Cross-species association of quail invariant chain with chicken and mouse MHC II molecules

Chen, Fangfang; Wu, Chao; Pan, Ling; Xu, Fazhi; Liu, Xuelan; Yu, Weiwu

doi:10.1016/j.dci.2013.01.002

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…In avian species, two isoforms of CD74, p31 and p41 were characterized in the chicken (Bremnes et al, 2000;Zhong et al, 2004), duck (Zhong et al, 2006), goose (Chen et al, 2011), and quail (Chen et al, 2013). Avian CD74p41 contains a transmembrane (TM) and class II-associated invariant chain peptide (CLIP) as well as a thyroglobulin type-1 repeat (Tg), while CD74p31 lacks the Tg domain at the C-terminus.…”

Section: Discussionmentioning

confidence: 99%

Both host and parasite MIF molecules bind to chicken macrophages via CD74 surface receptor

Kim

Cox

Jenkins

et al. 2014

Developmental & Comparative Immunology

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Section: Discussionmentioning

confidence: 99%

Both host and parasite MIF molecules bind to chicken macrophages via CD74 surface receptor

Kim

Cox

Jenkins

et al. 2014

Developmental & Comparative Immunology

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“…Moreover, previous studies showed that CD74 is involved as a signaling molecule in B cell maturation (37) and MHC-I expression on the cell surface (38). Chicken CD74 (MHC-II Ii) exists in two isoforms (Ii-1 and Ii-2), resulting from alternative splicing, and is strongly expressed in the major immune organs (31,32). Northern blot results in 6-week-old chickens revealed high levels of Ii-1 and Ii-2 mRNA expression in the bursa and spleen and low levels of Ii-1 in the heart, liver, and thymus, while Ii-2 was not expressed in these tissues (31).…”

Section: Discussionmentioning

confidence: 99%

“…At present, two isoforms of chicken CD74 have been uncovered: Ii-1 and Ii-2 (corresponding to human p33 and p41, respectively) (31). Ii-1 has the function of enhancing the presentation of Ii-dependent MHC-II-restricted epitopes (31), while Ii-2 could act as a regulatory factor in antigen presentation epitopes (32). Moreover, CD74 is the cellular receptor of the macrophage migrationinhibitory factor (MIF), participating in the activation of downstream inflammatory pathways (33).…”

mentioning

confidence: 99%

Identification of Chicken CD74 as a Novel Cellular Attachment Receptor for Infectious Bursal Disease Virus in Bursa B Lymphocytes

Liu

Pan

et al. 2020

J Virol

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Infectious bursal disease virus (IBDV) is an important member of the Birnaviridae family, causing severe immunosuppressive disease in chickens. The major capsid protein VP2 is responsible for the binding of IBDV to the host cell and its cellular tropism. In order to find proteins that potentially interact with IBDV VP2, a liquid chromatography-mass spectrometry (LC-MS) assay was conducted, and the host chicken CD74 protein was identified. Here, we investigate the role of chicken CD74 in IBDV attachment. Coimmunoprecipitation assays indicated that the extracellular domain of CD74 interacted with the VP2 proteins of multiple IBDV strains. Knockdown and overexpression experiments showed that CD74 promotes viral infectivity. Confocal assays showed that CD74 overexpression allows the attachment of IBDV and subvirus-like particles (SVPs) to the cell surface of nonpermissive cells, and quantitative PCR (qPCR) analysis further confirmed the attachment function of CD74. Anti-CD74 antibody, soluble CD74, depletion of CD74 by small interfering RNA (siRNA), and CD74 knockdown in the IBDV-susceptible DT40 cell line significantly inhibited IBDV binding, suggesting a pivotal role of this protein in virus attachment. These findings demonstrate that CD74 is a novel important receptor for IBDV attachment to the chicken B lymphocyte cell line DT40. IMPORTANCE CD74 plays a pivotal role in the correct folding and functional stability of major histocompatibility complex class II (MHC-II) molecules and in the presentation of antigenic peptides, acting as a regulatory factor in the antigen presentation process. In our study, we demonstrate a novel role of CD74 during IBDV infection, showing that chicken CD74 plays a significant role in IBDV binding to target B cells by interacting with the viral VP2 protein. This is the first report demonstrating that CD74 is involved as a novel attachment receptor in the IBDV life cycle in target B cells, thus contributing new insight into host-pathogen interactions.

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“…p41 also consistently contains conserved Tg domains, which are remarkably similar each other on the amino acid sequence level and in their molecular structures in various species (Zhong et al, 2006;Chen et al, 2013). Ii in Muscovy Duck (MDIi) might contains these 2 isoforms, MDIi-1 [MDIi in reference (Liu et al, 2011), GenBank accession No.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

Liu¹,

Chen²,

Wu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Invariant chain (Ii) isoform, through its thyroglobulin-like (Tg) domain, inhibits cysteine proteases during antigen presentation in vertebrates. In birds, the Ii of Muscovy Duck (MDIi) has 2 forms: MDIi-1 and MDIi-2 (MDIi isoform). To understand the genetic information and expression characteristics of MDIi-2, polymerase chain reaction, and bioinformatic analysis were performed for MDIi-2 from healthy adult Muscovy Duck. The full-length MDIi-2 cDNA sequence was found to be 1377-base pairs, encoding a 285-amino acid protein. MDIi-2 contains 63 amino acids with an insertion sequence in the Tg domain. MDIi-2 shares high identity (72.51-94.74%) with the same protein in other birds. The Tg domain of MDIi-2 is highly conserved and showed relatively high identity (96.83%) among all tested birds. The molecular structure of the Tg domain supports this conservation. MDIi-2 expression was measured in various tissues using real-time quantitative polymerase chain reaction. Similar to MDIi-1, MDIi-2 was detected in all tissues but at different levels. Higher expression level was observed in the spleen, intestinal mucosa, and bursa stipe (bursa of Fabricius stipe) than in other tissues. This suggests that MDIi-2, like MDIi-1, plays an essential role in all tissues and that its differential expression may be related to its functions in these tissues. The coexistence of 2 MDIi isoforms indicates that their functions are correlated in Muscovy Duck. This study improves the understanding of poultry immunology and may be used to improve measures to protect Muscovy Duck from disease.

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Cross-species association of quail invariant chain with chicken and mouse MHC II molecules

Cited by 6 publications

References 33 publications

Both host and parasite MIF molecules bind to chicken macrophages via CD74 surface receptor

Both host and parasite MIF molecules bind to chicken macrophages via CD74 surface receptor

Identification of Chicken CD74 as a Novel Cellular Attachment Receptor for Infectious Bursal Disease Virus in Bursa B Lymphocytes

Molecular characterization and tissue-specific expression of invariant chain isoform in Muscovy Duck (Cairina moschata)

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