Crosstalk of Nataxazole Pathway with Chorismate‐Derived Ionophore Biosynthesis Pathways in <i>Streptomyces</i> sp. Tü 6176

Cano-Prieto, Carolina; Losada, Armando A.; Braña, Alfredo F.; Méndez, Cármen; Salas, José A.; Olano, Carlos

doi:10.1002/cbic.201500261

Cited by 11 publications

(17 citation statements)

References 30 publications

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“…This co-regulatory mechanism involves a metabolic intermediate that is transformed through enzymatic activity into a signal regulating gene expression. In contrast to previously described co-regulatory mechanisms involving a metabolic intermediate (Vingadassalon et al, 2015; Cano-Prieto et al, 2015), the metabolic intermediate responsible for coordination of DAPG and pyoluteorin production is not shared by the two biosynthetic pathways. Instead, PG is an intermediate in DAPG biosynthesis and a precursor for formation of signaling molecules that activate expression of pyoluteorin biosynthetic genes (Figure 8).…”

Section: Discussioncontrasting

confidence: 91%

“…The biosynthesis of different secondary metabolites by a microorganism is often coordinated (Bosello et al, 2011; Tsunematsu et al, 2013; Hidalgo et al, 2014; Vingadassalon et al, 2015; Cano-Prieto et al, 2015; Cui et al, 2016). Co-regulation of different secondary metabolites is thought to be selected during microbial evolution because it confers competitive advantages to the producing organism in microbial interactions (Challis and Hopwood, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…The first type of co-regulation involves one or more intermediates that are shared by different biosynthetic pathways (Vingadassalon et al, 2015; Cano-Prieto et al, 2015). The second type results when a single enzyme is shared by different pathways (Lazos et al, 2010; Tsunematsu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens

Yan

Philmus

Chang

et al. 2017

eLife

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Metabolic co-regulation between biosynthetic pathways for secondary metabolites is common in microbes and can play an important role in microbial interactions. Here, we describe a novel mechanism of metabolic co-regulation in which an intermediate in one pathway is converted into signals that activate a second pathway. Our study focused on the co-regulation of 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin, two antimicrobial metabolites produced by the soil bacterium Pseudomonas protegens. We show that an intermediate in DAPG biosynthesis, phloroglucinol, is transformed by a halogenase encoded in the pyoluteorin gene cluster into mono- and di-chlorinated phloroglucinols. The chlorinated phloroglucinols function as intra- and inter-cellular signals that induce the expression of pyoluteorin biosynthetic genes, pyoluteorin production, and pyoluteorin-mediated inhibition of the plant-pathogenic bacterium Erwinia amylovora. This metabolic co-regulation provides a strategy for P. protegens to optimize the deployment of secondary metabolites with distinct roles in cooperative and competitive microbial interactions.DOI: http://dx.doi.org/10.7554/eLife.22835.001

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Section: Discussioncontrasting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens

Yan

Philmus

Chang

et al. 2017

eLife

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“…MBT76. Similar functional crosstalk was also found during the biosynthesis of enterobactin and other secondary metabolites benzoxazoles and caboxamycin in other Streptomyces species (Cano-Prieto et al, 2015; Losada et al, 2017). The antiSMASH analysis showed a large number of PKS and NRPS gene cluster distributed in the genome of NEAU-S7GS2, whether the similar crosstalk exists in NEAU-S7GS2 merits further investigation.…”

Section: Discussionsupporting

confidence: 68%

Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Liu

Yan

et al. 2019

Front. Microbiol.

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Diseases caused by Sclerotinia sclerotiorum have caused severe losses of many economically important crops worldwide. Due to the long-term persistence of sclerotia in soil and the production of air-borne ascospores, synthetic fungicides play limited roles in controlling the diseases. The application of antagonistic microorganisms can effectively reduce the number of sclerotia and eventually eradicate S. sclerotiorum from soil, and therefore considerable interest has been focused on biological control. Streptomyces sp. NEAU-S7GS2 was isolated from the root of Glycine max and its rhizosphere soil. It showed significant inhibitory activity against the mycelial growth of S. sclerotiorum (99.1%) and completely inhibited sclerotia germination. Compared to the control, in the pot experiment the application of NEAU-S7GS2 not only demonstrated excellent potential to control sclerotinia stem rot of soybean with 77 and 38% decrease in disease incidence and disease index, respectively, but could promote the growth of soybean. The light microscopy and scanning electron microscopy showed that co-culture of NEAU-S7GS2 with S. sclerotiorum on potato dextrose agar could lead to contorted and fragmented mycelia of S. sclerotiorum, which was associated with the secretion of hydrolytic glucanase and cellulase and the production of active secondary metabolites by NEAU-S7GS2. The plant growth promoting activity of NEAU-S7GS2 was related to the solubilization of inorganic phosphate, and production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase and indole acetic acid (IAA). To further explore the plant growth promoting and antifungal mechanisms, the complete genome of strain NEAU-S7GS2 was sequenced. Several genes associated with ammonia assimilation, phosphate solubilization and IAA synthesis, together with genes encoding ACC deaminase, glucanase and α-amylase, were identified. AntiSMASH analysis led to the identification of four gene clusters responsible for the biosynthesis of siderophores including desferrioxamine B and enterobactin. Moreover, the biosynthetic gene clusters of lydicamycins, phenazines, and a glycosylated polyol macrolide showing 88% gene similarity to PM100117/PM100118 were identified. These results suggested that strain NEAU-S7GS2 may be a potential biocontrol agent and biofertilizer used in agriculture.

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“…NTK937 genome contains several non‐ribosomal peptide synthase (NRPS)‐harbouring clusters (Olano et al ., ), one of which, based on its similarity to the enterobactin cluster in Streptomyces sp. Tü6176 (Cano‐Prieto et al ., ), might be responsible for the production of this siderophore. The presence of this cluster in caboxamycin producer Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Caboxamycin biosynthesis pathway and identification of novel benzoxazoles produced by cross‐talk in Streptomyces sp. NTK 937

Losada

Cano-Prieto

García-Salcedo

et al. 2017

Microbial Biotechnology

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Summary Streptomyces sp. NTK937, producer of benzoxazole antibiotic caboxamycin, produces in addition a methyl ester derivative, O‐methylcaboxamycin. Caboxamycin cluster, comprising one regulatory and nine structural genes, has been delimited, and each gene has been individually inactivated to demonstrate its role in the biosynthetic process. The O‐methyltransferase potentially responsible for O‐methylcaboxamycin synthesis would reside outside this cluster. Five of the genes, cbxR, cbxA, cbxB, cbxD and cbxE, encoding a SARP transcriptional regulator, salicylate synthase, 3‐oxoacyl‐ACP‐synthase, ACP and amidohydrolase, respectively, have been found to be essential for caboxamycin biosynthesis. The remaining five structural genes were found to have paralogues distributed throughout the genome, capable of partaking in the process when their cluster homologue is inactivated. Two of such paralogues, cbxC’ and cbxI’, coding an AMP‐dependent synthetase‐ligase and an anthranilate synthase, respectively, have been identified. However, the other three genes might simultaneously have more than one paralogue, given that cbxF (DAHP synthase), cbxG (2,3‐dihydro‐2,3‐dihydroxybenzoate dehydrogenase) and cbxH (isochorismatase) have three, three and five putative paralogue genes, respectively, of similar function within the genome. As a result of genetic manipulation, a novel benzoxazole (3′‐hydroxycaboxamycin) has been identified in the salicylate synthase‐deficient mutant strain ΔcbxA. 3′‐hydroxycaboxamycin derives from the cross‐talk between the caboxamycin and enterobactin pathways.

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Crosstalk of Nataxazole Pathway with Chorismate‐Derived Ionophore Biosynthesis Pathways in Streptomyces sp. Tü 6176

Cited by 11 publications

References 30 publications

Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens

Novel mechanism of metabolic co-regulation coordinates the biosynthesis of secondary metabolites in Pseudomonas protegens

Antifungal, Plant Growth-Promoting, and Genomic Properties of an Endophytic Actinobacterium Streptomyces sp. NEAU-S7GS2

Caboxamycin biosynthesis pathway and identification of novel benzoxazoles produced by cross‐talk in Streptomyces sp. NTK 937

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