Cross-Transmission of Multidrug-ResistantAcinetobacter baumanniiClonal Strains Causing Episodes of Sepsis in a Trauma Intensive Care Unit

Markogiannakis, Antonios; Fildisis, George; Tsiplakou, S.; Ikonomidis, Alexandros; Koutsoukou, A.; Pournaras, Spyros; Manolis, Evangelos N.; Baltopoulos, George; Tsakris, Athanassios

doi:10.1086/533545

Cited by 76 publications

(58 citation statements)

References 28 publications

(23 reference statements)

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“…There have been reports of contaminated units for which complete closure was required to control the outbreaks (16,17). In contrast, a recent study reported that closure of contaminated units was not crucial, as strict contact precautions to control an A. baumannii outbreak were sufficient (18).…”

mentioning

confidence: 99%

Outbreak of Multiresistant OXA-24- and OXA-51-Producing Acinetobacter baumannii in an Internal Medicine Ward

Tena

Martínez

Oteo³

et al. 2013

Jpn J Infect Dis

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SUMMARY:Here we describe the clinical, microbiological, epidemiological, and molecular characterization of an outbreak of multidrug-resistant Acinetobacter baumannii (MRAB) involving 5 patients admitted to the internal medicine ward of our hospital. Over a 6-week period, 5 MRAB isolates were recovered from 5 patients, including 1 with fatal meningitis, 3 with skin and soft tissue infections, and 1 with respiratory colonization. One sample obtained during environmental monitoring in the ward was A. baumannii-positive. According to the pulsed-field gel electrophoresis typing results, the strains isolated from all patients and the environmental sample belonged to a single clone, identified as ST79 by multilocus sequence typing. The bla OXA-24 and bla OXA-51 carbapenemases were detected in all isolates. Four patients died, but only the death of the meningitis patient was probably related to the A. baumannii infection. The infection source was probably the hands of the healthcare workers because the outbreak strain was isolated from the surface of a serum container. The results of the present study revealed the importance of strict adherence to control measures by all healthcare workers because the consequences of noncompliance can be very serious.

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mentioning

confidence: 99%

Outbreak of Multiresistant OXA-24- and OXA-51-Producing Acinetobacter baumannii in an Internal Medicine Ward

Tena

Martínez

Oteo³

et al. 2013

Jpn J Infect Dis

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“…Multi drug resistant A. baumannii is a common challenge in treatment of hospital infections in many countries, including America, Italy, Korea, Greece, Belgium and Iraq (9)(10)(11)(12)(13)(14).The spread on antibiotic resistance among AcJundishapur J Microbiol. 2017; 10(11):e59370.…”

Section: Discussionmentioning

confidence: 99%

“…The above mentioned properties of these species and the emerging antibiotic resistance for A. baumannii, could be related to biofilm formation. Outer membrane proteins (OMPs) are the plentiful surface proteins of this organism cause of nosocomial infections (6,(9)(10)(11)(12)(13)(14)(15). The most important way for restriction of infectious diseases is vaccination.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistant Determination and Prokaryotic Expression of smpA Gene of Acinetobacter baumannii Isolated from Admitted Patients

Ansari

Doosti

Kargar

et al. 2017

Jundishapur J Microbiol

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Background: Acinetobacter baumannii is a leading cause of worldwide nosocomial infections. As many infections are caused by multidrug-resistant strains, the antibiotic-therapy of them has become greatly difficult. Objectives: The present study was aimed to evaluate the antimicrobial susceptibility of A. baumannii as well as cloning and expression of A. baumannii-smpA gene isolated from infectious patients. Methods: Sixty-seven samples were collected from different clinical infections wards especially intensive-care units (ICU). The A. baumannii was identified according to bacteriological standard method. The antibiotic resistance patterns of the isolates were analyzed by the disk diffusion method. The smpA was amplified by Polymerase chain reaction (PCR) following cloning and sub-cloning in T-vector and expression vector, respectively. Colony-PCR, double-digestion and DNA sequencing confirmed that smpA was cloned into the vectors. The expression of rSmpA in IPTG-induced E. coli-DE3 was examined by Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results: Thirty out of 67 isolates (44.78%) were positive for A. baumannii among which, more than 83% were resistance to all generation of Cephalosporins and the least resistance (10%) was observed for Colistin. The smpA-amplicon was 417 bp. Colony-PCR, double-digestion and sequencing showed that the target gene was inserted into the pTZ57RT and pET32a (+), successfully. Obtaining of a~15 KDa band in SDS-PAGE showed the recombinant pET32a/smpA was highly expressed. Conclusions: These results indicated that high resistance to antibiotics among isolates and high conserved of smpA gene at sequence level among A. baumannii strains, therefore it can be used as an antigenic target for generation vaccine against multi drug resistant (MDR) A. baumannii infections.

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“…It has been increasingly implicated in colonization and infection of patient admitted in hospitals [12]. Due to it fluid genome, this bacterium has the ability to attain rapid resistance to most of the commonly employed antimicrobials.…”

Section: Discussionmentioning

confidence: 99%

Successful control of outbreak caused by clonally related extensively-resistant Acinetobacter baumannii in an intensive care unit

Sultan¹

2017

Journal of Microbiology and Infectious Diseases

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Cross-Transmission of Multidrug-ResistantAcinetobacter baumanniiClonal Strains Causing Episodes of Sepsis in a Trauma Intensive Care Unit

Cited by 76 publications

References 28 publications

Outbreak of Multiresistant OXA-24- and OXA-51-Producing <i>Acinetobacter baumannii</i> in an Internal Medicine Ward

Outbreak of Multiresistant OXA-24- and OXA-51-Producing <i>Acinetobacter baumannii</i> in an Internal Medicine Ward

Antimicrobial Resistant Determination and Prokaryotic Expression of smpA Gene of Acinetobacter baumannii Isolated from Admitted Patients

Successful control of outbreak caused by clonally related extensively-resistant Acinetobacter baumannii in an intensive care unit

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