Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India

Das, Kishore; Mukherjee, Ashis K.

doi:10.1016/j.biortech.2006.05.032

Cited by 564 publications

(269 citation statements)

References 33 publications

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“…This has led to increased toxicity and bioaccumulation of Petroleum hydrocarbon in the seafoods from the mangroves (Oudot et al, 1981;Vandermeulen and Gilfillan, 1985;Odokuma and Dickson, 2003). Among the various strategies adopted to clean petroleum oil-contaminated soil, bioremediation is recognized as one of the effective and inexpensive technologies (Das and Mukherjee, 2007). This approach involves supplying hydrocarbon-degrading efficient microorganisms to degrade target compounds, along with appropriate nutrients, to the subsurface (petroleum oil-contaminated sites).…”

Section: Ajesmentioning

confidence: 99%

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BIOREMEDIATION OF PETROLEUM HYDROCARBON-POLLUTED MANGROVE SWAMPS USING NUTRIENT FORMULA PRODUCED FROM WATER HYACINT (<I>EICCHORNIA CRASSIPES</I>)

Orji¹,

Ibiene

Okerentugba

2013

American Journal of Environmental Sciences

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Laboratory-scale studies were carried out using a nutrient formula produced from Eicchornia crassipes plant to achieve bioremediation of crude oil impacted mangrove soil. In a 70 day study, the culturable heterotrophic bacterial population of the Eichhornia crassipes recipe increased from 6.26×105 Cfu/g to 2.69×10 7 Cfu/g. The control set-up had its total culturable bacterial count increased from 5.76×10 5 Cfu/g to 1.24×10 6 Cfu/g. Statistical analyses showed significant difference for the two conditions (p ≥ 0.05). The total culturable heterotrophic fungal count in the Eichhornia crassipes recipe treatment increased from 5.36×105 Cfu/g to 2.50×10 7 Cfu/g respectively. The total culturable hydrocarbon utilising bacteria in Eichhornia crassipes treated polluted mangrove soil increased from 2.52×104 Cfu/g to 3.81×10 7 Cfu/g. Statistical analyses showed significant difference at p ≥ 0.05 level for the two conditions (Eicchornia crassipes nutrient treated soil and control). The total culturable hydrocarbon utilising fungal counts increased significantly for both the control and Eichhornia crassipes treatment. There was no regular trend in pH changes in all the conditions. The conductivity value of the Eichhornia crassipes recipe treated soil decreased progressively. Phosphate, nitrate, %total organic carbon, Total Hydrocarbon Content (THC). Studies using Gas chromatographic analyses showed that in the Eichhornia crassipes recipe treated polluted mangrove soil, 0, 58.92 and 75.36% were lost at zero hour, 28th day and 70th day respectively. In addition, in the control experimental set-up, 0, 7.14 and 13.42% of TPH were lost at zero hour, 28th day and 70th day respectively. There was no significant difference between the control experiment and Eichhornia crassipes (p = 0.054). The use of organic nutrient sources such Eichhornia crassipes recipe/nutrient powder is of good use as source of limiting nutrient needed for bioremediation of crude oil impacted medium.

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Section: Ajesmentioning

confidence: 99%

“…Crude petroleum oil and its derivatives released in the environment either accidentally or deliberately pose problems of increasing magnitude throughout the world (Okoh and Trejo-Hernandez, 2006). Furthermore, these problems are more aggravated because of the expensive disposal methods (Rahman et al, 2003;Das and Mukherjee, 2007). The…”

Section: Introductionmentioning

confidence: 99%

BIOREMEDIATION OF PETROLEUM HYDROCARBON-POLLUTED MANGROVE SWAMPS USING NUTRIENT FORMULA PRODUCED FROM WATER HYACINT (<I>EICCHORNIA CRASSIPES</I>)

Orji¹,

Ibiene

Okerentugba

2013

American Journal of Environmental Sciences

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“…A study has agreed with this present observation that it has no significant difference in the yield of biosurfactant was noticed between 48 h and 96 h of bacterial stationery growth phase. It was suggesting that the produced lipopeptide biosurfactant by B. subtilis DM-03 and DM-04 strains are growth dependent production (Das and Mukherjee, 2007).…”

Section: Discussionmentioning

confidence: 99%

A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

2016

Int. J. Adv. Res. Biol. Sci.

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A potential biosurfactant producing halophilic bacterium was isolated from the saltpan environment of Porto Novo, Tamilnadu, India. The potential bacterium showed 69.9 % emulsification activity, 56.7 units/ml lipase activity and 22 mm oil displacement in the biosurfactant multiple screening methods. This halophilic bacterium was identified as Bacillus species using 16S rRNA molecular sequencing method. This strain showed growth dependent biosurfactant production, which was partially purified and weighed at 0.945 ±0.021 g/L on dry weight basis. The isolated biosurfactant was biochemically identified as glycolipid using biochemical methods and FT-IR functional group analysis. Further, the glycolipid surfactant showed consistent oil recovery efficiency than the tested synthetic surfactant at the temperature up to 50°C and pH ranged from 5 to 8. Based on these observations, the isolated biosurfactant using this halophilic bacterium revealed promising microbially enhanced oil recovery and bioremediation purposes at a wide range of environmental conditions.

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“…Based on their ability to produce surfactant and break down hydrocarbons (Chayabutra and Yu, 2001;Gudina et al, 2013), P. aeruginosa and B. subtilis are often utilized for bioremediation purposes, but their activities are enhanced when they have been previously exposed to the pollutant (Das and Mukherjee, 2007;Kaczorek et al, 2010;Chandankere et al, 2013). The exposure of these species to 1% and 70% (v/v) concentrations of diesel mixture was meant to induce biotransformation that will improve their resistance to diesel as well as trigger the production of surfactants necessary for biodegradation (Tang et al, 2007).…”

Section: Comparative Adaptive Capabilities Of P Aeruginosa and B Sumentioning

confidence: 99%

Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

Fosso‐Kankeu

Marx

Brink

2017

Braz. J. Chem. Eng.

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-Two bacteria, namely Bacillus subtilis and Pseudomonas aeruginosa were exposed to different concentrations of diesel to increase their adaptation to the fossil fuel, and were used for the degradation of diesel-biodiesel blends. The biodegradation rate was evaluated using the redox indicator 2,6-dichlorophenol indophenol (DCPIP) test and gravimetric analysis. The preliminary exposure of cells to diesel proved to improve their biodegradation capacities, and exposure to a higher concentration (70%) of diesel resulted in maximum biodegradation of up to 58.38 g and 1.78 g of degraded oil per CFU/mL (10 10 ) for P. aeruginosa and B. subtilis respectively. It was found that the adapted cells preferably used diesel in the blend. P. aeruginosa and B. subtilis exhibited different adaptation capabilities and biodegradation behaviour. Biodiesel stimulated the biodegradation of the diesel-biodiesel blends by non-adapted cells only; the adapted cells exhibited a different behaviour.

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Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India

Cited by 564 publications

References 33 publications

BIOREMEDIATION OF PETROLEUM HYDROCARBON-POLLUTED MANGROVE SWAMPS USING NUTRIENT FORMULA PRODUCED FROM WATER HYACINT (<I>EICCHORNIA CRASSIPES</I>)

BIOREMEDIATION OF PETROLEUM HYDROCARBON-POLLUTED MANGROVE SWAMPS USING NUTRIENT FORMULA PRODUCED FROM WATER HYACINT (<I>EICCHORNIA CRASSIPES</I>)

A glycolipid biosurfactant from a native halophilic Bacillus species of Porto Novo, India and its potential microbially enhanced oil recovery properties

Adaptation behaviour of bacterial species and impact on the biodegradation of biodiesel-diesel

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