Crosstalk Between the Sporophyte and the Gametophyte During Anther and Ovule Development in Angiosperms

Lora, Jorge; Hormaza, J. I.

doi:10.1007/124_2020_50

Cited by 2 publications

(4 citation statements)

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“…During the last step of MiMC development and meiosis, there is intense crosstalk between the MiMC and the surrounding tapetum ( Lora and Hormaza, 2020 ). Diploid MiMCs are surrounded by callose, an important polysaccharide that acts as a physical molecular filter ( Tucker et al, 2001 ) and forms a tetrad with haploid microspores through meiosis.…”

Section: Discussionmentioning

confidence: 99%

“…After microspores are released from the tetrad, they mature as individual pollen grains by asymmetric division (mitosis I). They start to develop the inner (intine) and outer (exine) pectocellulosic layers of their cell walls, in which the tapetum plays an essential role in the deposition of components for exine formation ( Lora and Hormaza, 2020 ). In Limonium sp.…”

Section: Discussionmentioning

confidence: 99%

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Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species

2022

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Seedlessness is one of the most important agronomic traits in mandarins on the fresh fruit market. Creation of triploid plants is an important breeding strategy for development of new commercial varieties of seedless citrus. To this end, one strategy is to perform sexual hybridizations, with tetraploid genotypes as male parents. However, while seed development has been widely studied in citrus, knowledge of key steps such as microsporogenesis and microgametogenesis, is scarce, especially in polyploids. Therefore, we performed a study on the effect of ploidy level on pollen development by including diploid and tetraploid (double diploid) genotypes with different degrees of pollen performance. A comprehensive study on the pollen ontogeny of diploid and doubled diploid “Sanguinelli” blood orange and “Clemenules” clementine was performed, with focus on pollen grain germination in vitro and in planta, morphology of mature pollen grains by scanning electron microscopy (SEM), cytochemical characterization of carbohydrates by periodic acid–Shiff staining, and specific cell wall components revealed by immunolocalization. During microsporogenesis, the main difference between diploid and doubled diploid genotypes was cell area, which was larger in doubled diploid genotypes. However, after increase in size and vacuolization of microspores, but before mitosis I, doubled diploid “Clemenules” clementine showed drastic differences in shape, cell area, and starch hydrolysis, which resulted in shrinkage of pollen grains. The loss of fertility in doubled diploid “Clemenules” clementine is mainly due to lack of carbohydrate accumulation in pollen during microgametogenesis, especially starch content, which led to pollen grain abortion. All these changes make the pollen of this genotype unviable and very difficult to use as a male parent in sexual hybridization with the objective of recovering large progenies of triploid hybrids.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species

2022

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“…The production of gametes proceeds in two steps: sporogenesis, followed by gametogenesis. In the anther, hypodermal archesporial cells divide to produce outer primary parietal cells which become somatic tissues, and inner primary sporogenous cells which will then divide mitotically to become microspore mother cells that undergo meiosis (Lora and Hormaza, 2021). Ovule initiation arises from the medial meristem tissue within the carpel.…”

Section: Meiosis In Plantsmentioning

confidence: 99%

“…Immediately following ovule initiation three distinct regions arise: funiculus, chalaza, and nucellus. The nucellus gives rise to the megaspore mother cell which undergoes meiosis to generate four megaspores, three of which degrade leaving a single functional megaspore (Lora and Hormaza, 2021).…”

Section: Meiosis In Plantsmentioning

confidence: 99%

Overcoming roadblocks for in vitro nurseries in plants: induction of meiosis

et al. 2023

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Efforts to increase genetic gains in breeding programs of flowering plants depend on making genetic crosses. Time to flowering, which can take months to decades depending on the species, can be a limiting factor in such breeding programs. It has been proposed that the rate of genetic gain can be increased by reducing the time between generations by circumventing flowering through the in vitro induction of meiosis. In this review, we assess technologies and approaches that may offer a path towards meiosis induction, the largest current bottleneck for in vitro plant breeding. Studies in non-plant, eukaryotic organisms indicate that the in vitro switch from mitotic cell division to meiosis is inefficient and occurs at very low rates. Yet, this has been achieved with mammalian cells by the manipulation of a limited number of genes. Therefore, to experimentally identify factors that switch mitosis to meiosis in plants, it is necessary to develop a high-throughput system to evaluate a large number of candidate genes and treatments, each using large numbers of cells, few of which may gain the ability to induce meiosis.

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Crosstalk Between the Sporophyte and the Gametophyte During Anther and Ovule Development in Angiosperms

Cited by 2 publications

References 106 publications

Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species

Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species

Overcoming roadblocks for in vitro nurseries in plants: induction of meiosis

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