Crosslinking of caseins by human placental factor XIIIa

Protein cross-linking ability of sarcoplasmic proteins extracted from threadfin bream

“…k-Casein and b-casein were cross-linked by human placental factor XIIIa to a greater extent than was a-casein (Traoré & Meunier, 1991). In our study, protein substrates were incubated at 50 C for 10 min to induce the partial exposure of reactive glutaminyl and lysyl residues.…”

Incorporation of microbial transglutaminase into non-fat yogurt production

“…According to Cozzolino et al (2003), the different caseins showed similar reactivity with the microbial TGase, in contrast with the results obtained previously for other molecular forms of TGase. In fact, Ikura, Kometani, Yoshikawa, Sasaki, and Chiba (1980) reported a lower reactivity of k-casein compared with a S1 -and bcaseins with the guinea-pig liver enzyme, whereas a-casein was reported to act less effectively as an acyl donor substrate of the blood coagulation Factor XIII compared with both b-and k-caseins (Traore`& Meunier, 1991). The decrease in band intensities of caseins indicated that these milk protein fractions were good substrates for MTGase as reported in previous studies (O 'Sullivan, Kelly, & Fox, 2002;Dickinson, 1997;Han & Damodaran, 1996).…”

Identification of glutamine and lysine residues in Alzheimer amyloid βA4 peptide responsible for transglutaminase‐catalysed homopolymerization and cross‐linking to α2M receptor

“…Guinea pig liver transglutaminase and thermolysin were from Sigma, USA. Human placental factor XIII zymogen, was purified from Fibrogammin (Behringwerke, Germany, a gift from Dr. E. Schiiler) and activated by brief exposure to 0.5 M CaCl, according to [18]. [1,4-'4C]putrescine (109 mCi/mmol), [1,4-'Hlputrescine (20 Cilmmol) and "'1 (2.0 Ciipmol) were from Amersham, UK.…”