Cross-talk between the RcsCDB and RstAB systems to control STM1485 gene expression in Salmonella Typhimurium during acid-resistance response

Lamberti, Monica; Farizano, Juan V.; López, Fabián E.; Zamora, M. G. Martínez; Pescaretti, María de Las Mercedes; Delgado, Mònica

doi:10.1016/j.biochi.2019.02.005

Cited by 6 publications

(3 citation statements)

References 52 publications

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“… 57–59 RstA contributes to Salmonella virulence by activating the expression of STM1485 (the homolog of asr ). 33 , 60 STM1485 is required to prevent the degradation of Salmonella in SCV and facilitate type III secretion system assembly, promoting intracellular bacterial replication in human epithelial cells and murine macrophages, thus affecting Salmonella virulence. 61 In this study, we found that the deletion of rstAB led to the downregulation of asr gene expression in macrophages and that the deletion of asr gene weakened the acid tolerance and replication ability of LF82 in macrophages.…”

Section: Discussionmentioning

confidence: 99%

Two-component system RstAB promotes the pathogenicity of adherent-invasive Escherichia coli in response to acidic conditions within macrophages

Yao,

Liu,

et al. 2024

Gut Microbes

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Adherent-invasive Escherichia coli (AIEC) strain LF82, isolated from patients with Crohn’s disease, invades gut epithelial cells, and replicates in macrophages contributing to chronic inflammation. In this study, we found that RstAB contributing to the colonization of LF82 in a mouse model of chronic colitis by promoting bacterial replication in macrophages. By comparing the transcriptomes of rstAB mutant- and wild-type when infected macrophages, 83 significant differentially expressed genes in LF82 were identified. And we identified two possible RstA target genes ( csgD and asr ) among the differentially expressed genes. The electrophoretic mobility shift assay and quantitative real-time PCR confirmed that RstA binds to the promoters of csgD and asr and activates their expression. csgD deletion attenuated LF82 intracellular biofilm formation, and asr deletion reduced acid tolerance compared with the wild-type. Acidic pH was shown by quantitative real-time PCR to be the signal sensed by RstAB to activate the expression of csgD and asr . We uncovered a signal transduction pathway whereby LF82, in response to the acidic environment within macrophages, activates transcription of the csgD to promote biofilm formation, and activates transcription of the asr to promote acid tolerance, promoting its replication within macrophages and colonization of the intestine. This finding deepens our understanding of the LF82 replication regulation mechanism in macrophages and offers new perspectives for further studies on AIEC virulence mechanisms.

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Section: Discussionmentioning

confidence: 99%

Two-component system RstAB promotes the pathogenicity of adherent-invasive Escherichia coli in response to acidic conditions within macrophages

Yao,

Liu,

et al. 2024

Gut Microbes

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show abstract

“…Additional studies have identified RstAB-regulated genes by other experimental approaches including overexpression and deletion of rstAB (Cabeza et al, 2007;Ogasawara et al, 2007). In E. coli, RstAB regulates iron acquisition, acid resistance, biofilm formation, adherence and virulence (Oshima et al, 2002;Ogasawara et al, 2007;Ogasawara et al, 2010;Gao et al, 2015;Liu et al, 2019;Gao et al, 2020), whereas some of the functions impacted by RstAB in Salmonella include pyrimidine metabolism, iron acquisition and acid resistance (Tran et al, 2016;Torrez Lamberti et al, 2019). Inactivation of RstAB caused virulence reduction in Yersinia pseudotuberculosis (Flamez et al, 2008), avian pathogenic E. coli (Gao et al, 2015) and Edwardsiella ictaluri (Menanteau-Ledouble and Lawrence, 2013), providing evidence that this regulatory system governs virulence-related functions in diverse species of the Enterobacteriaceae.…”

Section: Discussionmentioning

confidence: 99%

“…In E . coli , RstAB regulates iron acquisition, acid resistance, biofilm formation, adherence and virulence (Oshima et al ., 2002; Ogasawara et al ., 2007; Ogasawara et al ., 2010; Gao et al ., 2015; Liu et al ., 2019; Gao et al ., 2020), whereas some of the functions impacted by RstAB in Salmonella include pyrimidine metabolism, iron acquisition and acid resistance (Tran et al ., 2016; Torrez Lamberti et al ., 2019). Inactivation of RstAB caused virulence reduction in Yersinia pseudotuberculosis (Flamez et al ., 2008), avian pathogenic E .…”

Section: Discussionmentioning

confidence: 99%

The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

Matanza

López-Suárez

Vale

et al. 2021

Environmental Microbiology

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The marine bacterium Photobacterium damselae subsp. damselae (Pdd) causes disease in marine animals and humans. Previous studies demonstrated that mutation of the two-component system RstAB strongly impacts virulence of this pathogen, but the RstAB regulon has not been thoroughly elucidated. We here compared the transcriptomes of Pdd RM-71 and ΔrstA and ΔrstB derivatives using RNA-seq. In accordance with previous studies, RstAB positively regulated cytotoxins Dly, PhlyP and PhlyC. This analysis also demonstrated a positive regulation of outer membrane proteins, resistance against antimicrobials and potential virulence factors by this system. Remarkably, RstAB positively regulated two hitherto uncharacterised gene clusters involved in the synthesis of a polysaccharide capsule. Presence of a capsular layer in wild-type cells was confirmed by transmission electron microscopy, whereas rstA and rstB mutants were non-capsulated. Mutants for capsule synthesis genes, wza and wzc exhibited acapsular phenotypes, were impaired in resistance against the bactericidal action of fish serum and mucus, and were strongly impaired in virulence for fish, indicating a major role of capsule in virulence.Collectively, this study demonstrates that RstAB is a major positive regulator of key virulence factors including a polysaccharide capsule essential for full virulence in a pathogenic Photobacterium.

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LiaSR two-component system modulates the oxidative stress response in Streptococcus mutans

Huang,

Du,

et al. 2023

Microbial Pathogenesis

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Cross-talk between the RcsCDB and RstAB systems to control STM1485 gene expression in Salmonella Typhimurium during acid-resistance response

Cited by 6 publications

References 52 publications

Two-component system RstAB promotes the pathogenicity of adherent-invasive Escherichia coli in response to acidic conditions within macrophages

Two-component system RstAB promotes the pathogenicity of adherent-invasive Escherichia coli in response to acidic conditions within macrophages

The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

LiaSR two-component system modulates the oxidative stress response in Streptococcus mutans

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