Crucial role of<i>Salmonella</i>genomic island 1 master activator in the parasitism of IncC plasmids

Durand, Romain; Huguet, Kévin; Rivard, Nicolas; Carraro, Nicolas; Rodrigue, Sébastien; Burrus, Vincent

doi:10.1093/nar/gkab204

Cited by 10 publications

(45 citation statements)

References 81 publications

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“…Retention of sgaC in the GIs could result from its essential role as the elicitor of excision and replication reported for SGI1. Indeed, although AcaCD binds to the promoters P xis and P rep of SGI1, it fails to initiate transcription at these two promoters, unlike SgaCD [28]. [26].…”

Section: Discussionmentioning

confidence: 99%

“…6A, 6B and 6C). We recently showed that AcaD most likely stabilizes the binding of AcaC to the DNA [28]. Therefore, AcaD and SgaC from MGIVchUSA3 likely interact to form a chimerical activator complex.…”

Section: Discussionmentioning

confidence: 99%

“…[26]. Multiple GIs also carry putative replicons based on repA and trfA (Fig 1), suggesting that independent replication is crucial in the lifecycle of these GIs, perhaps to improve their stability in the presence of their helper plasmid [26][27][28][29][30].…”

Section: Discussionmentioning

confidence: 99%

“…All final constructs were verified by PCR and DNA sequencing by the Plateforme de Séquençage et de Génotypage du Centre de Recherche du CHUL (Québec, QC, Canada). qPCR assays qPCR assays for quantification of excision and copy number of MGIVchUSA3 Kn were carried out as described previously [28] with the following modification.…”

Section: Plasmids and Strains Constructionsmentioning

confidence: 99%

“…The excision rate and copy number of MGIVchUSA3 Kn were calculated as the ratio of free attB dusA site per chromosome and as the ratio of higA per chromosome, respectively. The data were analyzed and normalized using all three chromosomal genes dnaB, hicB and trmE as references and the qBase framework as described previously [28,55].…”

Section: Plasmids and Strains Constructionsmentioning

confidence: 99%

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Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids

Durand

Deschênes

Burrus

2021

Preprint

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Salmonella Genomic Island 1 (SGI1) and its variants are significant contributors to the spread of antibiotic resistance among Gammaproteobacteria . All known SGI1 variants integrate at the 3’ end of trmE , a gene coding for a tRNA modification enzyme. SGI1 variants are mobilized specifically by conjugative plasmids of the incompatibility groups A and C (IncA and IncC). Using a comparative genomics approach based on genes conserved among members of the SGI1 group, we identified diverse genomic islands (GIs) distantly related to SGI1 in several species of Vibrio , Aeromonas , Salmonella , Pokkaliibacter , and Escherichia . Unlike SGI1, these GIs target two alternative chromosomal loci, the 5’ end of dusA and the 3’ end of yicC . Although these elements share many features with SGI1, they lack antibiotic resistance genes and carry alternative integration/excision modules. Functional characterization of MGI Vch USA3, a dusA -specific GI, revealed promoters that respond to AcaCD, the master activator of IncC plasmid transfer genes. Quantitative PCR and mating assays confirmed that MGI Vch USA3 excises from the chromosome and is mobilized by an IncC helper plasmid from Vibrio cholerae to Escherichia coli . MGI Vch USA3 encodes the AcaC homolog SgaC that associates with AcaD to form a hybrid activator complex AcaD/SgaC essential for its excision and mobilization. We identified the dusA -specific recombination directionality factor RdfN required for the integrase-mediated excision of dusA -specific GIs from the chromosome. Like xis in SGI1, rdfN is under the control of an AcaCD-responsive promoter. Although the integration of MGI Vch USA3 disrupts dusA , the GI provides a new promoter sequence and restores the reading frame of dusA for proper expression of the tRNA-dihydrouridine synthase A. Phylogenetic analysis of the conserved proteins encoded by SGI1-like elements targeting dusA , yicC , and trmE gives a fresh perspective on the possible origin of SGI1 and its variants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plasmids and Strains Constructionsmentioning

confidence: 99%

Section: Plasmids and Strains Constructionsmentioning

confidence: 99%

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Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids

Durand

Deschênes

Burrus

2021

Preprint

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Effect of the S008-sgaCD operon on IncC plasmid stability in the presence of SGI1-K or absence of an SGI1 variant

Ambrose

Hall

2023

Plasmid

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Origins of transfer establish networks of functional dependencies for plasmid transfer by conjugation

Ares-Arroyo

Coluzzi

Rocha

2022

Nucleic Acids Research

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Plasmids can be transferred between cells by conjugation, thereby driving bacterial evolution by horizontal gene transfer. Yet, we ignore the molecular mechanisms of transfer for many plasmids because they lack all protein-coding genes required for conjugation. We solved this conundrum by identifying hundreds of plasmids and chromosomes with conjugative origins of transfer in Escherichia coli and Staphylococcus aureus. These plasmids (pOriT) hijack the relaxases of conjugative or mobilizable elements, but not both. The functional dependencies between pOriT and other plasmids explain their co-occurrence: pOriT are abundant in cells with many plasmids, whereas conjugative plasmids are the most common in the others. We systematically characterized plasmid mobility in relation to conjugation and alternative mechanisms of transfer and can now propose a putative mechanism of transfer for ∼90% of them. In most cases, plasmid mobility seems to involve conjugation. Interestingly, the mechanisms of mobility are important determinants of plasmid-encoded accessory traits, since pOriTs have the highest densities of antimicrobial resistance genes, whereas plasmids lacking putative mechanisms of transfer have the lowest. We illuminate the evolutionary relationships between plasmids and suggest that many pOriT may have arisen by gene deletions in other types of plasmids. These results suggest that most plasmids can be transferred by conjugation.

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Crucial role ofSalmonellagenomic island 1 master activator in the parasitism of IncC plasmids

Cited by 10 publications

References 81 publications

Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids

Genomic islands targeting dusA in Vibrio species are distantly related to Salmonella Genomic Island 1 and mobilizable by IncC conjugative plasmids

Effect of the S008-sgaCD operon on IncC plasmid stability in the presence of SGI1-K or absence of an SGI1 variant

Origins of transfer establish networks of functional dependencies for plasmid transfer by conjugation

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