CRPC Membrane-Camouflaged, Biomimetic Nanosystem for Overcoming Castration-Resistant Prostate Cancer by Cellular Vehicle-Aided Tumor Targeting

Abstract

Castration-resistant prostate cancer (CRPC) is the most common malignant tumor of the male urinary system. Nanodrug delivery systems (NDDS) have been widely applied in drug delivery for tumor therapy; however, nanotherapeutics encounter various biological barriers that prevent successful accumulation of drugs, specifically at diseased sites. Therefore, there is an urgent need to develop a CRPC-targeting nanocomposite with fine biocompatibility for penetrating various biological barriers, delivering sufficient drugs to the targeting site and improving therapeutic efficiency. In this work, CRPC cell membranes were firstly adapted as biomimetic vectors for the encapsulating PEG−PLGA polymer containing the chemotherapy drug docetaxel (DTX). The CRPC membrane-camouflaged nanoparticles can easily escape early recognition by the immune system, penetrate the extracellular barrier, and evade clearance by the circulatory system. In addition to the characteristics of traditional nanoparticles, the CRPC cell membrane contains an arsenal of highly specific homotypic moieties that can be used to recognize the same cancer cell types and increase the targeted drug delivery of DTX. In vivo fluorescence and radionuclide dual-model imaging were fulfilled by decorating the biomimetic nanosystem with near-infrared dye and isotope, which validated the homotypic targeting property offered by the CRPC cell membrane coating. Importantly, remarkably improved therapeutic efficacy was achieved in a mice model bearing CRPC tumors. This homologous cell membrane enabled an efficient drug delivery strategy and enlightened a new pathway for the clinical application of tumor chemotherapy drugs in the future.

Morphologically transformable peptide nanocarriers coloaded with doxorubicin and curcumin inhibit the growth and metastasis of hepatocellular carcinoma

“…As chemotherapeutic drugs are readily eliminated by tumor cells [ 53 ], it is crucial to prolong drug retention within tumor cells. After 72 h of coincubation with DC, DC/Pep2 and DC/Pep1, confocal microscopy detected the red fluorescence of DOX and the green fluorescence of CUR in HepG2 cells.…”

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

“…Recently, two differently sized FL-SiO 2 NPs, FL-SiO 2 NPs-0 nm and FL-SiO 2 NPs-150 nm, were prepared using surfactant CTAB as a structure-directing agent, with TEOS used as a silica precursor. The carbon dots formed during the calcination process in preparation of FL-SiO 2 NPs produce the stable and strong fluorescence which is essential for in vivo imagining of nanoparticles as delivery vehicles in biological systems [ 48 , 49 , 50 ]. Methoxyfenozide was loaded into FL-SiO 2 NPs by simple immersion in a CH 3 OH solution by magnetically stirring at room temperature.…”

Understanding and targeting resistance mechanisms in cancer

“…reported that the uptake of membrane‐coated nanoparticles in human breast cancer MDA‐MB‐435 cells was increased by 20‐fold compared to naked nanoparticles. 102 Many studies have demonstrated the selectivity and biosafety of cancer cell membrane‐based biomimetic nanoparticles using in vitro and in vivo MDR cancer models, 103 , 104 , 105 suggesting a novel drug delivery strategy to improve therapeutic efficacy for MDR cancer.…”