Crosstalk During Fruit Ripening and Stress Response Among Abscisic Acid, Calcium-Dependent Protein Kinase and Phenylpropanoid

Vighi, Isabel Lopes; Crizel, Rosane Lopes; Perin, Ellen Cristina; Rombaldi, César Valmor; Galli, Vanessa

doi:10.1080/07352689.2019.1602959

Cited by 34 publications

(16 citation statements)

References 138 publications

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“…Indeed, regulation of transcription and transcription factor activity appeared as an important biological process and molecular function categories, respectively (see Figure 4). As regards to the transcriptional control, several genes of the MAPK signaling pathway also modify their transcript accumulation level during pod maturation, which confirms the essential role that phosphorylation plays as a regulatory mechanism of gene expression [51,52].…”

Section: Transcriptome Analyses Reveal Pod Maturation Specific Genes supporting

confidence: 52%

Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)

Gómez-Martín

Capel

González

et al. 2020

Plants

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Pod maturation of common bean relies upon complex gene expression changes, which in turn are crucial for seed formation and dispersal. Hence, dissecting the transcriptional regulation of pod maturation would be of great significance for breeding programs. In this study, a comprehensive characterization of expression changes has been performed in two common bean cultivars (ancient and modern) by analyzing the transcriptomes of five developmental pod stages, from fruit setting to maturation. RNA-seq analysis allowed for the identification of key genes shared by both accessions, which in turn were homologous to known Arabidopsis maturation genes and furthermore showed a similar expression pattern along the maturation process. Gene-expression changes suggested a role in promoting an accelerated breakdown of photosynthetic and ribosomal machinery associated with chlorophyll degradation and early activation of alpha-linolenic acid metabolism. A further study of transcription factors and their DNA binding sites revealed three candidate genes whose functions may play a dominant role in regulating pod maturation. Altogether, this research identifies the first maturation gene set reported in common bean so far and contributes to a better understanding of the dynamic mechanisms of pod maturation, providing potentially useful information for genomic-assisted breeding of common bean yield and pod quality attributes.Plants 2020, 9, 545 2 of 20 metabolites such as carotenoids and anthocyanins [7][8][9][10][11]. Likewise, jasmonate promotes senescence and plays an important role in chlorophyll degradation [12,13], which is synthesized from alpha-linolenic acid [14,15], suggesting that the accumulation of this acid during maturation is responsible for stimulating chlorophyll loss [16]. In addition, the increased accumulation of antioxidant and monoterpene is also an important feature of the fruit maturation process [17][18][19].Like most species of the Fabaceae (also named as Leguminosae) family, common bean (Phaseolus vulgaris L.) produces dry fruits in the form of a pod, which is a photosynthetically active organ that encloses the developing seeds and protects them from pests and pathogens. Indeed, photosynthetically active pod tissue contributes to fuel seed growth with assimilates and nutrients [20]. As in most angiosperms, significant genetic, biochemical, and physiological changes take place during fruit maturation, which confers the functional and morphological properties of this plant organ. Unlike members of the Brassicaceae family that produce pods (siliques) derived from two fused carpels, legume pods are developed from a single carpel that emerges from the gynoecium after ovule fertilization. Subsequently, pods attain their maximum size through a growth phase caused by an active cell division and later cell expansion [21]. During the maturation phase, carbohydrate metabolism and amino acid biosynthesis are increased in pods, leading to the accumulation of storage compounds, mainly starch, total soluble amino acids, an...

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Section: Transcriptome Analyses Reveal Pod Maturation Specific Genes supporting

confidence: 52%

Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)

Gómez-Martín

Capel

González

et al. 2020

Plants

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“…Therefore, understanding the mechanisms underlying this process is essential to achieve optimal fruit quality as well as long shelf life. Transcriptomic, metabolomic, and gene silencing studies have shown that this process in non-climacteric fruits such as strawberry is ABA-dependent 56 , and triggered by a signaling cascade involving calcium (Ca 2+ ) and calcium-dependent protein kinases (CDPKs) 15 .Increased expression of FaCDPK3 here indicates a possible role during strawberry maturation. However, further studies are necessary to address ABA-dependence of this process.…”

Section: Discussionmentioning

confidence: 83%

“…ABA mainly functions as a regulator of plant water balance and osmotic stress tolerance 65 . CDPK isoforms were also shown to differ in their Ca 2+ affinity, and calcium influx generated by ABA is one of the determinants of the induction of CDPKs 15 . As an example, Zhang H. et al 22 performed an analysis of the transcripts of 21 BnaCDPK genes in canola seedlings following different treatments under abiotic stress conditions, and found that different CDPK genes may participate in the signaling processes of a single stress factor, and a single CDPK gene likely plays a role in multiple stress responses.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, FaCDPK4 and FaCDPK11 were found to be involved in drought stress tolerance, and utilize ABA as the signaling molecule to increase the content of phenolics, anthocyanins, ascorbic acid, and sugar compounds. The crosstalk between CDPKs, ABA, phenylpropanoids and sugar was recently reviewed by Vighi et al 15 , and the influence of ABA in the ascorbic acid metabolism of strawberry fruits was discussed by Li et al 71 .…”

Section: Discussionmentioning

confidence: 99%

“…Stress response involves the production of reactive oxygen species (ROS), and may induce the flow of calcium into the cell. Both signaling molecules induce downstream signaling events that result in the activation of Ca 2+ sensor proteins including CDPK as well as the ROS-producing enzyme andr espiratory oxidase homolog proteins (RBOH) 15 .…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa

Crizel¹,

Perin

Vighi

et al. 2020

Sci Rep

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Calcium-dependent protein kinases (CDPKs) are encoded by a large gene family and play important roles against biotic and abiotic stresses and in plant growth and development. To date, little is known about the CDPK genes in strawberry (Fragaria x ananassa). In this study, analysis of Fragaria x ananassa CDPK gene family was performed, including gene structures, phylogeny, interactome and expression profiles. Nine new CDPK genes in Fragaria x ananassa were identified based on RNA-seq data. These identified strawberry FaCDPK genes were classified into four main groups, based on the phylogenetic analysis and structural features. FaCDPK genes were differentially expressed during fruit development and ripening, as well as in response to abiotic stress (salt and drought), and hormone (abscisic acid) treatment. In addition, the interaction network analysis pointed out proteins involved in the ABA-dependent response to plant stress via Ca 2+ signaling, especially RBOHs. To our knowledge, this is the first report on CDPK families in Fragaria x ananassa, and it will provide valuable information for development of biofortified fruits and stress tolerant plants. Plants are constantly exposed to stress conditions, such as drought, low or high temperature, and high salinity 1. Plants adapt to these conditions by capturing external signals and modulating their responses using complex mechanisms. These mechanisms involve the perception of the stimulus and subsequent signal transduction, which lead to the activation of various chemical, molecular, biochemical, and physiologic changes, improving plant plasticity 2. The calcium ion (Ca 2+) plays central roles in the regulation of different physiological processes in plants as an important secondary messenger. Transient changes in the cytoplasmic concentration of Ca 2+ are detected by several types of sensor proteins that initiate rapid signal transduction processes by triggering cascades of phosphorylation events. Several major classes of Ca 2+ binding proteins have been characterized in plants, including Ca 2+-dependent protein kinases (CDPK), calmodulin (CaM), CAM-like proteins (CML), and calcineurin B-like proteins (CBL) 3-6. Among these, CDPK constitutes a large calcium-sensing family only found in plants, protists, oomycetes, and green algae, and not in animals and fungi 7. CDPK structures include four domains: N-terminal, self-regulatory/autoinhibitory, serine/threonine kinase, and finally calmodulin-like regulatory domains (CaM-LD) 8. The CaM-LD domain contains three or four EFhand Ca 2+-binding motifs that recognize distinct Ca 2+ signatures with variable affinities. The C-terminal lobe of the CaM-LD binds Ca 2+ with high affinity. At low Ca 2+ levels, the structure is stabilized by the interaction of this lobe with the auto-inhibitory region of the protein. Otherwise, a conformational change induced by the binding of Ca 2+ to the low-affinity N-terminal lobe of CaM-LD results in the release of the auto-inhibition 9,10. CDPKs are involved in stress signaling, hormone respon...

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Role of Phytomelatonin in Promoting Ion Homeostasis During Salt Stress

El-Badri,

Batool,

Mohamed

et al. 2023

Plant in Challenging Environments

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Crosstalk During Fruit Ripening and Stress Response Among Abscisic Acid, Calcium-Dependent Protein Kinase and Phenylpropanoid

Cited by 34 publications

References 138 publications

Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)

Transcriptional Dynamics and Candidate Genes Involved in Pod Maturation of Common Bean (Phaseolus vulgaris L.)

Genome-wide identification, and characterization of the CDPK gene family reveal their involvement in abiotic stress response in Fragaria x ananassa

Role of Phytomelatonin in Promoting Ion Homeostasis During Salt Stress

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