Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by S. agalactiae

Guo, Zhixin; Ma, Yuze; Jia, Zhibo; Wang, Liping; Lu, Xinyue; Chen, Yuhao; Wang, Yanfeng; Hao, Huifang; Yu, Shuixing; Wang, Zhigang

doi:10.1016/j.isci.2023.107884

Cited by 1 publication

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References 63 publications

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“…The RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β signaling pathway plays a crucial role in modulating actin assembly in various cellular types in response to extracellular stimuli [41]. Previous studies showed that S. aureus [9] and S. agalactiae [42] infection induced BMEC actin-cytoskeleton rearrangement through Rho GTPase (RhoA) regulated pathway modulation. The internalization of S. aureus and phosphorylation of GSK-3α (Ser21),and GSK-3β (Ser9) are associated with the PI3K/Akt signaling pathway in bovine endothelial cells [43].…”

Section: Discussionmentioning

confidence: 99%

Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells

Yang,

Hai,

Hou

et al. 2023

IJMS

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Pore-forming toxins (PFTs) exert physiological effects by rearrangement of the host cell cytoskeleton. Staphylococcus aureus-secreted PFTs play an important role in bovine mastitis. In the study, we examined the effects of recombinant Panton–Valentine leukocidin (rPVL) on cytoskeleton rearrangement, and identified the signaling pathways involved in regulating the process in bovine mammary epithelial cells (BMECs) in vitro. Meanwhile, the underlying regulatory mechanism of baicalin for this process was investigated. The results showed that S. aureus induced cytoskeleton rearrangement in BMECs mainly through PVL. S. aureus and rPVL caused alterations in the cell morphology and layer integrity due to microfilament and microtubule rearrangement and focal contact inability. rPVL strongly induced the phosphorylation of cofilin at Ser3 mediating by the activation of the RhoA/ROCK/LIMK pathway, and resulted in the activation of loss of actin stress fibers, or the hyperphosphorylation of Tau at Ser396 inducing by the inhibition of the PI3K/AKT/GSK-3β pathways, and decreased the microtubule assembly. Baicalin significantly attenuated rPVL-stimulated cytoskeleton rearrangement in BMECs. Baicalin inhibited cofilin phosphorylation or Tau hyperphosphorylation via regulating the activation of RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β signaling pathways. These findings provide new insights into the pathogenesis and potential treatment in S. aureus causing bovine mastitis.

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