“Crustacea”: Comparative Aspects of Early Development

Wolff, Carsten; Gerberding, Matthias

doi:10.1007/978-3-7091-1853-5_2

Cited by 22 publications

(16 citation statements)

References 76 publications

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“…During germband formation, the ectoderm contributing to the posterior head and the trunk became organized in a stereotyped grid-like pattern with ordered AP rows and DV columns of cells ( Figure 2A–A’’ ) ( Browne et al, 2005 ; Dohle et al, 2004 ; Dohle and Scholtz, 1988 ; Gerberding et al, 2002 ; Scholtz, 1990 ; Wolff and Gerberding, 2015 ). Each row of cells corresponded to one parasegment, which is the unit of early metameric organization in Parhyale embryos, like in Drosophila and other arthropods ( Hejnol and Scholtz, 2004 ; Scholtz et al, 1994 ).…”

Section: Resultsmentioning

confidence: 99%

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Wolff

Tinévez

Pietzsch

et al. 2018

eLife

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143

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During development, coordinated cell behaviors orchestrate tissue and organ morphogenesis. Detailed descriptions of cell lineages and behaviors provide a powerful framework to elucidate the mechanisms of morphogenesis. To study the cellular basis of limb development, we imaged transgenic fluorescently-labeled embryos from the crustacean Parhyale hawaiensis with multi-view light-sheet microscopy at high spatiotemporal resolution over several days of embryogenesis. The cell lineage of outgrowing thoracic limbs was reconstructed at single-cell resolution with new software called Massive Multi-view Tracker (MaMuT). In silico clonal analyses suggested that the early limb primordium becomes subdivided into anterior-posterior and dorsal-ventral compartments whose boundaries intersect at the distal tip of the growing limb. Limb-bud formation is associated with spatial modulation of cell proliferation, while limb elongation is also driven by preferential orientation of cell divisions along the proximal-distal growth axis. Cellular reconstructions were predictive of the expression patterns of limb development genes including the BMP morphogen Decapentaplegic.

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Section: Resultsmentioning

confidence: 99%

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Wolff

Tinévez

Pietzsch

et al. 2018

eLife

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143

137

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“…We next asked whether Toll genes have a conserved function in germband extension outside of the insects, something that seems unlikely given the considerable diversity in modes of embryogenesis [23][24][25] and in Toll family genes [26] between non-insect arthropods. To answer this question, we identified and analyzed the relevant Toll genes in representatives of each of the three major arthropod lineages: crustaceans, myriapods, and chelicerates, each of which diverged approximately 525, 550, and 560 Ma, respectively [19].…”

Section: Toll Genes Exhibit Striped Expression In a Diverse Range Of mentioning

confidence: 99%

Toll Genes Have an Ancestral Role in Axis Elongation

et al. 2016

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One of the key morphogenetic processes used during development is the controlled intercalation of cells between their neighbors. This process has been co-opted into a range of developmental events, and it also underlies an event that occurs in each major group of bilaterians: elongation of the embryo along the anterior-posterior axis [1]. In Drosophila, a novel component of this process was recently discovered by Paré et al., who showed that three Toll genes function together to drive cell intercalation during germband extension [2]. This finding raises the question of whether this role of Toll genes is an evolutionary novelty of flies or a general mechanism of embryonic morphogenesis. Here we show that the Toll gene function in axis elongation is, in fact, widely conserved among arthropods. First, we functionally demonstrate that two Toll genes are required for cell intercalation in the beetle Tribolium castaneum. We then show that these genes belong to a previously undescribed Toll subfamily and that members of this subfamily exhibit striped expression (as seen in Tribolium and previously reported in Drosophila [3-5]) in embryos of six other arthropod species spanning the entire phylum. Last, we show that two of these Toll genes are required for normal morphogenesis during anterior-posterior embryo elongation in the spider Parasteatoda tepidariorum, a member of the most basally branching arthropod lineage. From our findings, we hypothesize that Toll genes had a morphogenetic function in embryo elongation in the last common ancestor of all arthropods, which existed over 550 million years ago.

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“…The life history of Parhyale makes it a versatile model organism amenable to experimental manipulations (Figure 1C) (Wolff and Gerberding, 2015). Gravid females lay eggs every 2 weeks upon reaching sexual maturity and hundreds of eggs can be easily collected at all stages of embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

The genome of the crustacean Parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion

Kao

Lai

Stamataki

et al. 2016

eLife

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The amphipod crustacean Parhyale hawaiensis is a blossoming model system for studies of developmental mechanisms and more recently regeneration. We have sequenced the genome allowing annotation of all key signaling pathways, transcription factors, and non-coding RNAs that will enhance ongoing functional studies. Parhyale is a member of the Malacostraca clade, which includes crustacean food crop species. We analysed the immunity related genes of Parhyale as an important comparative system for these species, where immunity related aquaculture problems have increased as farming has intensified. We also find that Parhyale and other species within Multicrustacea contain the enzyme sets necessary to perform lignocellulose digestion ('wood eating'), suggesting this ability may predate the diversification of this lineage. Our data provide an essential resource for further development of Parhyale as an experimental model. The first malacostracan genome will underpin ongoing comparative work in food crop species and research investigating lignocellulose as an energy source.DOI: http://dx.doi.org/10.7554/eLife.20062.001

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“Crustacea”: Comparative Aspects of Early Development

Cited by 22 publications

References 76 publications

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Multi-view light-sheet imaging and tracking with the MaMuT software reveals the cell lineage of a direct developing arthropod limb

Toll Genes Have an Ancestral Role in Axis Elongation

The genome of the crustacean Parhyale hawaiensis, a model for animal development, regeneration, immunity and lignocellulose digestion

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