Crosslinked collagen/chitosan matrix for artificial livers

Wang, X.H.; Li, D.P.; Wang, W.J.; Feng, Qingling; Cui, Fuzhai; Xu, Yingxin; Xu, Song; Werf, Mark van der

doi:10.1016/s0142-9612(03)00170-4

Cited by 289 publications

(176 citation statements)

References 27 publications

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“…The addition of collagen to a ceramic structure can provide many additional advantages to surgical applications: shape-control, spatial adaptation, increased particle and defect walladhesion, and the capability to favour clot-formation and stabilisation (Scabbia and Trombelli, 2004). cross-linked collagen/chitosan (Kim et al, 2001;Ma et al, 2003;Wang et al, 2003;Chalonglarp et al, 2006) as well as gelatine/chitosan (Kim et al, 2005;Chiono et al, 2008) matrices were presented as a promising biomaterial for tissue engineering, to be used in several specific areas, such as drug delivery, wound dressings, sutures, nerve conduit, and matrix templates for tissue engineering. Human connective tissues do not contain chitosan, but it has structural similarity to glucosaminoglycan (GAG), mostly components of ECM.…”

Section: Combination With Other Biopolymersmentioning

confidence: 99%

“…Chitosan, because of it cationic nature, can promote cell adhesion, can act as modulator of cell morphology, differentiation, movements, synthesis and function. It is reported that chitosan induces fibroblasts to release interleukin-8, which is involved in migration and proliferation of fibroblasts and vascular endothelial cells, but, also promotes surface-induced thrombosis and embolization, which limits its application in blood-containing biomaterials (Wang et al, 2003). Chitosan addition enhances poor mechanical properties of gelatine and influence on more controllable biodegradation rate.…”

Section: Combination With Other Biopolymersmentioning

confidence: 99%

See 1 more Smart Citation

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

223

250

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Section: Combination With Other Biopolymersmentioning

confidence: 99%

Section: Combination With Other Biopolymersmentioning

confidence: 99%

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

223

250

View full text Add to dashboard Cite

“…The mechanical properties and biodegradation rates of the collagen and chitosan are not good, especially in aqueous media (Wang et al 2003). Collagen molecules are usually cross-linked by chemi- cal agents such as glutaraldehyde (GE) and formaldehyde.…”

Section: Discussionmentioning

confidence: 99%

A cost-effective and simple culture method for primary hepatocytes

Aday

Hasırcı

Gürhan

2011

Animal Cells and Systems

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Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

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“…For C. crispus biomass, the main bands relate to -OH overlapping (3283 cm −1 ) [26], C-H stretching (2924 cm −1 ) [27], C=C stretching (1644 cm −1 ) [34], N-H bending and C-N stretching (1531 cm −1 ) [35], COO− symmetric stretching (1416 cm −1 ) [29,30], S-O stretching (1152 and 1221 cm −1 ) [36,37], C−O−C antisym. stretching (1152 and 1034 cm −1 ) [32,33], and CH bending (696 cm −1 ) [38]. In a word, the main functional groups on L. digitata are hydroxyl and carboxyl groups, while for C. crispus, besides these two reactive groups, sulfate groups are also identified.…”

Section: Characterizationmentioning

confidence: 97%

“…The band at 2935 cm −1 is assigned to the C-H stretching [27]. The peaks at 1605, 1416, 1250, and 1022 cm −1 represent COOasymmetric stretching [28], COO-symmetric stretching [29,30], CH3 symmetric bending [31], and C-O-C anti-symmetric stretching [32,33], respectively. For C. crispus biomass, the main bands relate to -OH overlapping (3283 cm −1 ) [26], C-H stretching (2924 cm −1 ) [27], C=C stretching (1644 cm −1 ) [34], N-H bending and C-N stretching (1531 cm −1 ) [35], COO− symmetric stretching (1416 cm −1 ) [29,30], S-O stretching (1152 and 1221 cm −1 ) [36,37], C−O−C antisym.…”

Section: Characterizationmentioning

confidence: 99%

A Comparison of Palladium Sorption Using Polyethylenimine Impregnated Alginate-Based and Carrageenan-Based Algal Beads

2018

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Two kinds of algal beads were prepared using a carrageenan-based alga (Chrondrus crispus) and an alginate-based alga (Laminara digitata) ionotropically gelled with K(I) and Ca(II), respectively: the process consists of biopolymer partial extraction followed by hydrogel formation. The beads were modified with branched polyethylenimine (bPEI) and glutaraldehyde (GA) using the impregnation method to improve their sorption capacity for Pd(II) in acid solution. SEM-EDX and FTIR techniques were used for characterizing the beads. The impacts of pH and presence of anions, cations, and Pt(IV) were studied in batch experiments. The beads were also applied for Pd(II) recovery from synthesized leaching liquors of a spent catalyst and a car catalytic converter via the sorption-desorption process. Results show that Pd is concentrated in the outer layer of L. digitata-bPEI-GA composite (LD/PEI) beads, while in the case of the C. crispus-bPEI-GA composite (CC/PEI), it is homogenously distributed in the whole mass of the sorbents. The difference is attributed to the repulsive force of the outer Ca(II)-alginate barrier of LD/PEI beads that makes it difficult for the branched polymer PEI to penetrate through the layer and be immobilized in the inner compartment. As a result, LD/PEI beads possess a lower maximum sorption capacity, but a slightly faster uptake at pH 1 than CC/PEI beads. In addition, CC/PEI beads present a better recovery performance compared to LD/PEI beads when applied for the treatment of synthesized leaching liquors.

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Crosslinked collagen/chitosan matrix for artificial livers

Cited by 289 publications

References 27 publications

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

A cost-effective and simple culture method for primary hepatocytes

A Comparison of Palladium Sorption Using Polyethylenimine Impregnated Alginate-Based and Carrageenan-Based Algal Beads

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