Crossbreeding East African Highland Bananas: Lessons Learnt Relevant to the Botany of the Crop After 21 Years of Genetic Enhancement

Batte, Michael; Swennen, Rony; Uwimana, Brigitte; Akech, Violet; Brown, Allan; Tumuhimbise, Robooni; Hovmalm, Helena Persson; Geleta, Mulatu; Ortíz, Rodomiro

doi:10.3389/fpls.2019.00081

Cited by 55 publications

(65 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some self-pollinations of Musa acuminata clones yielded less seed than crosses implying that there are some levels of pollen compatibility on stigmas in banana. Batte, et al (2019) Though maximum temperature and average temperature measurement accounted for the most variability in data, they may not be entirely responsible for seed set in Musa. Nonetheless, they were consistently ranked highest by the rst PC in EACB pollinated with or without PGM.…”

Section: Discussionmentioning

confidence: 92%

“…It is also not clear when weather most affects seed set and what attribute accounts for most of the variability. Batte et al (2019) reported that the month of pollination had a non-signi cant effect on pollination success but seed set differed between years in EAHB. Moreover, variability between years is smaller than the variability between seasons or months (Ssebuliba et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…However, at 60 DBP, 8 of 12 considered weather attributes became associated with seed set in 'Mshale' and 9 of 12 for 'Nshonowa,' 'Enzirabahima' still had none.These differences may portray same weather conditions having different effects on fertility because of differences in developmental stages even if owering is at the same time. The month pollination effects could have therefore been confounded within pooled cultivars inBatte, et al (2019)…”

mentioning

confidence: 99%

See 2 more Smart Citations

Seed Set Patterns in East African Cooking Bananas (Musa spp.) are Dependent on Weather Before, During, and After Pollination

Waniale

Mukasa

Tugume

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Abstract Background: Seed set in banana (Musa spp.) is influenced by weather but the most critical weather attribute(s) and the critical period are unknown. Such information is of paramount importance to increase seed set for banana breeding programs. Three female fertile East African cooking bananas (EACBs), ‘Enzirabahima’ (AAA), ‘Mshale’ (AA), and ‘Nshonowa’ (AA) were pollinated with the highly male fertile wild banana ‘Calcutta 4’ (AA). At full maturity, bunches were harvested and ripened and seeds extracted from ripe fruit pulp. Seed set was then correlated with weather before, during, and after pollination.Results: Seed set was positively correlated with high temperatures (r=0.172 – 0.488), solar radiation (r=0.181 – 0.282) and negatively correlated with rainfall (r=-0.214 – -0.238) and relative humidity (RH) (r=-0.158 – -0.438) between 75 and 15 days before pollination (DBP). The pattern of weather association was cultivar-dependent with ‘Nshonowa’ having the strongest significant associations. At the time of pollination, high average temperatures were critical for seed set in ‘Enzirabahima’ (r=0.214, P<0.01) while high morning RH was critical for ‘Mshale’ (r=0.299, P<0.01). After pollination, high morning temperatures were associated with seed set (r=0.150 – 0.429) between 15 days to 90 days after pollination (DAP). High average temperatures were negatively correlated with seed set in ‘Mshale’ and ‘Nshonowa’ from 45 DAP to time of harvest (r=-0.208 – -0.344). Coefficients of correlation were generally highest 15 DBP especially for ‘Mshale’ and ‘Nshonowa.’ Principle component analysis showed that average and maximum temperature are the most important variables in the entire data set. Conclusion: Coefficients of correlation were generally less than 0.5 partly as a result of weather involvement in seed set at several floral development stages; before, during, and after pollination. The most critical developmental stage is 15 DBP especially for ‘Mshale’ and ‘Nshonowa’ as they had the high correlation coefficients. Average temperature should be the main focus for seed set increase in banana.

show abstract

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Seed Set Patterns in East African Cooking Bananas (Musa spp.) are Dependent on Weather Before, During, and After Pollination

Waniale

Mukasa

Tugume

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…This suggests that there are more factors that are yet to be identified and considered. Batte et al (2019) also found no month effects for pollination success of EAHBs after 21 years of crossbreeding. This raises questions about the underlying causes of observations made and how understanding these factors can ultimately lead to overcoming the fertility crisis in Musa.…”

Section: Introductionmentioning

confidence: 83%

Seed Set Patterns in East African Cooking Bananas are Asymmetric in Bunches and Fruits

Waniale¹,

Mukasa²,

Tugume³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Low female fertility in bananas is the biggest hurdle for banana breeding. The aim of this study was to determine seed set patterns in East African cooking bananas EACBs to inform future decisions on a more targeted approach of increasing seed set and subsequently banana breeding efficiency. Matooke (AAA) and Mchare (AA) bananas are genetically distinct but belong to the same genetic complex, they referred to as EACBs. Seed set patterns in ‘Enzirabahima’ (AAA), ‘Mshale’ (AA) and ‘Nshonowa’ (AA) all with residual fertility were examined after hand pollination with a highly male fertile wild banana ‘Calcutta 4’ (AA). Results: Seed set in ‘Enzirabahima’ is predominant in distal hands. Mchare cultivars have a slightly more even distribution of seeds in their hands compared to ‘Enzirabahima.’ There is a gradual increase in seed set from proximal to distal hands with a slight drop in the last hand. This pattern is more definite in ‘Enzirabahima’ and ‘Mshale’ while ‘Nshonowa’ has a somewhat inconsistent pattern. There is also a drop in seed set per 100 fruits per hand from small to larger bunches. However, larger bunches have a higher pollination success compared to smaller bunches. They therefor set more seed on 100 fruits per hand and per bunch basis if bunches without seed are accounted for. Pollination success rate increases from smaller to larger bunches of EACBs. Seed set is biased toward the distal third part of fruits of examined EACBs as well tetraploid Matooke hybrid ‘401K-1’ (AAAA) and improved diploid ‘Zebrina’ GF (AA) that were used for comparison. In comparison, in the highly female fertile ‘Calcutta 4,’ seed set is along the entire length of the fruit.Conclusion: Seed set bias in the distal hands and distal end of fruits suggests a systematic mechanism rather than a random occurrence. It is expected that this information will provide a foundation for increased crossbreeding efficiency in bananas.

show abstract

“…cubense causing fusarium wilt or Panama disease [8]. Breeding of resistant/tolerant cultivars is the most sustainable intervention for banana health management [9,10,11]. However, plant breeding is a long process requiring e cient selection of suitable parents with desired traits to produce superior hybrids [12].…”

Section: Introductionmentioning

confidence: 99%

Significant progressive heterobeltiosis in banana crossbreeding

Batte¹,

Nyine

Uwimana³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Heterobeltiosis is the phenomenon when the hybrid’s performance is superior to its best performing parent. Banana ( Musa spp. AAA) breeding is a tedious, time-consuming process, taking up to two decades to develop a consumer acceptable hybrid. Exploiting heterobeltiosis in banana breeding will help to select breeding material with high complementarity, thus increasing banana breeding efficiency. The aim of this study was therefore to determine and document the level of heterobeltiosis of bunch weight and plant stature in the East African highland bananas, in order to identify potential parents that can be used to produce offspring with desired bunch weight and stature after a few crosses.Results: This research found significant progressive heterobeltiosis in cross-bred ‘Matooke’ (highland cooking) banana hybrids, also known as NARITAs, when grown together across years with their parents and grandparents in Uganda. Most (all except 4) NARITAs exhibited positive heterobeltiosis for bunch weight, whereas slightly more than half of them had negative heterobeltiosis for stature. The secondary triploid NARITA 17 had the highest heterobeltiosis for bunch weight: 249% versus its matooke grandparent and 136% against its primary tetraploid parent. Broad sense heritability (across three cropping cycles) for yield potential and bunch weight were high (0.84 and 0.76 respectively), while that of plant stature was very low (0.0035). There was a positive significant correlation ( P < 0.05) between grandparent heterobeltiosis for bunch weight and genetic distance between parents (r = 0.39, P = 0.036), bunch weight (r = 0.7, P < 0.001), plant stature (r = 0.38, P = 0.033) and yield potential (r = 0.59, P < 0.001). Grandparent heterobeltiosis for plant stature was significantly, but negatively, correlated to the genetic distance between parents (r = -0.6, P < 0.001).Conclusions: Such significant heterobeltiosis exhibited for bunch weight is to our knowledge the largest among main food crops. Since bananas are vegetatively propagated, the effect of heterobeltiosis is easily fixed in the hybrids and will not be lost over time after the release and further commercialization of these hybrids.

show abstract

Crossbreeding East African Highland Bananas: Lessons Learnt Relevant to the Botany of the Crop After 21 Years of Genetic Enhancement

Cited by 55 publications

References 22 publications

Seed Set Patterns in East African Cooking Bananas (Musa spp.) are Dependent on Weather Before, During, and After Pollination

Seed Set Patterns in East African Cooking Bananas (Musa spp.) are Dependent on Weather Before, During, and After Pollination

Seed Set Patterns in East African Cooking Bananas are Asymmetric in Bunches and Fruits

Significant progressive heterobeltiosis in banana crossbreeding

Contact Info

Product

Resources

About