Cross-Sectional Study on Prevalence and Molecular Characteristics of Plasmid Mediated ESBL/AmpC-Producing Escherichia coli Isolated from Veal Calves at Slaughter

Hordijk, Joost; Wagenaar, Jaap A.; Kant, A.; Essen-Zandbergen, Alieda van; Dierikx, Cindy; Veldman, Kees; Wit, Ben; Mevius, Dik

doi:10.1371/journal.pone.0065681

Cited by 43 publications

(28 citation statements)

References 44 publications

(59 reference statements)

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“…The predominant resistance gene reported in the present study is bla CTX-M-1 . The high abundance of this gene is consistent with studies on isolates from humans and farm animals in the Netherlands, in which bla CTX-M-1 is either the most or second most predominant gene (Dierikx et al, 2012a; Voets et al, 2012; Hordijk et al, 2013; Platteel et al, 2013). However, more data on plasmids and host bacteria are required to draw any conclusions on genetic relatedness and possible transmission to or from either humans or food-producing animals.…”

Section: Discussionsupporting

confidence: 86%

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

Hordijk¹,

Schoormans²,

Kwakernaak³

et al. 2013

Front. Microbiol.

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201

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Extended-spectrum-β-lactamase (ESBL)/AmpC producing Enterobacteriaceae have been reported worldwide amongst isolates obtained from humans, food-producing animals, companion animals, and environmental sources. However, data on prevalence of fecal carriage of ESBL/AmpC producing Enterobacteriaceae in healthy companion animals is limited. This pilot study describes the prevalence of ESBL/AmpC encoding genes in healthy cats and dogs, and cats and dogs with diarrhea. Twenty fecal samples of each group were cultured on MacConkey agar supplemented with 1 mg/L cefotaxime and in LB-enrichment broth supplemented with 1 mg/L cefotaxime, which was subsequently inoculated on MacConkey agar supplemented with 1 mg/L cefotaxime. ESBL/AmpC genes were identified using the Check-Points CT103 micro array kit and subsequently by sequencing analysis. Chromosomal ampC promoter mutations were detected by PCR and sequencing analysis. From the healthy and diarrheic dogs, respectively 45 and 55% were positive for Escherichia coli with reduced susceptibility for cefotaxime. From the healthy and diarrheic cats, the estimated prevalence was respectively 0 and 25%. One diarrheic cat was positive for both reduced susceptible E. coli and Proteus mirabilis. The ESBL/AmpC genes found in this study were mainly blaCTX-M-1, but also blaCTX-M-14, blaCTX-M-15, blaTEM-52-StPaul, blaSHV-12, and blaCMY-2 were detected. This pilot study showed that the prevalence of ESBL/AmpC producing Enterobacteriaceae in healthy and diarrheic dogs, and diarrheic cats was relatively high. Furthermore, the genes found were similar to those found in isolates of both human and food-producing animal origin. However, since the size of this study was relatively small, extrapolation of the data to the general population of cats and dogs should be done with great care.

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Section: Discussionsupporting

confidence: 86%

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

Hordijk¹,

Schoormans²,

Kwakernaak³

et al. 2013

Front. Microbiol.

Self Cite

201

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“…The presence of ESBL genes and plasmidmediated quinolone resistance (PMQR) genes was confirmed by PCR and sequencing using previously described primers (4). Plasmid DNA was purified with a modified miniprep method (13) and transferred into DH10B cells (Gibco Invitrogen, USA) by electroporation. Subsequently, transformants were cultured on LB agar with 1 mg/liter cefotaxime for selection of ESBL-positive transformants or cultured on LB agar with 0.03 mg/liter ciprofloxacin for selection of PMQR-positive transformants.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Veldman

Tulden

Kant

et al. 2013

Appl Environ Microbiol

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bCloacal swabs from carcasses of Dutch wild birds obtained in 2010 and 2011 were selectively cultured on media with cefotaxime to screen for the presence of extended-spectrum ␤-lactamase (ESBL)/AmpC-producing Escherichia coli. Subsequently, all cefotaxime-resistant E. coli isolates were tested by broth microdilution and microarray. The presence of ESBL/AmpC and coexisting plasmid-mediated quinolone resistance (PMQR) genes was confirmed by PCR and sequencing. To determine the size of plasmids and the location of ESBL and PMQR genes, S1 pulsed-field gel electrophoresis (PFGE) was performed on transformants, followed by Southern blot hybridization. The study included 414 cloacal swabs originating from 55 different bird species. Cefotaxime-resistant E. coli isolates were identified in 65 birds (15.7%) from 21 different species. In all, 65 cefotaxime-resistant E. coli ESBL/AmpC genes were detected, mainly comprising variants of bla CTX-M and bla CMY-2 . Furthermore, PMQR genes [aac(6=)-lb-cr, qnrB1, and qnrS1] coincided in seven cefotaxime-resistant E. coli isolates. Overall, replicon typing of the ESBL/AmpC-carrying plasmids demonstrated the predominant presence of IncI1 (n ‫؍‬ 31) and variants of IncF (n ‫؍‬ 18). Our results indicate a wide dissemination of ESBL and AmpC genes in wild birds from The Netherlands, especially among aquatic-associated species (waterfowl, gulls, and waders). The identified genes and plasmids reflect the genes found predominantly in livestock animals as well as in humans.

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“…Plasmid transformation and conjugation were not successful in transferring the bla OXA48 -like genes from Shewanella spp. to Escherichia coli K-12 recipients, suggesting a chromosomal localization (18,19). The genetic context of bla OXA-48 -like genes was investigated by PCR using primer pairs oxa48b-Fw (5=-AGCTTGATCGCCCTCGATTT-23=) and lysR-Rev (5=-CGGATAGCCATTCCGGTCTC-3=) and oxa48b-Rev (5=-TGATTTGCTCAGT GGCCGAA-3=) and c15-Fw (5=-AAGCGTACTGGGATCATGGC-3=) designed on the genome sequence of S. xiamenensis S4 (GenBank accession no.…”

mentioning

confidence: 97%

Chromosome-Based bla _OXA-48 -Like Variants in Shewanella Species Isolates from Food-Producing Animals, Fish, and the Aquatic Environment

Ceccarelli

Essen-Zandbergen

Veldman

et al. 2017

Antimicrob Agents Chemother

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Carbapenems are considered last-resort antibiotics in health care. Increasing reports of carbapenemase-producing bacteria in food-producing animals and in the environment indicate the importance of this phenomenon in public health. Surveillance for carbapenemase genes and carbapenemase-producing bacteria in Dutch food-producing animals, environmental freshwater, and imported ornamental fish revealed several chromosome-based bla OXA-48 -like variants in Shewanella spp., including two new alleles, bla OXA-514 and bla . Carbapenemase genes were not associated with mobile genetic elements or Enterobacteriaceae.

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Cross-Sectional Study on Prevalence and Molecular Characteristics of Plasmid Mediated ESBL/AmpC-Producing Escherichia coli Isolated from Veal Calves at Slaughter

Cited by 43 publications

References 44 publications

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Chromosome-Based bla _OXA-48 -Like Variants in Shewanella Species Isolates from Food-Producing Animals, Fish, and the Aquatic Environment

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Cross-Sectional Study on Prevalence and Molecular Characteristics of Plasmid Mediated ESBL/AmpC-Producing Escherichia coli Isolated from Veal Calves at Slaughter

Cited by 43 publications

References 44 publications

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

High prevalence of fecal carriage of extended spectrum β-lactamase/AmpC-producing Enterobacteriaceae in cats and dogs

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Chromosome-Based bla OXA-48 -Like Variants in Shewanella Species Isolates from Food-Producing Animals, Fish, and the Aquatic Environment

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Chromosome-Based bla _OXA-48 -Like Variants in Shewanella Species Isolates from Food-Producing Animals, Fish, and the Aquatic Environment