Crowding-induced membrane remodeling: Interplay of membrane tension, polymer density, architecture

Kandy, Sreeja Kutti; Radhakrishnan, Ravi

doi:10.1016/j.bpj.2022.05.031

Cited by 8 publications

(2 citation statements)

References 57 publications

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“…A consequence of ASL dehydration would be concentrating the secretome. The crowding of surface and membrane-attached macromolecules may affect membrane shaping, as well as protein-protein and protein-lipid interactions (Kutti Kandy & Radhakrishnan, 2022). For instance, failure of mucociliary clearance in CF is due to abnormal mucus biophysical properties (Okuda et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Apical dehydration impairs the cystic fibrosis airway epithelium barrier via aβ1-integrin/YAP1 pathway

Simonin,

Tomba,

Mercier

et al. 2024

Life Sci. Alliance

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Defective hydration of airway surface mucosa is associated with lung infection in cystic fibrosis (CF), partly caused by disruption of the epithelial barrier integrity. Although rehydration of the CF airway surface liquid (ASL) alleviates epithelium vulnerability to infection by junctional protein expression, the mechanisms linking ASL to barrier integrity are unknown. We show here the strong degradation of YAP1 and TAZ proteins in well-polarized CF human airway epithelial cells (HAECs), a process that was prevented by ASL rehydration. Conditional silencing ofYAP1in rehydrated CF HAECs indicated that YAP1 expression was necessary for the maintenance of junctional complexes. A higher plasma membrane tension in CF HAECs reduced endocytosis, concurrent with the maintenance of activeβ1-integrin ectopically located at the apical membrane. Pharmacological inhibition ofβ1-integrin accumulation restored YAP1 expression in CF HAECs. These results indicate that dehydration of the CF ASL affects epithelial plasma membrane tension, resulting in ectopic activation of aβ1-integrin/YAP1 signaling pathway associated with degradation of junctional proteins.

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Section: Discussionmentioning

confidence: 99%

Apical dehydration impairs the cystic fibrosis airway epithelium barrier via aβ1-integrin/YAP1 pathway

Simonin,

Tomba,

Mercier

et al. 2024

Life Sci. Alliance

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show abstract

“…Cell membrane adhesion, which can be seen from a biophysical perspective as pinning of membranes (such as membrane-cytoskeleton linker proteins), has also been implicated in promoting curvature generation ( Kutty Kandy and Radhakrishnan, 2019 ). Another factor to consider is the glycocalyx-induced membrane remodeling ( Kutty Kandy and Radhakrishnan, 2022 ). We have also not undertaken in vivo experimental studies for the signature proteins identified by our analysis.…”

Section: Discussionmentioning

confidence: 99%

Data driven and biophysical insights into the regulation of trafficking vesicles by extracellular matrix stiffness

et al. 2022

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Free energy calculations for membrane morphological transformations and insights to physical biology and oncology

Parihar,

Ko,

Bradley

et al. 2024

Methods in Enzymology

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Crowding-induced membrane remodeling: Interplay of membrane tension, polymer density, architecture

Cited by 8 publications

References 57 publications

Apical dehydration impairs the cystic fibrosis airway epithelium barrier via aβ1-integrin/YAP1 pathway

Apical dehydration impairs the cystic fibrosis airway epithelium barrier via aβ1-integrin/YAP1 pathway

Data driven and biophysical insights into the regulation of trafficking vesicles by extracellular matrix stiffness

Free energy calculations for membrane morphological transformations and insights to physical biology and oncology

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