Cross-talk between neural and immune receptors provides a potential mechanism of homeostatic regulation in the gut mucosa

Assas, Bakri M.; Miyan, Jaleel; Pennock, Joanne L.

doi:10.1038/mi.2014.80

Cited by 42 publications

(39 citation statements)

References 94 publications

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“…TRPV1 is also expressed in the CNS (Szolcsanyi 1988, Mezey et al 2000 and in several non-neuronal cells in the skin, kidneys, lungs, testis, pancreas, spleen, cornea and uterus (Rocha et al 2011, Liu et al 2012, Song et al 2012. Although the physiological/pathophysiological relevance of nonneuronal TRP is unknown, a cross-talk has been proposed between non-neuronal and neuronal TRP channels (Denda et al 2010, Assas et al 2014. Activation of non-neuronal TRPV1 in various cells results in mediator release with both physiological and pathological functions (Fernandes et al 2012): activation of epidermal TRPV1 increases IL8 and prostaglandin E 2 (Southall et al 2003), while TRPA1 induces IL1A and IL1B release (Nilius & Szallasi 2014).…”

Section: Introductionmentioning

confidence: 99%

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Pohóczky

Kun

Szalontai

et al. 2015

Journal of Molecular Endocrinology

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Transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors expressed predominantly in sensory nerves are activated by inflammatory stimuli and mediate inflammation and pain. Although they have been shown in the human endometrium, their regulation and function are unknown. Therefore, we investigated their estrogen-and progesterone-dependent alterations in the rat endometrium in comparison with the estrogenregulated inflammatory cytokine macrophage migration inhibitory factor (MIF). Four-week-old (sexually immature) and four-month-old (sexually mature) female rats were treated with the non-selective estrogen receptor (ER) agonist diethylstilboestrol (DES), progesterone and their combination, or ovariectomized. RT-PCR and immunohistochemistry were performed to determine mRNA and protein expression levels respectively. Channel function was investigated with ratiometric [Ca 2C ] i measurement in cultured primary rat endometrial cells. Both TRP receptors and MIF were detected in the endometrium at mRNA and protein levels, and their localizations were similar. Immunostaining was observed in the immature epithelium, while stromal, glandular and epithelial positivity were observed in adults. Functionally active TRP receptor proteins were shown in endometrial cells by activation-induced calcium influx. In adults, Trpa1 and Trpv1 mRNA levels were significantly up-regulated after DES treatment. TRPA1 increased after every treatment, but TRPV1 remained unchanged following the combined treatment and ovariectomy. In immature rats, DES treatment resulted in increased mRNA expression of both channels and elevated TRPV1 immunopositivity. MIF expression changed in parallel with TRPA1/TRPV1 in most cases. DES up-regulated Trpa1, Trpv1 and Mif mRNA levels in endometrial cell cultures, but 17b-oestradiol having ERa-selective potency increased only the expression of Trpv1. We provide the first evidence for TRPA1/TRPV1 expression and their estrogen-induced up-regulation in the rat endometrium in correlation with the MIF. Journal of Molecular EndocrinologyResearch K POHÓ CZKY and others TRPV1 and TRPA1 in the rat endometrium 56:2 135-149

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Section: Introductionmentioning

confidence: 99%

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Pohóczky

Kun

Szalontai

et al. 2015

Journal of Molecular Endocrinology

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“…Lundy dan Linden 8 membahas lebih dalam konsep interaksi keradangan tersebut yang dapat terjadi di dalam rongga mulut. Hipotesa tersebut sudah banyak dibuktikan sehingga dianggap sebagai mekanisme yang telah mapan, 9 sehingga dapat menerangkan penjalaran gingivitis ke jaringan lain dan menjelaskan pengaruh ADT terhadap biomarker migren. Biomarker yang terlibat dalam neurogenic switching dan juga migren adalah TNF-α, merupakan produk berbagai sel imun, penyebab nyeri dan juga dapat merangsang ujung saraf aferen, 2 leukotrien C4 (LTC4) yang menunjukkan aktivitas sel mast dan penyebab nyeri, 10 serta substans P (SP) yang selain menunjukkan aktivitas sel saraf juga dapat merangsang degranulasi sel mast.…”

Section: Dr Meggsunclassified

Menurunkan Biomarker Migren Secara Cepat dengan Terapi “Assisted Drainage” (Studi Eksperimental pada Hewan coba)

Utomo

2015

Majalah Kedokteran Gigi Indonesia

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ABSTRAKMigren merupakan gejala nyeri kepala rekuren yang paling sering dikeluhkan dalam dunia medis. Di Amerika Serikat, lebih dari 30 juta orang menderita satu atau lebih nyeri kepala migren dalam setahun. Berdasarkan literatur, migren merupakan komorbiditas rinosinusitis dan asma serta melibatkan saraf trigeminus. Tatalaksana migren dalam bidang kedokteran adalah obat-obatan dan tindakan invasif seperti injeksi atau operasi pada saraf. Sangat menarik bahwa menurut laporan kasus telah dibuktikan bahwa migren berhubungan dengan gingivitis karena suatu perawatan periodontal non invasif yang disebut sebagai terapi "assisted drainage" (ADT) dapat mengurangi gejala migren dalam hitungan menit. Terapi tersebut berupa masase sulkus gingiva dalam 3 menit dengan sisi tumpul scaler manual sampai timbul keluarnya darah secara pasif. Akan tetapi mekanisme kejadian pengurangan gejala migren setelah ADT masih belum jelas. Tujuan penelitian adalah untuk verifikasi pengaruh ADT terhadap penurunan biomarker migren tikus nonalergi dan alergi yang gingivitis. Tikus Wistar dibagi menjadi dua kelompok secara acak, penelitian dengan control series design study. Kelompok perlakuan adalah tikus alergi yang diinduksi dengan ovalbumin (OVA) dan non-alergi, kelompok ini disuntik dengan lipopolisakarida Porphyromonas gingivalis. Kelompok kontrol positif adalah tikus alergi, kontrol negatif dengan phosphate buffered saline (PBS). Pada beberapa kelompok dilakukan ADT. Ekspresi substans P (SP), leukotrien C4 (LTC4) dan TNF α pada gingiva dan hidung diperiksa dengan imunohistokimia peroksidase. PENDAHULUANMigren adalah nyeri kepala neurovaskuler kambuhan yang ditandai dengan nyeri kepala berdenyut parah berkala, disertai mual, muntah, fotofobia, fonofobia, enggan melakukan aktivitas fisik. Prevalensi migren meningkat dari 4% sebe lum pubertas sampai pada puncaknya, yaitu 25% pada wanita usia subur serta berkurang setelah menopause. Kurang lebih 30 juta penduduk Amerika Serikat dewasa (18% wanita dan 6% of pria) menderita migren. ARTIKEL PENELITIAN

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“…TRPV1 has been linked to the crosstalk between the microbiota and the neuro-immune response in the gut, because TRPV1 and CGRP can modulate cytokine response to lipopolysaccharide (LPS) independently of the adaptive immune response. It has been proposed that TLR4 can activate TRPV1 via intracellular signalling thereby inducing the subsequent release of anti-inflammatory CGRP to maintain mucosal homeostasis [104]. In addition, blocking of TRPV4 has also been shown to alleviate colitis and pain associated with the intestinal inflammation induced by TNBS in mice [105].…”

Section: The Intestinal Vanilloid Systemmentioning

confidence: 99%

“…In particular, TLR2, 4 and 7 have been directly implicated in the modulation of nociceptive markers and visceral hypersensitivity and pain [72,104,210,[216][217][218][219][220][221]. It has also been proposed that a neurochemical 'delivery system' exists whereby gut bacteria can send messages to the brain.…”

Section: Microbiota-gut-brain Axis and Ibdmentioning

confidence: 99%

Microbial Neuro-Immune Interactions and the Pathophysiology of IBD

Aguilera¹,

Melgar²

2016

New Insights Into Inflammatory Bowel Disease

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Inflammatory bowel disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC), is a group of debilitating disorders affecting patient's quality of life and with unknown aetiology. The collected evidence indicates that individuals can develop IBD as a result of genetic susceptibility, a dysregulated immune response and the influence of certain environmental factors. Common symptomatology includes abdominal pain, fever and bowel diarrhoea with blood and/or mucus excretion. The location and extent of disease differ between UC and CD, affecting the mucosal layer in the colon in UC patients, whereas in CD patients, a transmural inflammation is found anywhere in the gastrointestinal tract. Factors associated with IBD pathophysiology include alterations in immune responses, characterized by an atypically T helper (Th)-2 profile in UC, and a Th1/Th17 profile in CD, modifications in epithelial barrier function and alterations in the commensal microbiota composition with blooming of specific pathobionts, for example, adherent-invasive Escherichia coli (AIEC), and with diet. Recent research has uncovered that inflammation, per se, can activate the enteric nervous system inducing neurogenic inflammation and increasing visceral sensitivity, leading to pain. Similarly, alterations in the commensal microbiota composition/ligands have also led to modifications in intestinal nociceptive markers and in visceral pain. In this chapter, we aim to review the mechanisms implicated in microbial neuroimmune axis and its potential contribution to IBD pathophysiology and symptomatology. We focus on the findings identified in animal models and in IBD patients and on the prospective translation of targeting the microbial neuro-immune axis as future therapeutic treatment for intestinal inflammatory conditions.

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Cross-talk between neural and immune receptors provides a potential mechanism of homeostatic regulation in the gut mucosa

Cited by 42 publications

References 94 publications

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Estrogen-dependent up-regulation of TRPA1 and TRPV1 receptor proteins in the rat endometrium

Menurunkan Biomarker Migren Secara Cepat dengan Terapi “Assisted Drainage” (Studi Eksperimental pada Hewan coba)

Microbial Neuro-Immune Interactions and the Pathophysiology of IBD

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