Crossbreeding of Phyllostachys species (Poaceae) and identification of their hybrids using ISSR markers

Lin, Xiao; Lou, Yong Feng; Liu, J.; Peng, Jie; Liao, Gwo-Ing; Wei, Fang

doi:10.4238/vol9-3gmr855

Cited by 32 publications

(21 citation statements)

References 16 publications

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“…Similarly, Carvalho et al (2005) have used 30 ISSR primers to confirm the interspecific hybrids of 3 crosses of the Triticeae tribe and found that 13-20 ISSR primers gave polymorphic DNA bands between male and female parents in each cross and could verify their interspecific hybrids. Eight ISSR primers have also been used successfully in the identification of interspecific bamboo hybrids (Lin et al, 2010). Moreover, the use of ISSR to distinguish hybrids has been reported in artichoke, clover, and mandarin (Scarano et al, 2002;Dabkevičienė et al, 2008;Bianco et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The F 1 hybrids are usually identified based on their morphological characteristics, a process that is successful if at least one of these characteristics is intermediate between the male and female parents or highly similar to the respective male parents. However, identification based on morphological characteristics can be difficult (especially at early stages), ambiguous, time-consuming, and dependent on environment (Asif et al, 2006;Lin et al, 2010). In addition, the parents in some crosses might have highly similar morphological characteristics or produce F 1 hybrids with morphological characteristics that cannot be visually distinguished from their respective female parents and hence progeny arising from self-pollination.…”

Section: Introductionmentioning

confidence: 99%

“…Because of its simplicity, efficiency, high reproducibility, and non-reliance on prior genomic sequence information, it has been widely used in applications including genetic diversity, genetic relationship, and germplasm analyses, seed purity evaluations, gene/quantitative trait loci mapping, marker-assisted selection, and evolutionary study in various plants including mungbean (Ajibade et al, 2000;Reddy et al, 2002;Wong et al, 2005;Somta and Srinives, 2007;Abbas et al, 2010;Tantasawat et al, 2010a,b;Vir et al, 2010;Li et al, 2011;Mudibu et al, 2011). Its application in the identification and confirmation of hybrids and parentage has also been reported in artichoke, bamboo, clover, mandarin, Penstemon, and the Triticeae tribe (Wolfe et al, 1998;Scarano et al, 2002;Carvalho et al, 2005;Dabkevičienė et al, 2008;Lin et al, 2010;Bianco et al, 2011). In this study, we used ISSR markers to verify 6 putative F 1 hybrids with morphological characteristics that could not be visually discriminated from the self-pollinated progeny of their female parents.…”

Section: Introductionmentioning

confidence: 99%

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Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

Khajudparn

Prajongjai²,

Poolsawat³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Mungbean improvement via hybridization requires the identification of true F 1 hybrids from controlled crosses before further generations of selfing/crossing and selection. We utilized inter-simple sequence repeat (ISSR) markers for identifying putative F 1 hybrids from six cross combinations whose morphological characteristics were very similar to those of their respective female parents and could not be visually discriminated from the self-pollinated progeny. Based on 10 ISSR primers, polymorphisms were found between female and male parents of all six cross combinations. The highest value of genetic differentiation (21.4%) was found between male and female parents of the SUT3 x M5-1 cross. These 10 ISSR primers gave 2.8-25.0% polymorphism between male and female parents, with a mean of 12.1%, and 0-13.0% polymorphism between F 1 hybrid and female parents, with a mean of 4.8%. F 1 hybrids of all six cross combinations could be differentiated from the self-pollinated progeny of their female parents by using only either ISSR 841 or 857 primers, together with the ISSR 835 primer. We conclude that ISSR markers are useful and efficient for identifying mungbean F 1 hybrids in controlled crosses

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

Khajudparn

Prajongjai²,

Poolsawat³

et al. 2012

Genet. Mol. Res.

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“…Both methodologies amplify products from multiple loci and have been successfully used for genotyping , Bornet and Branchard 2001, Lin et al 2010, genetic linkage maps , Arcade et al 2000, genetic diversity (Davila et al 2007, Okun et al 2008) and somaclonal variation (Leroy et al 2000), on different species such as European and Japanese larch, Eucalyptus grandis, Phyllostachys and Agave species, among others.…”

Section: Introductionmentioning

confidence: 99%

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Moraga-Suazo¹,

Hasbún²,

Balocchi³

et al. 2012

Bosque (Valdivia)

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SUMMARYPinus radiata is a conifer of recognized economic value. Studies at genetic and molecular level to support breeding programs of radiata pine have been conducted with difficulty due to its large and complex genome. The development of molecular markers has allowed performing studies on genetic variation, identification of clones and construction of linkage maps. In order to increase the genetic molecular markers in P. radiata, two inter simple sequence repeat (ISSR) markers and twelve selective amplifications of microsatellite polymorphic loci (SAMPL) primer combinations were tested, using a first-generation full-sib family of 86 individuals. Polymerase chain reaction (PCR) products were visualized by capillary electrophoresis and polymorphism was detected by presence or absence of a particular fragment in one P. radiata parental. A total of 18 polymorphic fragments were found for two ISSR primers tested, with an average segregation distortion of 33 %. SAMPL markers yielded 85 polymorphic fragments with eight primer combinations, with an average of 22.3 % segregation distortion. Both methodologies showed a good reproducibility, with easy implementation and useful for genetic analysis on P. radiata.Key words: capillary electrophoresis, segregation distortion, forest trees. RESUMENPinus radiata es una conífera de reconocido valor económico, en la cual los estudios a nivel genético y molecular, han sido conducidos con dificultad en programas de mejoramiento debido a su genoma complejo y de gran tamaño. El desarrollo de marcadores moleculares ha permitido realizar estudios de variación genética, validar clones y construir mapas de ligamiento. Para lograr incrementar la disponibilidad de marcadores moleculares en P. radiata, se examinaron dos marcadores ISSR (inter simple sequence repeat) y 12 combinaciones de partidores SAMPL (selective amplification of microsatellite polymorphic loci), usando una familia F1 de 86 hermanos completos de primera generación. Los productos de PCR (polymerase chain reaction) se visualizaron mediante electroforesis capilar y la selección de fragmentos polimórficos se efectuó a base de la presencia o ausencia de un fragmento en uno de los parentales de la familia de P. radiata. Un total de 18 fragmentos polimórficos fueron encontrados para los dos partidores ISSR, con una distorsión de segregación promedio de 33 %. Los marcadores SAMPL generaron 85 fragmentos polimórficos empleando ocho combinaciones, con un promedio de 22,3 % de distorsión de segregación. Ambas metodologías mostraron buena reproducibilidad, fácil implementación y utilidad para análisis genético en P. radiata.Palabras clave: electroforesis capilar, distorsión de segregación, especies forestales.

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“…Because bamboos flowering is unpredictable and has a long interval, the manipulation of bamboo crossbreeding is difficult (Lin et al, 2010a(Lin et al, , 2010bLu et al, 2009). Gene transformation is another efficient approach to increase productivity and quality in plants.…”

Section: Introductionmentioning

confidence: 99%

Bamboo Regeneration via Embryogenesis and Organogenesis

Lin¹,

Huang²,

Wei³

2012

Embryogenesis

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Crossbreeding of Phyllostachys species (Poaceae) and identification of their hybrids using ISSR markers

Cited by 32 publications

References 16 publications

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

Establishment and optimization of ISSR and SAMPL molecular markers as a tool for breeding programs of Pinus radiata

Bamboo Regeneration via Embryogenesis and Organogenesis

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