Crossreactivity of workshop monoclonal antibodies with canine blood leukocytes

Schuberth, Hans‐Joachim; Rabe, Hardis; Beer, A E; Leibold, W.

doi:10.1016/s0165-2427(97)00116-5

Cited by 4 publications

(2 citation statements)

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“…In some species a limited number of marker antibodies are available. Therefore, the search for monoclonal antibodies generated against antigens derived from other species for their cross-reactivity and systematic studies have been performed [ 18 , 20 , 25 – 28 ]. Some cross-reacting antibodies have been employed to study canine hematopoietic cells [ 29 , 30 ], canine lymphomas [ 31 ], canine lymphocyte subsets [ 32 ] or the canine leukocyte adhesion deficiency syndrome (CLAD) [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

“…Kwong et al [ 22 ] produced and tested two bovine monoclonal antibodies against ovine TNF-α and found one with satisfactory reactivity. Schuberth et al [ 18 ] tested cross-reactivity of 164 mAbs for pigs against canine leukocytes, and obtained 11 % of reactivity. Those data indicated that the recognition of conserved epitopes in evolutionarily distant species such as dogs and pigs was very low.…”

Section: Introductionmentioning

confidence: 99%

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Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining

et al. 2015

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BackgroundThe process for obtaining monoclonal antibodies against a specific antigen is very laborious, involves sophisticated technologies and it is not available in most research laboratories. Considering that most cytokines remain partially conserved among species during evolution, the search for antibody cross-reactivity is an important strategy for immunological studies in veterinary medicine. In this context, the amino acid sequence from human and canine cytokines have demonstrated 49–96 % homology, suggesting high probability of cross-reactivity amongst monoclonal antibodies. For this, 17 commercially available anti-human monoclonal antibodies [IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-10, IL-12, IL-13, IL-17A, IFN-γ (#1, #2), TNF-α (#1, #2) and TGF-β], were evaluated in vitro for intracellular cytokine detection in a stimulated canine blood culture by flow cytometry and confocal microscopy. Lymphocytes from peripheral blood of healthy and two unhealthy dogs were analyzed.ResultsEleven anti-human mAbs [IL-1α, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-12, IL-17A, TNF-α (#1, #2) and TGF-β] cross-reacted against canine intracellular cytokines. The specificity of the assays was not affected after Fc-blocking. Three anti-human cytokine mAbs [IL-4, IL-8 (#2) and TGF-β] when evaluated by confocal microscopy also cross-reacted with intracellular canine cytokines. The identification of human mAbs that cross-reacted with canine cytokines may support their use as immunological biomarkers in veterinary medicine studies.ConclusionThe identification of these 11 anti-human cytokine mAbs that cross-reacted with canine cytokines will be useful immunological biomarkers for pathological conditions by flow cytometry and fluorescence microscopy in dogs.

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