Crosstalk between uterine serpin (SERPINA14) and pregnancy-associated glycoproteins at the fetal-maternal interface in pregnant dairy heifers experimentally infected with Neospora caninum

Serrano-Pérez, B.; Hansen, Peter J.; Mur-Novales, R.; García-Ispierto, Irina; Sousa, Noelita Melo de; Beckers, Jean‐François; Almerı́a, S.; López‐Gatius, F.

doi:10.1016/j.theriogenology.2016.03.003

Cited by 16 publications

(14 citation statements)

References 47 publications

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“…Total RNA was extracted as described in Serrano‐Pérez et al. (). Messenger RNA expression was determined by real‐time RT‐PCR on four target genes: IFN γ, IL4, IL10 and SERPINA14 .…”

Section: Methodsmentioning

confidence: 99%

“…SERPINA14 gene expression was determined by real‐time PCR in intercaruncular, placental and luteal samples (R2: 0.999, slope: −3.434) as described in Serrano‐Pérez et al. (). The gene β‐actin ( ACTB ) was used as housekeeping gene (R2: 0.992, slope: −3.195) to normalize levels of SERPINA14 gene expression.…”

Section: Methodsmentioning

confidence: 99%

“…Intercaruncular, UTLN, placental and luteal tissue samples were managed as described in Almería et al (2016). Total RNA was extracted as described in Serrano-Pérez et al (2016). Messenger RNA expression was determined by real-time RT-PCR on four target genes: IFNγ, IL4, IL10 and SERPINA14.…”

Section: Methodsmentioning

confidence: 99%

“…Cytokine gene expression (IFNγ, IL4, IL10) in UTLN, placental and luteal samples was determined as described in Almería et al (2016). SERPINA14 gene expression was determined by real-time PCR in intercaruncular, placental and luteal samples (R2: 0.999, slope: −3.434) as described in Serrano-Pérez et al (2016). The gene β-actin (ACTB) was used as housekeeping gene (R2: 0.992, slope: −3.195) to normalize levels of SERPINA14 gene expression.…”

Section: Methodsmentioning

confidence: 99%

“…Pregnancy‐associated glycoproteins I (PAG‐I) and II (PAG‐II), indicators of foetal–placental health, coexist in the ruminant trophectoderm (Garbayo, Serrano, & Lopez‐Gatius, ). In a recent study, expression patterns of the genes SERPINA14 , PAG1 and PAG2 at the foetal–maternal interface were investigated in dairy heifers experimentally infected with N. caninum (Serrano‐Pérez et al., ). In aborting infected dams, SERPINA14 expression was significantly reduced and a negative relationship was observed between N. caninum antibody titres and SERPINA14 or PAG expression in all infected animals (Serrano‐Pérez et al., ).…”

Section: Introductionmentioning

confidence: 99%

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Uterine serpin (SERPINA 14) correlates negatively with cytokine production at the foetal–maternal interface but not in the corpus luteum in pregnant dairy heifers experimentally infected with Neospora caninum

Serrano-Pérez

Almerı́a

Mur-Novales

et al. 2017

Reprod Domestic Animals

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This study examines gene expression patterns in dairy heifers experimentally infected with N. caninum during on Day 110 of pregnancy with live foetuses at euthanasia, 42 days later. The study population was constituted of four non-infected controls and three infected dams. Gene expression was determined on gamma interferon (IFNγ), (Th1 pro-inflammatory cytokine), interleukin-4 (IL4) (Th2 pro-gestation cytokine) or interleukin-10 (IL10) (T regulatory cytokine) and the serine peptidase inhibitor SERPINA14 in intercaruncular, placental, uterine lymph node (UTLN) and luteal tissue samples. Intercaruncular SERPINA14 expression was negatively correlated with IFNγ expression in cotyledon samples and with IL4 expression in UTLN. No relationships were detected between cytokine gene expression at the foetal-maternal interface and SERPINA14 expression in the luteal samples. Our findings suggest that gene expression of the uterine serpin SERPINA14 correlates negatively with the expression of Th1 and Th2 cytokines at the foetal-maternal interface but not in the corpus luteum.

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“…Total RNA was extracted as described in Serrano‐Pérez et al. (). Messenger RNA expression was determined by real‐time RT‐PCR on four target genes: IFN γ, IL4, IL10 and SERPINA14 .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%