CRSBP‐1/LYVE‐l‐null mice exhibit identifiable morphological and functional alterations of lymphatic capillary vessels

Huang, Shuan Shian; Liu, I-Hua; Smith, Tracy J.; Shah, Maulik; Johnson, Frank E.; Huang, Jung San

doi:10.1016/j.febslet.2006.10.028

Cited by 35 publications

(70 citation statements)

References 31 publications

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“…Their technique for discrimination of lymphatic vessels is highly valuable. Recently, more lymphatic endothelial markers for specifying the presence of lymphatic vessels have become available [8][9][10][11][12][13][14][15] . Podoplanin, whose expression was examined in the present study, is one of the proteins most highly expressed in the lymphatic system, although its expression was first defined in kidney glomerular epithelial cells 14,15) .…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of the Lymphatic Vessels in Major Salivary Glands of the Rat

Aiyama

Kikuchi

Takada

et al. 2011

Okajimas Folia Anat. Jpn.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical Study of the Lymphatic Vessels in Major Salivary Glands of the Rat

Aiyama

Kikuchi

Takada

et al. 2011

Okajimas Folia Anat. Jpn.

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Section: Introductionmentioning

confidence: 98%

“…However, their lymphatic vessel lumens are constitutively distended compared with the irregularly shaped, collapsed lumens of lymphatic vessels often found in wild-type mice (Huang et al, 2006). The alteration in the shape of lymphatic vessel lumens is most prominent in the liver and intestine (Huang et al, 2006). High molecular weight FITC-dextran (average MW 2,000,000) injected subcutaneously in the tail of Crsbp1 -/-mice is cleared from the tail more rapidly than in wild-type mice (Huang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…The alteration in the shape of lymphatic vessel lumens is most prominent in the liver and intestine (Huang et al, 2006). High molecular weight FITC-dextran (average MW 2,000,000) injected subcutaneously in the tail of Crsbp1 -/-mice is cleared from the tail more rapidly than in wild-type mice (Huang et al, 2006). Since high molecular weight FITC-dextran can only be cleared via lymphatic drainage, we reasoned that Crsbp1 -/-mice might have a constitutive increase in the transit of large molecules, fluids and cells from the interstitial space into the lumen of lymphatic capillaries, a process we call interstitiallymphatic transit (Huang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…High molecular weight FITC-dextran (average MW 2,000,000) injected subcutaneously in the tail of Crsbp1 -/-mice is cleared from the tail more rapidly than in wild-type mice (Huang et al, 2006). Since high molecular weight FITC-dextran can only be cleared via lymphatic drainage, we reasoned that Crsbp1 -/-mice might have a constitutive increase in the transit of large molecules, fluids and cells from the interstitial space into the lumen of lymphatic capillaries, a process we call interstitiallymphatic transit (Huang et al, 2006). To further define the role of CRSBP-1 in the regulation of interstitial-lymphatic transit, FITCdextran was co-injected with and without PDGF-BB, a putative physiological CRSBP-1 ligand, into tails of wild-type mice.…”

Section: Introductionmentioning

confidence: 99%

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CRSBP-1/LYVE-1 ligands disrupt lymphatic intercellular adhesion by inducing tyrosine phosphorylation and internalization of VE-cadherin

Hou

Liu

Tsai

et al. 2011

Journal of Cell Science

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Cell-surface retention sequence (CRS) binding protein (CRSBP-1) is a membrane glycoprotein identified by its ability to bind PDGF-BB and VEGF-A via their CRS motifs (clusters of basic amino acid residues). CRSBP-1 is identical to LYVE-1 and exhibits dual ligand (CRS-containing proteins and hyaluronic acid) binding activity, suggesting the importance of CRSBP-1 ligands in lymphatic function. Here, we show that CRSBP-1 ligands induce disruption of VE-cadherin-mediated intercellular adhesion and opening of intercellular junctions in lymphatic endothelial cell (LEC) monolayers as determined by immunofluorescence microscopy and Transwell permeability assay. This occurs by interaction with CRSBP-1 in the CRSBP-1–PDGFβR–β-catenin complex, resulting in tyrosine phosphorylation of the complex, dissociation of β-catenin and p120-catenin from VE-cadherin, and internalization of VE-cadherin. Pretreatment of LECs with a PDGFβR kinase inhibitor abolishes ligand-stimulated tyrosine phosphorylation of VE-cadherin, halts the ligand-induced disruption of VE-cadherin intercellular adhesion and blocks the ligand-induced opening of intercellular junctions. These CRSBP-1 ligands also induce opening of lymphatic intercellular junctions that respond to PDGFβR kinase inhibitor in wild-type mice (but not in Crsbp1-null mice) as evidenced by increased transit of injected FITC–dextran and induced edema fluid from the interstitial space into lymphatic vessels. These results disclose a novel mechanism involved in the opening of lymphatic intercellular junctions.

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Lymphatic development in mouse small intestine

Kim

Sung

Koh

2007

Developmental Dynamics

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Lymphatic vessels in the small intestine serve as essential conduits for the absorption and transport of lipids from the intestine to the thoracic duct. Although the morphology and function of the intestinal lymphatic vasculature are well known, little is known about the embryonic development of these vessels. In this study, we examined development of lymphatic and blood vasculatures in the intestinal tube during mouse embryonic development by immunostaining with recently discovered molecular markers for lymphatic endothelial cells: LYVE-1, VEGFR3, Prox-1, and podoplanin. Immature lymphatics became detectable in mesentery, but not in intestinal tube, around E13.5-E14.5, while organized lymphatic vessel plexuses and capillaries were observed in intestinal tube and villi around E17.5. These lymphatic plexuses and capillaries in the intestinal tube appeared to be formed through an active branching process associated with activation of VEGFR3 and involvement of LYVE-1؉ macrophages. Our data also reveal that the lymphatic vessels in the intestinal tube, unlike the blood vessels, have not originated from the mesoderm of intestine. All lymphatic vessels in the intestinal tube originated by extension of mesenteric lymphatic vessels through an active branching process. Although the formation of lymphatic vessels follows the formation of blood vessels in the intestine, a mature lymphatic vasculature is formed before birth. Together, our study reveals the temporal and spatial windows of intestinal lymphatic development during embryonic development in mouse. Developmental Dynamics 236:2020 -2025, 2007.

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CRSBP‐1/LYVE‐l‐null mice exhibit identifiable morphological and functional alterations of lymphatic capillary vessels

Cited by 35 publications

References 31 publications

Immunohistochemical Study of the Lymphatic Vessels in Major Salivary Glands of the Rat

Immunohistochemical Study of the Lymphatic Vessels in Major Salivary Glands of the Rat

CRSBP-1/LYVE-1 ligands disrupt lymphatic intercellular adhesion by inducing tyrosine phosphorylation and internalization of VE-cadherin

Lymphatic development in mouse small intestine

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