Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern

Segura, Tatiana; Anderson, Brian C.; Chung, Peter; Webber, Rebecca; Shull, Kenneth R.; Shea, Lonnie D.

doi:10.1016/j.biomaterials.2004.02.067

Cited by 340 publications

(236 citation statements)

References 53 publications

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“…HA is a natural component of many connective tissues, has a significant role in wound healing, and has been used in regenerative medicine [20,21]. The hydrogels described herein were more than 94% water, similar to previous reports [22]. Enzymatic degradation of HA was initiated by HAase, a cell secreted enzyme [30], which allows hydrogel degradation in response to cell growth.…”

Section: Discussionsupporting

confidence: 83%

“…HAAcryl was obtained by a two-step process that involves the initial modification of hyaluronic acid with adipic acid dihydrazide (HA-AAD) as previously described [22], which was subsequently modified with NHS-PEG-Acryl. HA-AAD was characterized using a trinitrobenzene sulfonic acid (TNBS) assay (Pierce, Rockford, IL) to determine the percent of carboxyl groups replaced with hydrazide groups.…”

Section: Synthesis and Characterization Of Acrylated Hyaluronic Acidmentioning

confidence: 99%

“…Bovine testicular hyaluronidase was used for HA degradation studies, with concentration chosen based on previous reports [22,27]. For plasmid release, the solution above the hydrogel was sampled (200 μl) with replacement.…”

Section: Hydrogel Degradation and Dna Releasementioning

confidence: 99%

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Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Wieland

Houchin-Ray

Shea

2007

Journal of Controlled Release

121

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Hydrogels have been widely used in tissue engineering as a support for tissue formation or to deliver non-viral gene therapy vectors locally. Hydrogels that combine these functionalities can provide a fundamental tool to promote specific cellular processes leading to tissue formation. This report investigates controlled release of gene therapy vectors from hydrogels as a function of the physical properties for both the hydrogel and the vector. Hydrogels were formed by photocrosslinking acrylmodified hyaluronic acid (HA) with a 4-arm poly(ethylene glycol) (PEG) acryl. The polymer content, and relative composition of HA and PEG modulated the swelling ratio, water content, and degradation, which can influence transport of the vector through the hydrogel. All hydrogels had a water content of 94% or higher, though the water content and swelling ratio increased with a decrease in the PEG:HA ratio. Plasmids were stably incorporated into the hydrogel, with a majority of the release occurring during the initial 2 days. For incubation in buffer, the cumulative release increased with a decreasing PEG or increasing HA content, with approximately 20% to 80% released during the first week depending on the hydrogel composition. Hydrogels incubated in hyaluronidase, an enzyme that degrades HA, significantly increased plasmid release for hydrogels containing 4% PEG and 4% HA-Acryl. The encapsulation of plasmid complexed with polyethylenimine had less than 14% of the complexes released from the hydrogel both in the presence and absence of hyaluronidase. The limited release of the complexes likely results from the complex size and interactions between the vector and hydrogel. These studies demonstrate the dependence of non-viral vector release on the physical properties of the hydrogel and the vector, suggesting vector and hydrogel designs for maximizing localized delivery of non-viral vectors.

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Section: Discussionsupporting

confidence: 83%

Section: Synthesis and Characterization Of Acrylated Hyaluronic Acidmentioning

confidence: 99%

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Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Wieland

Houchin-Ray

Shea

2007

Journal of Controlled Release

121

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“…Sulfo-NHS was dissolved in the same solution at the final constant concentration of 11 mg/mL. The PEI-(HA/HA + ) 9 -HA and PEI-(HA/HA + ) 10 coated glass slides were introduced in 24-well culture plates and put in contact with 0.5 mL of the EDC/S mixture during 12 hours at 4°C. Rinsing was performed three times with a 0.01 M NaCl solution for one hour at pH 8 and then the films were rinsed with a 0.15 M NaCl solution at pH 7.4 before being placed in contact with the culture medium.…”

Section: Film Characterization and Cross-linking By Ftirmentioning

confidence: 99%

“…Hyaluronan has not only peculiar physical properties in terms of hydration, viscosity and hydrogel formation but it becomes more and more investigated for its specific biological and bioactive properties and its implications in cell adhesion properties 5,6 and motility 7 . One of the main applications of hyaluronan concerns tissue engineering, where it has been mostly used to form a wide variety of hydrogels [8][9][10] . Several strategies have been developed to prepare these gels and rely usually on the introduction of chemically modified groups or on its complexation with cationic biopolymers (such as chitosan or collagen at acidic pHs) such as to form composite hydrogels 11,12 .…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer Films from Hyaluronan and Amine-Modified Hyaluronan

et al. 2007

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Hyaluronan is a polysaccharide that is increasingly investigated for its role in cellular adhesion and for the preparation of biomimetic matrices for tissue engineering. Hyaluronan gels are prepared for application as space fillers whereas hyaluronan films are usually obtained by adsorbing or grafting a single hyaluronan layer onto a biomaterial surface. Here, we examine the possibility to employ the layer-by-layer technique to deposit thin films of cationic modified hyaluronan (HA + ) and hyaluronan (HA) of controlled thicknesses. The buildup conditions are investigated and growth is compared to that of other polyelectrolyte multilayer films containing either HA as polyanion or HA + as polycation. The films could be formed in a low ionic strength medium but required to be cross-linked prior to be put in contact with physiological medium. NIH3T3 fibroblasts were perfectly viable on selfassembled hyaluronan films with however a preference for hyaluronan ending films. These findings point out the possibility to tune the thickness of thin hyaluronan films at the nanometer scale. Such architectures could be employed for investigating cell/substrate interactions or for functionalizing biomaterial surfaces.

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Hydrogels

Ray

2022

Kirk-Othmer Encyclopedia of Chemical Technology

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Hydrogels are prepared by physical or chemical cross‐linking of water‐soluble polymers. Natural, semisynthetic, and synthetic polymers may be cross‐linked physically or by a cross‐linker to produce the network structure of a hydrogel. Because of cross‐linking these polymers are not soluble in water. However, hydrogels can absorb huge amount of water, resulting in significant swelling of their three‐dimensional network structure. The swelling of a responsive and smart hydrogel strongly depends on external stimuli such as pH, temperature, salt concentration, light, and magnetic and electric fields. Hydrogels are widely reported for various applications. In fact, in recent years the research on hydrogel has been found to increase at an exponential rate, with around 8000 publications in 2021. In this article, the origin, history, theoretical modeling, synthesis, properties, classifications, characterization, and new applications of hydrogels have been discussed in detail with the citation of references till 2021.

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Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern

Cited by 340 publications

References 53 publications

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Non-viral vector delivery from PEG-hyaluronic acid hydrogels

Layer-by-Layer Films from Hyaluronan and Amine-Modified Hyaluronan

Hydrogels

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