Crosstalk between miRNAs and their regulated genes network in stroke

Abstract

In recent years, more and more studies focus on the roles of genes or miRNAs in stroke. However, the molecular mechanism connecting miRNAs and their targetgenes remains unclear. The aim of this study was to determine the differential regulation and correlations between miRNAs and their targetgenes in human stroke. Stroke-related miRNAs were obtained from the Human MicroRNA Disease Database (HMDD) and their targetgenes were generated from three independent sources. Kappa score was used to create the network and the functional modules. A total of 11 stroke-related miRNAs were identified from the HMDD and 441 overlapping targetgenes were extracted from the three databases. By network construction and GO analysis, 13 functional modules, 186 biological processes, and 21 pathways were found in the network, of which functional module 8 was the largest module, cellular-related process and phosphate-related process were the most important biological processes, and MAPK signaling pathway was the most significant pathway. In our study, all miRNAs regulate the stroke modular network by their targetgenes. After the validation of miRNAs, we found that miR-605 and miR-181d were highly expressed in the blood of stroke patients which never reported before may supply novel target for treatment.

Global analysis of A-to-I RNA editing reveals association with common disease variants

“…We found an association between plasma HDL levels and editing of pri-mir-27b/mir-605. mir-27b has been linked to progression of atherosclerosis ( Li et al, 2011a ; Zhang et al, 2014b ), and mir-605 is implicated in stroke ( Yuan et al, 2016 ) and hypertension ( Li et al, 2011b ). These data highlight the need to further integrate A-to-I editing data with respect to the short non-coding transcriptome.…”

Inhibiting MicroRNA-503 and MicroRNA-181d with Losartan Ameliorates Diabetic Nephropathy in KKAy Mice

“…Studies have shown that microRNA-181d may regulate the expression of matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinase (TIMP), heat shock protein 70, Bcl-2 family members, mitogen-activated protein kinase (MAPK), and the Notch signaling pathway, among others [34,35,37–39], and miR-181d-5p is the key regulating miRNA of expression of O6-methylguanine-DNA methyltransferase (MGMT) [40,41]. Our previous proteomics study also found differential expression of the predicted target genes of miR-181d, such as heat shock protein 75, GRP75, and GRP78, in the glomeruli of diabetic KKAy mice (in press).…”

Nucleic Acid Therapies for Ischemic Stroke

“…In this respect, nucleic acid therapies targeting microRNA (miRNA) may be attractive because distinct miRNA expression patterns have been found in patients with ischemic stroke with both up-and downregulation of miRNAs as assessed in the blood [140], and also because miRNAs have been implicated in the regulation of factors involved in neuronal cell death as well as reperfusion-associated injury [141][142][143]. For example, miR-215 has been found upregulated in preclinical models of neuronal ischemic injury and overexpression of miR-215 inhibited apoptosis and autophagy in vitro, as well as attenuated the infarct volume and improved functional deficits in a mouse ischemic stroke model [144].…”