASVs) per snail. There were significant differences in microbiome composition at the level of individual snails, snail populations and species. Snail microbiomes were dominated by Proteobacteria and Bacteroidetes while water microbiomes from snail tank were dominated by Actinobacteria. We investigated the absolute bacterial load using qPCR: hemolymph samples contained 2,620 ± 321 bacteria per μL. We speculate that the microbiome may represent a critical, but unexplored intermediary in the snail-schistosome interaction as hemolymph is in very close contact to the parasite at each step of its development.

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Section: Discussionsupporting

confidence: 91%

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

Chevalier

Diaz

McDew-White

et al. 2020

Preprint

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“…The external factor, A. bombi infection, can influence microbial composition and was thereby statistically analysed on all 57 characterized bacterial phylotypes residing in the fat body using the mvabund (v.3.11.5) package for R (Wang et al, ; Wang and Wang, ). Also the factor location and its interaction with A. bombi can influence microbial composition and were as such integrated in the generalized linear model (GLM).…”

Section: Resultsmentioning

confidence: 99%

“…Supporting Information), counting 12 out of 17 bumblebees in the first location, called Sterre, and 9 out of 18 bumblebees in the second location, called Bramier, which revealed no significant prevalence differences (χ 2 = 0.80542, df = 1, p = 0.3695). The external factor, A. bombi infection, can influence microbial composition and was thereby statistically analysed on all 57 characterized bacterial phylotypes residing in the fat body using the mvabund (v.3.11.5) package for R (Wang et al, 2012;Wang and Wang, 2015). Also the factor location and its interaction with A. bombi can influence microbial composition and were as such integrated in the generalized linear model (GLM).…”

Section: Variable Bacterial Community In the Fat Body Of Bumblebeesmentioning

confidence: 99%

A prokaryotic–eukaryotic relation in the fat body of Bombus terrestris

Parmentier

Billiet

Smagghe

et al. 2018

Environ Microbiol Rep

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The interaction between the insect host and its microbiota plays a central role in insect health and is mostly studied in relation to the digestive system. Nonetheless, there are numerous microorganisms occupying multiple habitats in and on insects. We studied microbial communities in the gut and fat body of bumblebees (Bombus terrestris) using the V4 region of the 16S rRNA gene on the Illumina MiSeq platform. In one of the two study locations, the fat body microbial composition was marked by the dominant presence of Arsenophonus sp. and Phyllobacterium sp. Bumblebees infected with Apicystis bombi, a eukaryotic parasite multiplying in the fat body, had a significant higher relative abundance of Arsenophonus sp. compared with the non-infected individuals. In general, the infection of A. bombi correlated with a more interlinked microbial association network, as we observed an increase of significant associations between the relative abundance of bacteria present in the gut and fat body of infected bumblebees. The causality within this potential prokaryotic-eukaryotic relation is important when assessing the health impact on bees.

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“…The high abundance of Vibrio in particular is consistent with microbiomes of other marine invertebrates such as copepods [ 35 ] or sea urchins [ 73 ]. Vibrio is a very common endo- and epibiont in marine crustaceans and often produces chitinolytic enzymes that allow for exploiting chitinous exoskeleton as a niche for attachment and proliferation [ 71 , 74 , 75 ]. Some Vibrio species are pathogens and others have been implicated in biogeochemical processes, but the specific metabolic relationships between crustaceans and Vibrio are cryptic [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiome composition within a sympatric species complex of intertidal isopods (Jaera albifrons)

2018

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The increasingly recognised effects of microbiomes on the eco-evolutionary dynamics of their hosts are promoting a view of the “hologenome” as an integral host-symbiont evolutionary entity. For example, sex-ratio distorting reproductive parasites such as Wolbachia are well-studied pivotal drivers of invertebrate reproductive processes, and more recent work is highlighting novel effects of microbiome assemblages on host mating behaviour and developmental incompatibilities that underpin or reinforce reproductive isolation processes. However, examining the hologenome and its eco-evolutionary effects in natural populations is challenging because microbiome composition is considerably influenced by environmental factors. Here we illustrate these challenges in a sympatric species complex of intertidal isopods (Jaera albifrons spp.) with pervasive sex-ratio distortion and ecological and behavioural reproductive isolation mechanisms. We deep-sequence the bacterial 16S rRNA gene among males and females collected in spring and summer from two coasts in north-east Scotland, and examine microbiome composition with a particular focus on reproductive parasites. Microbiomes of all species were diverse (overall 3,317 unique sequences among 3.8 million reads) and comprised mainly Proteobacteria and Bacteroidetes taxa typical of the marine intertidal zone, in particular Vibrio spp. However, we found little evidence of the reproductive parasites Wolbachia, Rickettsia, Spiroplasma and Cardinium, suggesting alternative causes of sex-ratio distortion. Notwithstanding, a significant proportion of the variance in microbiome composition among samples was explained by sex (14.1 %), nested within geographic (26.9 %) and seasonal (39.6 %) variance components. The functional relevance of this sex signal was difficult to ascertain given the absence of reproductive parasites, the ephemeral nature of the species assemblages and substantial environmental variability. These results establish the Jaera albifrons species complex as an intriguing system for examining the effects of microbiomes on reproductive processes and speciation, and highlight the difficulties associated with snapshot assays of microbiome composition in dynamic and complex environments.

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Crustacean hemolymph microbiota: Endemic, tightly controlled, and utilization expectable

Cited by 60 publications

References 80 publications

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

The hemolymph ofBiomphalariasnail vectors of schistosomiasis supports a diverse microbiome

A prokaryotic–eukaryotic relation in the fat body of Bombus terrestris

Microbiome composition within a sympatric species complex of intertidal isopods (Jaera albifrons)

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