Crosstalk between Metabolism and Virulence of<i>Legionella pneumophila</i>

Heuner, Klaus; Eisenreich, Wolfgang

doi:10.1002/9783527682386.ch2

Cited by 3 publications

(4 citation statements)

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“…Indeed, host cell glycogen could be degraded to glucose by the action of the bacterial glucoamylase GamA (19,34). Further supporting the role of glucose as a nutrient for intracellular L. pneumophila, glucose uptake was found to be increased during the late phases of growth (33), and Legionella species-specific differences in their usages of glucose and serine as carbon substrates were suggested recently (35,36). However, the differential transfer of substrates during the different growth phases of L. pneumophila has not yet been directly shown.…”

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confidence: 77%

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

Gillmaier

Schunder

Kutzner

et al. 2016

Journal of Biological Chemistry

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Legionella pneumophila, the causative agent of Legionnaires disease, has a biphasic life cycle with a switch from a replicative to a transmissive phenotype. During the replicative phase, the bacteria grow within host cells in Legionella-containing vacuoles. During the transmissive phenotype and the postexponential (PE) growth phase, the pathogens express virulence factors, become flagellated, and leave the Legionella-containing vacuoles. Using 13 C labeling experiments, we now show that, under in vitro conditions, serine is mainly metabolized during the replicative phase for the biosynthesis of some amino acids and for energy generation. During the PE phase, these carbon fluxes are reduced, and glucose also serves as an additional carbon substrate to feed the biosynthesis of poly-3-hydroxybuyrate (PHB), an essential carbon source for transmissive L. pneumophila. Whole-cell FTIR analysis and comparative isotopologue profiling further reveal that a putative 3-ketothiolase (Lpp1788) and a PHB polymerase (Lpp0650), but not enzymes of the crotonylCoA pathway (Lpp0931-0933) are involved in PHB metabolism during the PE phase. However, the data also reflect that additional bypassing reactions for PHB synthesis exist in agreement with in vivo competition assays using Acanthamoeba castellannii or human macrophage-like U937 cells as host cells. The data suggest that substrate usage and PHB metabolism are coordinated during the life cycle of the pathogen.

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confidence: 77%

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

Gillmaier

Schunder

Kutzner

et al. 2016

Journal of Biological Chemistry

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“…Secreted effector proteins (like AnkB) of the Icm/Dot system are involved in the ubiquitin‐dependent proteolysis by the host proteasome, leading to an increase in free amino acids . The usage of amino acids as a nutrient and carbon source by L. pneumophila during replication within the LCV, as well as their use directly for protein biosynthesis, was recently shown using the method of isotopologue profiling . The amino acids may be imported into the LCV from the host cytosol by different solute carriers (Fig.…”

Section: Host‐adapted Metabolism Of L Pneumophilamentioning

confidence: 99%

“…Some genes ( lpp gene numbers) or protein names are given within the circles. CIT, citrate; Ery‐4P, erythrose‐4‐phosphate; FA, fatty acids; Fru, fructose; Hex‐P, hexosephosphate transport protein; KG, ketoglutarate; Lpp, L. pneumophila Paris; MAL malate; Man, mannose; OAA, oxaloacetate; Pyr, pyruvate; Xyl‐5P, xylulose‐5‐phosphate; reaction 1, serine dehydratase; reaction 2, Glu‐Asp transaminase; reaction 3, PHB synthesis pathway (3‐ketothiolase, acetoacetyl‐CoA‐reductase, PHB polymerase); reaction 4, transketolase (modified from ).…”

Section: Host‐adapted Metabolism Of L Pneumophilamentioning

confidence: 99%

The life stage‐specific pathometabolism of Legionella pneumophila

Eisenreich

Heuner

2016

FEBS Letters

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The genus Legionella belongs to Gram-negative bacteria found ubiquitously in aquatic habitats, where it grows in natural biofilms and replicates intracellularly in various protozoa (amoebae, ciliates). L. pneumophila is known as the causative agent of Legionnaires' disease, since it is also able to replicate in human alveolar macrophages, finally leading to inflammation of the lung and pneumonia. To withstand the degradation by its host cells, a Legionellacontaining vacuole (LCV) is established for intracellular replication, and numerous effector proteins are secreted into the host cytosol using a type four B secretion system (T4BSS). During intracellular replication, Legionella has a biphasic developmental cycle that alternates between a replicative and a transmissive form. New knowledge about the host-adapted and life stagedependent metabolism of intracellular L. pneumophila revealed a bipartite metabolic network with life stage-specific usages of amino acids (e.g. serine), carbohydrates (e.g. glucose) and glycerol as major substrates. These metabolic features are associated with the differentiation of the intracellular bacteria, and thus have an important impact on the virulence of L. pneumophila.

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“…A pathogen is a microorganism such as a bacterium, or fungus (may be yeast) or virus that causes disease in its plant or animal host. The ability of the pathogen to infect is called its pathogenicity which can be expressed by means of their virulence, that is, the relative and quantitative degree of pathogenicity [1]. However, in an attempt to control the infection, a host's immune system can also cause damage to the host itself.…”

Section: Introductionmentioning

confidence: 99%

Bacteria Emerging As an Opportunistic Pathogen

Chahal¹

2021

ijcsrr

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Due to the re-emergence of infectious diseases, the causative pathogenic microorganisms are becoming the major microbiologic public health threat. It seems to be difficult to control the emergence of new and severe bacterial diseases. However, efforts are continuously being made to identify the main cause to prevent the uncontrolled spread of emerging diseases. This review focuses on emerging bacterial diseases and their causative bacteria and the pathway of pathogenesis.

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Crosstalk between Metabolism and Virulence ofLegionella pneumophila

Cited by 3 publications

References 78 publications

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

Growth-related Metabolism of the Carbon Storage Poly-3-hydroxybutyrate in Legionella pneumophila

The life stage‐specific pathometabolism of Legionella pneumophila

Bacteria Emerging As an Opportunistic Pathogen

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