Crucial roles of reactive chemical species in modification of respiratory syncytial virus by nitrogen gas plasma

Sakudo, Akikazu; Toyokawa, Yoichi; Imanishi, Yuichiro; Murakami, Tomoyuki

doi:10.1016/j.msec.2017.02.007

Cited by 25 publications

(21 citation statements)

References 23 publications

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“…Its efficacy has been successfully demonstrated against viruses that cause foodborne illness such as norovirus as well as viruses that cause respiratory illness, including influenza, adenovirus, and respiratory syncytial virus, where NTP prevented viral replication and transmission. [ 78–85 ]…”

Section: Nonthermal Plasma (Ntp) As An Immunogenic Cell Death Inducermentioning

confidence: 99%

Nonthermal plasma as part of a novel strategy for vaccination

Mohamed

Esposito

Kutzler

et al. 2020

Plasma Processes & Polymers

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Vaccination has been one of the most effective health intervention mechanisms to reduce morbidity and mortality associated with infectious diseases. Vaccines stimulate the body's protective immune responses through controlled exposure to modified versions of pathogens that establish immunological memory. However, only a few diseases have effective vaccines. The biological effects of nonthermal plasma on cells suggest that plasma could play an important role in improving efficacy of existing vaccines and overcoming some of the limitations and challenges with current vaccination strategies. This review summarizes the opportunities for nonthermal plasma for immunization and therapeutic purposes.

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Section: Nonthermal Plasma (Ntp) As An Immunogenic Cell Death Inducermentioning

confidence: 99%

Nonthermal plasma as part of a novel strategy for vaccination

Mohamed

Esposito

Kutzler

et al. 2020

Plasma Processes & Polymers

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“…The Western blotting data therefore suggest that the nitrogen gas plasma degrades the A subunit of Stx, which is known to elicit its toxic activity. Therefore, some factors generated during operation of the BLP-TES device such as the production of reactive chemical species, which have previously been shown to be the principal contributing reason for the inactivation of bacteria and viruses (Maeda et al 2015; Sakudo et al 2017), may be responsible for degrading Stx and their A subunits. Additional clarification of the mechanisms by which nitrogen gas plasma degrades Stx are required.…”

Section: Discussionmentioning

confidence: 99%

“…Several factors contribute to the thermal and non-thermal mechanism of inactivation, such as heat, ultraviolet (UV) radiation, and reactive chemical species generated during operation of the nitrogen gas plasma device. Among them, reactive chemical species may be the most important factor for inactivation of bacteria and viruses (Maeda et al 2015; Sakudo et al 2017). …”

Section: Introductionmentioning

confidence: 99%

Degradation and inactivation of Shiga toxins by nitrogen gas plasma

Sakudo

Imanishi

2017

AMB Expr

Self Cite

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Shiga toxin (Stx)-producing Escherichia coli (STEC) leads to food poisoning by causing hemorrhagic colitis and hemolytic uremic syndrome. Some STEC produce Shiga toxin 1 (Stx1) and/or Shiga toxin 2 (Stx2), a relatively stable protein toxin, necessitating the development of an efficient inactivation method. Here we applied a nitrogen gas plasma apparatus to the inactivation of Stx. Samples of Stx1 and Stx2 were treated with a nitrogen gas plasma generated by a plasma device using a short high-voltage pulse applied by a static induction thyristor power supply at 1.5 kpps (kilo pulse per second). The recovered Stx samples were then analyzed for immunological and biological activities. Immunochromatography demonstrated that Stx1 and Stx2 were degraded by the gas plasma. Quantification by enzyme-linked immunosorbent assay (ELISA) showed that both toxins were efficiently degraded to less than 1/10th of their original concentration within 5 min of treatment. Western blotting further showed the gas plasma treatment degraded the A subunit, which mediates the toxicity of Stx. Moreover, an assay using HEp-2 cells as an index of cytotoxicity showed that gas plasma treatment reduced the toxic activity of Stx. Therefore, nitrogen gas plasma might be an efficient method for the inactivation of Stx.

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“…Researchers also use CAPs successfully inactivate various virus and mechanism has been investigated as well [49][50][51].…”

Section: Plasma Interaction With Microorganismsmentioning

confidence: 99%

Cold Atmospheric Pressure Plasmas (CAPs) for Skin Wound Healing

Xiong¹

2018

Plasma Medicine - Concepts and Clinical Applications

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In the past 20 years, cold atmospheric pressure plasmas (CAPs) have become a new promising way for many biomedical applications, such as disinfection, cancer treatment, root canal treatment, wound healing, and other medical applications. Among these applications, investigations of plasma for skin wound healing has gained huge success both in vitro and in vivo experiments, and also the mechanism behind it has been studied by many groups. In this chapter, we summarize the state-of-the-art progress in wound healing by CAPs. The plasma devices developed for wound healing, the interactions between plasmas and microorganisms/cells/tissues, the in vitro and in vivo treatments, the clinical trials, and biosafety issues are all included.

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Crucial roles of reactive chemical species in modification of respiratory syncytial virus by nitrogen gas plasma

Cited by 25 publications

References 23 publications

Nonthermal plasma as part of a novel strategy for vaccination

Nonthermal plasma as part of a novel strategy for vaccination

Degradation and inactivation of Shiga toxins by nitrogen gas plasma

Cold Atmospheric Pressure Plasmas (CAPs) for Skin Wound Healing

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