Cross-talk between singlet oxygen- and hydrogen peroxide-dependent signaling of stress responses in
            <i>Arabidopsis thaliana</i>

Laloi, Christophe; Stachowiak, Monika; Pers‐Kamczyc, Emilia; Warzych, Ewelina; Murgia, Irene; Apel, Klaus

doi:10.1073/pnas.0609063103

Cited by 292 publications

(250 citation statements)

References 43 publications

(42 reference statements)

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“…The role of 1 O 2 is still not well known as compared with other ROS, but recent findings suggest a function in signaling and its involvement in the response to pathogens (Vellosillo et al, 2010). A cross talk between 1 O 2 -and H 2 O 2 -dependent signaling has been reported to occur during certain environmental stress conditions (Laloi et al, 2007). It is thus possible that 1 O 2 is a major signaling factor of root border-like cells under biotic stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

et al. 2013

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Plant pathogens including fungi and bacteria cause many of the most serious crop diseases. The plant innate immune response is triggered upon recognition of microbe-associated molecular patterns (MAMPs) such as flagellin22 and peptidoglycan. To date, very little is known of MAMP-mediated responses in roots. Root border cells are cells that originate from root caps and are released individually into the rhizosphere. Root tips of Arabidopsis (Arabidopsis thaliana) and flax (Linum usitatissimum) release cells known as "border-like cells." Whereas root border cells of pea (Pisum sativum) are clearly involved in defense against fungal pathogens, the function of border-like cells remains to be established. In this study, we have investigated the responses of root border-like cells of Arabidopsis and flax to flagellin22 and peptidoglycan. We found that both MAMPs triggered a rapid oxidative burst in root border-like cells of both species. The production of reactive oxygen species was accompanied by modifications in the cell wall distribution of extensin epitopes. Extensins are hydroxyproline-rich glycoproteins that can be cross linked by hydrogen peroxide to enhance the mechanical strength of the cell wall. In addition, both MAMPs also caused deposition of callose, a well-known marker of MAMP-elicited defense. Furthermore, flagellin22 induced the overexpression of genes involved in the plant immune response in root border-like cells of Arabidopsis. Our findings demonstrate that root borderlike cells of flax and Arabidopsis are able to perceive an elicitation and activate defense responses. We also show that cell wall extensin is involved in the innate immunity response of root border-like cells.

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Section: Discussionmentioning

confidence: 99%

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

et al. 2013

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“…3C). Additional evidence of a close correlation between 1 O 2 accumulation and cell death in ch1 was obtained by monitoring H 2 O 2 and 1 O 2 -specific marker genes (Gadjev et al, 2006;Laloi et al, 2007). We analyzed the relative expression levels of two H 2 O 2 -responsive genes and two 1 O 2 -responsive genes (Fig.…”

Section: O 2 Generation Induces Cell Death In Arabidopsis Leaf Tissuesmentioning

confidence: 99%

“…All PCR reactions were performed in triplicate on material from two biological repeat experiments. The H 2 O 2 -specific genes used were At1g49150 (unknown protein) and At1g62380 (ACO2), the 1 O 2 -specific genes At5g10830 (embryo abundant protein-like) and At3g61190 (BAP1; Gadjev et al, 2006;Laloi et al, 2007). Universal ProbeLibrary probes (Roche Applied Science) and primers used can be found in Supplemental Table S2.…”

Section: Rna Isolation and Quantitative Reverse Transcription-pcrmentioning

confidence: 99%

Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants

Triantaphylidès¹,

Krischke²,

Hoeberichts³

et al. 2008

Plant Physiology

498

358

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Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate between free radical (type I)-and singlet oxygen ( 1 O 2 ; type II)-mediated lipid peroxidation (LPO) signatures by using hydroxy fatty acids as specific reporters. Using this method, we observed that in nonphotosynthesizing Arabidopsis (Arabidopsis thaliana) tissues, nonenzymatic LPO was almost exclusively catalyzed by free radicals both under normal and oxidative stress conditions. However, in leaf tissues under optimal growth conditions, 1 O 2 was responsible for more than 80% of the nonenzymatic LPO. In Arabidopsis mutants favoring 1 O 2 production, photooxidative stress led to a dramatic increase of 1 O 2 (type II) LPO that preceded cell death. Furthermore, under all conditions and in mutants that favor the production of superoxide and hydrogen peroxide (two sources for type I LPO reactions), plant cell death was nevertheless always preceded by an increase in 1 O 2 -dependent (type II) LPO. Thus, besides triggering a genetic cell death program, as demonstrated previously with the Arabidopsis fluorescent mutant, 1 O 2 plays a major destructive role during the execution of reactive oxygen species-induced cell death in leaf tissues.Plant leaves capture sun-derived light energy to drive CO 2 fixation during photosynthesis. During this process, leaves need to cope with photooxidative stress when the balance between energy absorption and consumption is disturbed. Excess excitation energy in the photosystems (PSI and PSII) leads to the inhibition of photosynthesis via the production of various reactive oxygen species (ROS) at different spatial levels of the cell (Apel and Hirt, 2004;Asada, 2006;Van Breusegem and Dat, 2006). Both exposure to high light intensities and decreased CO 2 availability direct linear electron transfer toward the reduction of molecular oxygen, generating superoxide radicals (O 2 2. ) at PSI (the Mehler reaction). Superoxide dismutation generates hydrogen peroxide (H 2 O 2 ), which is detoxified in the chloroplast by ascorbate peroxidases. As such, this so-called water-water cycle participates in the dissipation of excess energy (Asada, 2006). Decreased CO 2 availability affects the first step in CO 2 fixation by shifting the carboxylation of Rubisco by the Rubisco carboxylase-oxygenase enzyme toward oxygenation, a process called photorespiration. This leads, through the action of glycolate oxidase, to peroxisomal H 2 O 2 production that is counteracted by catalases. Finally, when the intersystem electron carriers are overreduced, triplet excited P680 in the PSII reaction center as well as triplet chlorophylls in the light-harvesting antennae are produced, with the production of singlet oxygen ( 1 O 2 ) as a consequence (Krieger-Liszkay, 2005). In photosynthetic membranes, 1 O 2 ...

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“…In wild-type plants treated with 3-(3,4-dichlorophenyl)-1,1-dimethylurea, an inhibitor of oxidation of the primary quinone electron acceptor of PSII and, consequently, photosynthetic electron transport, the production of 1 O 2 in PSII reaction centers is increased, and in these circumstances, the EXE1/EXE2 pathway promotes cell death (Wagner et al, 2004). The presence of an antagonistic anti-cell death system, involving signaling by the ROS hydrogen peroxide (H 2 O 2 ), has been demonstrated that counteracts the EXE1/EXE2 pro-cell death pathway (Laloi et al, 2007). The H 2 O 2 anti-cell death pathway may control the capacity of the cell to quench 1 O 2 signaling by regulating lipid-soluble antioxidant levels and control of the repair of photodamaged D1 protein, a component of the PSII reaction center.…”

Section: O 2 Signalingmentioning

confidence: 99%

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

2010

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Severe environmental stress imposed on plant tissues induces changes in oxygen (O 2 ) metabolism that cause oxidative stress. Oxidative stress occurs when reactive oxygen species (ROS) are not rapidly scavenged and the rate of repair of damaged cell components fails to keep pace with the rate of damage. If this situation persists, irreversible damage results in a loss of physiological competence and eventual cell death. However, ROS production in leaves resulting from moderate environmental stresses, within the adaptive range of the plant, also has important local and systemic signaling roles. In these circumstances, ROS production induces defense mechanisms that protect the plant but do not result in oxidative stress. We shall illustrate ROS involvement in signaling in both of these situations by considering the role of chloroplasts in initiating cellular responses to environmental perturbations, choosing examples where this organelle interacts with specific signaling pathways.

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Cross-talk between singlet oxygen- and hydrogen peroxide-dependent signaling of stress responses in Arabidopsis thaliana

Abstract: ascorbate peroxidase ͉ chloroplast ͉ oxidative stress ͉ reactive oxygen species

Cited by 292 publications

References 43 publications

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

Deciphering the Responses of Root Border-Like Cells of Arabidopsis and Flax to Pathogen-Derived Elicitors

Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants

Oxidative Stress: Antagonistic Signaling for Acclimation or Cell Death?

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