Crosslinking method of hyaluronic-based hydrogel for biomedical applications

Khunmanee, Sureerat; Jeong, Younghyen; Park, Hansoo

doi:10.1177/2041731417726464

Cited by 279 publications

(201 citation statements)

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“…No such effect was observed by Chen et al [18] when they used PEGDA during development of a HA hydrogel scaffold for ex vivo culture of limbal stem cells in a xenogeneic-free culture system. Crosslinkers are commonly used to overcome the poor solubility and physical properties of HA hydrogels, such as mechanical strength and gel formation time, but they may have a cytotoxic effect on some types of stem cells [27][28][29]. The biosafety and purity of HA hydrogels are critical factors in the design of materials for clinical application [28,30].…”

Section: Discussionmentioning

confidence: 99%

“…HA solubility and poor physical properties (e.g., mechanical strength, gel formation time and degradation in vivo) encourage the use of crosslinkers. However, some crosslinkers have been shown to be cytotoxic to stem cells [27][28][29]. Therefore, parameters such as the source and concentration of HA, nature of crosslinker, ratio of HA to crosslinker and buffer environment should be considered when designing HA hydrogel scaffolds for particular cell types.…”

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confidence: 99%

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A Hyaluronan Hydrogel Scaffold for Culture of Human Oral Mucosal Epithelial Cells in Limbal Stem-Cell Therapy

et al. 2019

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Hyaluronan (HA), a major component of the extracellular matrix, plays a key role in cell proliferation, growth, survival, polarization and differentiation. We investigated the optimization of a HA hydrogel scaffold for culture of human oral mucosal epithelial cells (OMECs) for potential application in limbal stem cell therapy. The effect of the optimized scaffold on OMEC cell sheet morphology, cell metabolic activity and expression of genes associated with stemness, adherence and cell damage was studied. The results indicate that HA hydrogels crosslinked with polyethylene glycol diacrylate (PEGDA) failed to support OMEC attachment and growth. However, HA hydrogel scaffolds dried for three days and coated with 1 mg/mL collagen IV produced a full OMEC sheet. Cell morphology was comparable to control after three weeks culture, maintaining 76% metabolic activity. Of apoptosis-related genes, the pro-apoptotic markers CASP3 and BAX2 were upregulated and downregulated, respectively, compared to control whereas the anti-apoptotic marker BCL2 was downregulated. The expression level of stemness genes ∆Np63α and ABCG2 was significantly higher than control. Genes associated with improved scar-less wound healing (integrin-αV) and protection of the ocular surface (cadherin-1) had~3-fold increased expression. These data suggest that our optimized HA-hydrogel scaffold could enhance culture of OMEC cell sheets for use in ocular reconstruction. Bioengineering 2019, 6, 97 2 of 17 (OMECs) from rabbits [4] and humans [5]. Later, other cell types such as embryonic stem cells [6], conjunctival epithelial cells [7], bone marrow-derived mesenchymal stem cells [8], epidermal adult stem cells [9], immature dental pulp stem cells [10], follicle bulge-derived stem cells [11] and umbilical cord-lining stem cells [12] were introduced. Cell sheets are typically cultured on a carrier scaffold for transplantation to the ocular surface [13].Choice of carrier scaffold is crucial to prevent differentiation and loss of stem-cell phenotype. The phenotypic state is an important indicator of healthy cells and it is known that the success rate of regeneration in damaged tissue depends on the degree of stemness in the transplant sheet rather than the number of cells [14]. Several natural and synthetic polymer materials have been developed for use as scaffolds such as human amniotic membrane, fibrin, siloxane hydrogel contact lenses, human anterior lens capsule, collagen, plastic compressed collagen, crosslinked collagen, electrospun and magnetically oriented scaffolds [15]. Several studies suggest hyaluronan (HA) hydrogel scaffolds as a potential carrier scaffold for use in treatment of LSCD. HA hydrogel scaffolds chemically cross-linked with a polyaspartamide derivative (PHEA-EDA) have been used as a substitute for amniotic membrane for delivery of human LECs [16]. Additionally, a 3D HA hydrogel with and without collagen type I has been shown to enhance the growth and differentiation of human corneal epithelial cells (hCECs) when co-cultured with adipose...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A Hyaluronan Hydrogel Scaffold for Culture of Human Oral Mucosal Epithelial Cells in Limbal Stem-Cell Therapy

et al. 2019

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“…Injectable scaffolds are superior to preformed scaffolds in terms of improved patient's compliance, ease of clinical implementation for the treatment of geometrically complex, and large lesions via minimally invasive techniques, such as arthroscopy [270]. This type of scaffolds can be used in minimally invasive surgical procedures; they completely fill the defect area and have good permeability, being hence promising biomaterials [271,272]. The technique can be effectively applied to deliver a wide range of bioactive agents, such as drugs, proteins, growth factors, and even living cells.…”

Section: Biomedical Applications Of Heteropolysaccharides-based Hybrimentioning

confidence: 99%

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Vasile¹,

Pamfil²,

Stoleru³

et al. 2020

Molecules

179

131

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New trends in biomedical applications of the hybrid polymeric hydrogels, obtained by combining natural polymers with synthetic ones, have been reviewed. Homopolysaccharides, heteropolysaccharides, as well as polypeptides, proteins and nucleic acids, are presented from the point of view of their ability to form hydrogels with synthetic polymers, the preparation procedures for polymeric organic hybrid hydrogels, general physico-chemical properties and main biomedical applications (i.e., tissue engineering, wound dressing, drug delivery, etc.).

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“…Chemically and physically crosslinked GAGs possess unique properties in terms of biodegradation and biocompatibility. These features were used to form cell scaffolds, drug delivery systems, and for wound healing (Khunmanee, Jeong, & Park, ). In addition, GAG‐based hydrogels offer interesting possibilities for bioprinting (Martini et al, ).…”

Section: Polysaccharidesmentioning

confidence: 99%

Carbohydrate‐based nanomaterials for biomedical applications

Gim

Zhu

Seeberger

et al. 2019

WIREs Nanomed Nanobiotechnol

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Carbohydrates are abundant biomolecules, with a strong tendency to form supramolecular networks. A host of carbohydrate-based nanomaterials have been exploited for biomedical applications. These structures are based on simple monoor disaccharides, as well as on complex, polymeric systems. Chemical modifications serve to tune the shapes and properties of these materials. In particular, carbohydrate-based nanoparticles and nanogels were used for drug delivery, imaging, and tissue engineering applications. Due to the reversible nature of the assembly, often based on a combination of hydrogen bonding and hydrophobic interactions, carbohydrate-based materials are valuable substrates for the creations of responsive systems. Herein, we review the current research on carbohydratebased nanomaterials, with a particular focus on carbohydrate assembly. We will discuss how these systems are formed and how their properties are tuned. Particular emphasis will be placed on the use of carbohydrates for biomedical applications.

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Crosslinking method of hyaluronic-based hydrogel for biomedical applications

Cited by 279 publications

References 103 publications

A Hyaluronan Hydrogel Scaffold for Culture of Human Oral Mucosal Epithelial Cells in Limbal Stem-Cell Therapy

A Hyaluronan Hydrogel Scaffold for Culture of Human Oral Mucosal Epithelial Cells in Limbal Stem-Cell Therapy

New Developments in Medical Applications of Hybrid Hydrogels Containing Natural Polymers

Carbohydrate‐based nanomaterials for biomedical applications

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