Crosslinked α-elastin biomaterials: towards a processable elastin mimetic scaffold

Leach, Jennie B.; Wolinsky, Jesse B.; Stone, Phillip J.; Wong, Joyce Y.

doi:10.1016/j.actbio.2004.12.001

Cited by 117 publications

(66 citation statements)

References 42 publications

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“…The elastic modulus could to some extent be modulated by the degree of crosslinking. In a preliminary experiment, vascular smooth muscle cells adhered to elastin discs, but showed decreased proliferation on the material [147]. We recently showed that crosslinked scaffolds composed of insoluble collagen and solubilised elastin not only induced angiogenesis, but also increased elastic fi bre synthesis, as shown with antibodies against rat fi brillin-1 and rat elastin.…”

Section: Figure 5 Transmission Electron Micrographs (A C) and Scannmentioning

confidence: 93%

Elastin as a biomaterial for tissue engineering

et al. 2007

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Section: Figure 5 Transmission Electron Micrographs (A C) and Scannmentioning

confidence: 93%

Elastin as a biomaterial for tissue engineering

et al. 2007

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“…Elastin can also be cross-linked by chemical (e.g. using aldehydes and epoxy groups) [202,203], enzymatic [204] and physical processes [205]. These strategies allow an efficient binding to amino acids side chains, low antigenicity and improved mechanical strength [201].…”

Section: Cellulose and Its Derivativesmentioning

confidence: 99%

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

et al. 2013

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a b s t r a c tDiabetic foot ulcers (DFUs) are a chronic, non-healing complication of diabetes that lead to high hospital costs and, in extreme cases, to amputation. Diabetic neuropathy, peripheral vascular disease, abnormal cellular and cytokine/chemokine activity are among the main factors that hinder diabetic wound repair. DFUs represent a current and important challenge in the development of novel and efficient wound dressings. In general, an ideal wound dressing should provide a moist wound environment, offer protection from secondary infections, remove wound exudate and promote tissue regeneration. However, no existing dressing fulfills all the requirements associated with DFU treatment and the choice of the correct dressing depends on the wound type and stage, injury extension, patient condition and the tissues involved. Currently, there are different types of commercially available wound dressings that can be used for DFU treatment which differ on their application modes, materials, shape and on the methods employed for production. Dressing materials can include natural, modified and synthetic polymers, as well as their mixtures or combinations, processed in the form of films, foams, hydrocolloids and hydrogels. Moreover, wound dressings may be employed as medicated systems, through the delivery of healing enhancers and therapeutic substances (drugs, growth factors, peptides, stem cells and/or other bioactive substances). This work reviews the state of the art and the most recent advances in the development of wound dressings for DFU treatment. Special emphasis is given to systems employing new polymeric biomaterials, and to the latest and innovative therapeutic strategies and delivery approaches.

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“…Bulk elastic modulus "K" was calculated by applying the relation between "G", "K", and the Poisson coefficient (υ) 32 . Poisson coefficient was estimated to be 0.49 33 (Supplementary Information).…”

Section: Macroscopic Properties: Rheologymentioning

confidence: 99%

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

et al. 2014

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ABSTRACT. Although significant progress has been made in the area of injectable hydrogels for biomedical applications and model cell niches, further improvements are still needed, especially in terms of mechanical performance, stability and biomimicry of the native fibrillar architecture found in the extracellular matrix (ECM). This work focuses on the design and production of a silk-elastin-based injectable multiblock co-recombinamer that spontaneously forms a stable physical nanofibrillar hydrogel under physiological conditions. That differs from previously reported silk-elastin-like polymers on a major content and predominance of the elastin-like part, as well as a more complex structure and behavior of such part of the molecule, which is aimed to obtain well defined hydrogels.Rheological and DSC experiments showed that this system displays a coordinated and concomitant dual gelation mechanism. In a first stage, a rapid, thermally driven gelation of the co-recombinamer solution takes place once the system reaches body temperature due to the thermal responsiveness of the elastinlike (EL) parts and the amphiphilic multiblock design of the co-recombinamer. A bridged micellar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 structure is the dominant microscopic feature of this stage, as demonstrated by AFM and TEM.Completion of the initial stage triggers the second, which comprises a stabilization, reinforcement, and microstructuring of the gel. FTIR analysis shows that these events involve the formation of β-sheets around the silk motifs. The emergence of such β-sheet structures leads to the spontaneous selforganization of the gel into the final fibrous structure. Despite the absence of biological cues, here we set the basis of the minimal structure that is able to display such a set of physical properties and undergo microscopic transformation from a solution to a fibrous hydrogel. The results point to the potential of this system as a basis for the development of injectable fibrillar biomaterial platforms towards a fully functional, biomimetic, artificial extracellular matrix and cell niches.

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Crosslinked α-elastin biomaterials: towards a processable elastin mimetic scaffold

Cited by 117 publications

References 42 publications

Elastin as a biomaterial for tissue engineering

Elastin as a biomaterial for tissue engineering

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

Self-Organized ECM-Mimetic Model Based on an Amphiphilic Multiblock Silk-Elastin-Like Corecombinamer with a Concomitant Dual Physical Gelation Process

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