Crossing experiments and behavioral observations reveal reproductive incompatibility among three putative species of the whitefly <i>Bemisia tabaci</i>

Wang, Peng; Ruan, Yongming; Liu, Shu‐Sheng

doi:10.1111/j.1744-7917.2010.01353.x

Cited by 40 publications

(37 citation statements)

References 38 publications

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“…The two most distinct populations were BJabo, which might be because of geographic distance, and CPsoj, which might be because of the greater adaptation of the pest to the host plant and/or to the reproductive isolation. Previous studies have confirmed that specific populations reproductively isolated because of copulation and post-copulation barriers (Wang et al, 2010;Xu et al, 2010;Sun et al, 2011).…”

Section: Genetic Structurementioning

confidence: 75%

Population variability of Bemisia tabaci (Genn.) in different hosts

Valle

Lourenção²,

Zucchi³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The silverleaf whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae), is a cryptic species complex that contains some of the most damaging pests in tropical and subtropical regions. Recent studies have indicated that this complex is composed of at least 24 distinct and morphologically indistinguishable species that mainly differ in their ability to transmit phytoviruses, adapt to hosts, and induce physiological changes in certain hosts. The importance of this species has been increasing worldwide, because it serves as a phytovirus vector, particularly for geminiviruses, in economically important crops. Here, we aimed to examine the population variability (2013) G.E. do Valle et al.-soybean], UBman [Ubatuba (SP, Brazil) -cassava], and PEmel [Petrolina (PE, Brazil) -melon]. Thirteen polymorphic loci with 50 alleles were observed, with an average of 2.37 (range: 2.00-2.91) alleles per population. The UBman and PEmel B. tabaci populations were the most differentiated, which was probably caused by insect adaptation to the host plant and the use of insecticides. A 33.87% interpopulation variation was observed, indicating that microsatellites may be used to measure differentiation among these B. tabaci populations. Based on the comparison of microsatellites in the current study, only the Middle East-Asia Minor 1 population of B. tabaci was found in the six populations.

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Section: Genetic Structurementioning

confidence: 75%

Population variability of Bemisia tabaci (Genn.) in different hosts

Valle

Lourenção²,

Zucchi³

et al. 2013

Genet. Mol. Res.

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“…For six putative species, all potential crosses would include six intra‐species control treatments and 30 inter‐species crosses. Previously, 12 inter‐species crosses were conducted between Middle East‐Asia Minor 1, Mediterranean, Asia II 1 and Asia II 3 (Zang & Liu, 2007; Xu et al ., 2010; Wang et al ., 2010b; Sun et al ., 2011). However, the two reciprocal crosses between Middle East‐Asia Minor 1 and Asia II 3 were conducted using single‐pair mating only but not group‐mating (Zang & Liu, 2007), thus these two reciprocal crosses were repeated here using group mating.…”

Section: Methodsmentioning

confidence: 99%

The presence of six cryptic species of the whitefly Bemisia tabaci complex in China as revealed by crossing experiments

et al. 2010

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Recent phylogenetic analysis using mitochondrial cytochrome oxidase I (mtCOI) sequences of Bemisia tabaci worldwide indicates that the whitefly comprises at least 24 morphologically indistinguishable but genetically distinct cryptic species. While evidence of reproductive isolation has been reported for some of the putative species, more extensive crossing experiments are required to clarify the systematics of this species complex. In this study, we established laboratory cultures for six putative species of B. tabaci collected in China. We conducted 22 inter‐species crosses among the six putative species. The data and those reported previously were collated, and the combined dataset covered all the 30 possible inter‐species crosses among the six putative species. Intra‐species controls always produced female and male progeny and the proportions of females in the first generation (F1) ranged from 56% to 70%. However, in inter‐species crosses female progeny were rarely produced, and the few F1 females produced in four of the 30 inter‐species crosses were either sterile or significantly weaker in viability. These results demonstrate a pattern of complete reproductive isolation among the six putative species and show that they are six cryptic species in the B. tabaci complex.

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“…The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a species complex that contains some of the most destructive pests of fiber, vegetable, and ornamental crops [1], [2], [3], [4] and causes severe economic losses every year by direct feeding, excreting honeydew and transmitting plant viruses [5], [6], [7], [8]. The whitefly is known to colonize over 600 plant species with high population growth rate and remarkable adaptability to environmental stresses [2], [9], making it rather difficult to manage B. tabaci and the virus it transmits.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

Xia¹,

Zhang²,

Zhang³

et al. 2013

PLoS ONE

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BackgroundThe fungal pathogen, Beauveria bassiana, is an efficient biocontrol agent against a variety of agricultural pests. A thorough understanding of the basic principles of insect-fungus interactions may enable the genetic modification of Beauveria bassiana to enhance its virulence. However, the molecular mechanism of insect response to Beauveria bassiana infection is poorly understood, let alone the identification of fungal virulent factors involved in pathogenesis.Methodology/Principal FindingsHere, next generation sequencing technology was applied to examine the expression of whitefly (Bemisia tabaci) genes in response to the infection of Beauveria bassiana. Results showed that, compared to control, 654 and 1,681genes were differentially expressed at 48 hours and 72 hours post-infected whiteflies, respectively. Functional and enrichment analyses indicated that the DNA damage stimulus response and drug metabolism were important anti-fungi strategies of the whitefly. Mitogen-activated protein kinase (MAPK) pathway was also likely involved in the whitefly defense responses. Furthermore, the notable suppression of general metabolism and ion transport genes observed in 72 hours post-infected B. tabaci might be manipulated by fungal secreted effectors. By mapping the sequencing tags to B. bassiana genome, we also identified a number of differentially expressed fungal genes between the early and late infection stages. These genes are generally associated with fungal cell wall synthesis and energy metabolism. The expression of fungal cell wall protein genes might play an important role in fungal pathogenesis and the dramatically up-regulated enzymes of carbon metabolism indicate the increasing usage of energy during the fungal infection.Conclusions/SignificanceTo our knowledge, this is the first report on the molecular mechanism of fungus-whitefly interactions. Our results provide a road map for future investigations on insect-pathogen interactions and genetically modifying the fungus to enhance its efficiency in whitefly control.

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Crossing experiments and behavioral observations reveal reproductive incompatibility among three putative species of the whitefly Bemisia tabaci

Cited by 40 publications

References 38 publications

Population variability of Bemisia tabaci (Genn.) in different hosts

Population variability of Bemisia tabaci (Genn.) in different hosts

The presence of six cryptic species of the whitefly Bemisia tabaci complex in China as revealed by crossing experiments

Analysis of Whitefly Transcriptional Responses to Beauveria bassiana Infection Reveals New Insights into Insect-Fungus Interactions

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