Crucial factors in biosynthesis of fluorescent CdSe quantum dots in Saccharomyces cerevisiae

Abstract

Simple, controllable and repeatable procedures to biosynthesize CdSe in Saccharomyces cerevisiae are systematically demonstrated.

Biosynthesis of cadmium selenide quantum dots by Providencia vermicola

“…It is worth mentioning that, the reaction of P. vermicola BGRW culture with cadmium chloride (Tube 2) at ( Figure 13B) did not emit any fluorescence light under UV light which shows the quantum dots did not produce, unless the existence of cadmium and selenium ions together and the type of QDs were CdSe, not CdS. In addition, it is worth mentioning that the color of fluorescent lighting is different by various ratios, which showed that the concentration of materials effect on the size of the quantum as known, the fluorescent color differs depending on the QDs size similar to other results (Wu et al, 2015).…”

“…The flasks were incubated on a shaker at 110 rpm and 37°C for 24 h according to Suresh et al (2014) with slight modification. The synthesis of QDs was followed by visually observation of gradation color from red to orange-yellow formed in the culture, UV-Vis and fluorescence spectrums were also obtained as previously described (Wu et al, 2015).…”

“…Similar to spectra of CdSe, QDs showed the presence of absorbance band centered at 350 nm in Gupta and Ramrakhiani (2009) and Suresh (2014) studies. When the biological method of CdSe QDs production was used, an absorption band may be centered between 270 and 280 nm, which is visible due to protein or peptide, associated with the quantum dots; indicating a possible protein or a peptide core-shell as a capping molecule (Wu et al, 2015). The broad peak of QDs may be due to the variation in the size of the nanoparticles (Malarkodi et al, 2014).…”

“…strain RB were 10-20 nm. Recently, 15 to 20 nm CdSe QDs were biosynthesized by yeast (Wu et al, 2015).…”

In Vivo Biosynthesis of Inorganic Nanomaterials Using Eukaryotes—A Review

“…In addition to corroborating the findings of Cui et al regarding the use of yeast cells at their stationary phase of growth [ 65 ], Wu et al investigated the optimization of CdSe QD synthesis using live cells of S. cerevisiae by screening the impact of the time of addition, the amounts and incubation time of Na 2 SeO 3 and CdCl 2 with Na 2 SeO 3 [ 124 ]. In another study, Bao et al used the same species of yeast to carry out the fabrication of well-dispersed CdTe QDs of ~3.6 nm in diameter [ 8 ].…”