Cross-Sectional Patterns of Hepatobiliary Abnormalities and Possible Precursor Conditions of Cholangiocarcinoma Associated with Opisthorchis viverrini Infection in Humans

Elkins, D. B.; Mairiang, Eimorn; Sithithaworn, Paiboon; Mairiang, Pisaln; Chaiyakum, Jitjaroen; Chamadol, Nittaya; Loapaiboon, Vallop; Haswell‐Elkins, Melissa

doi:10.4269/ajtmh.1996.55.295

Cited by 72 publications

(37 citation statements)

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“…Although there is no standard guideline, several ultrasonographic features of opisthorchiasis including hepatomegaly, periductal fibrosis, gallstones, gallbladder sludge, intrahepatic duct stones and poor functioning gall bladder have been described over the past 20 years or so [8–10]. Periductal fibrosis is a prominent sonographic finding in chronic opisthorchiasis.…”

Section: Discussionmentioning

confidence: 99%

“…Previous community-based ultrasound studies in this opisthorchiasis endemic region of Khon Kaen and neighboring provinces showed a wide range of hepatobiliary abnormalities including periductal fibrosis, cholecystitis, gallstones, and suspected CCA [8–10]. After large scale praziquantel treatment for opisthorchiasis in Khon Kaen province and other parts of northeast Thailand for decades [2], still we frequently encounter the hepatobiliary abnormalities and CCA associated with O. viverrini infection.…”

Section: Introductionmentioning

confidence: 99%

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Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with Opisthorchis viverrini infection in endemic areas of Thailand

Mairiang

Laha

Bethony

et al. 2012

Parasitology International

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106

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A cross sectional study on hepatobiliary abnormalities in opisthorchiasis was performed in 8,936 males and females aged from 20 to 60 years from 90 villages of Khon Kaen province, Northeast Thailand. All were stool-examined for Opisthorchis viverrini infection by standard quantitative formalin/ethyl acetate concentration technique. Of these, 3,359 participants with stool egg positive were underwent ultrasonography of the upper abdomen. The hepatobiliary abnormalities detected by ultrasound are described here. This study found a significantly higher frequency of advanced periductal fibrosis in persons with chronic opisthorchiasis (23.6%), particularly in males. Risks of the fibrosis included intensity of infection, and age younger than 30 years. Height of left lobe of the liver, cross-section of the gallbladder dimensions post fatty meal, sludge, and, interestingly, intrahepatic duct stones were significantly associated with the advanced periductal fibrosis. Eleven suspected cholangiocarcinoma (CCA) cases were observed. This study emphasizes the current status of high O. viverrini infection rate and the existence of hepatobiliary abnormalities including suspected CCA in opisthorchiasis endemic areas of Thailand.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with Opisthorchis viverrini infection in endemic areas of Thailand

Mairiang

Laha

Bethony

et al. 2012

Parasitology International

Self Cite

106

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“…This agrees with earlier findings from Thailand (Pungpak et al, 1989). However, recent community-based ultrasonographic studies showed that hepatobiliar abnormalities are associated with an O. viverrini infection and are possible precursor conditions for cholangiocarcinoma (Elkins et al, 1996).…”

Section: Prevalence and Impact Of Opisthorchis Viverrinimentioning

confidence: 99%

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Sayasone

Odermatt

Phoumindr

et al. 2007

Transactions of the Royal Society of Tropical Medicine and Hygi

128

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a v a i l a b l e a t w w w . s c i e n c e d i r e c t . c o m j o u r n a l h o m e p a g e : w w w . e l s e v i e r h e a l t h . c o m / j o u r n a l s / t r s t Summary In Lao PDR, detailed investigations on Opisthorchis viverrini are scarce. The objective was to document epidemiological features of O. viverrini infections in a highly endemic district. A random sample was selected (13 villages, 15 households/village, all household members aged >6 months). Clinical examinations, short interviews and stool examinations (Kato-Katz technique) were performed. Fish samples were dissected for the presence of metacercaria. In total, 814 persons were enrolled (median age 16 years, 51.5% women). The prevalence was 58.5%. Infection rates increased with age (from 20.0 to 85.5%; P < 0.001). Intensity of infection and the habit of consuming insufficiently cooked fish also increased with age. Of the study participants, 75.2% reported cooking fish insufficiently. Of the 23 different species of cyprinoid fish consumed in the study villages, 20 species were infected. At the village level, the prevalence of raw fish consumption was strongly associated with the infection status of O. viverrini (r = 0.76, P = 0.003). At individual level, age, the consumption of insufficiently cooked fish, and the absence of sanitation were strongly associated. The disease associated with O. viverrini infection needs to be assessed in order to develop and conduct adequate interventions. Epidemiology of

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“…(Cancer Sci 2010; 101: 658-665) C holangiocarcinoma (CCA), the malignant tumor arising from the bile duct epithelium, is the most common cancer in Thailand, especially in the northeast. There is strong epidemiological evidence (1)(2)(3) and experimental studies (4)(5)(6) that support an important etiological role for liver fluke (Opisthorchis viverrini [Ov]) infection in the development of CCA in humans. There is accumulating evidence that Ov infection enhances the risk of CCA via chronic inflammation.…”

mentioning

confidence: 99%

Myristoylated alanine‐rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C‐dependent pathway

et al. 2010

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Myristoylated alanine-rich C kinase substrate (MARCKS), a substrate of protein kinase C (PKC) has been suggested to be implicated in cell adhesion, secretion, and motility through the regulation of the actin cytoskeletal structure. The quantitative real-time-polymerase chain reaction analysis revealed that MARCKS is significantly overexpressed in Opisthorchis viverriniassociated cholangiocarcinoma (CCA) (P = 0.001) in a hamster model, which correlated with the results of mRNA in situ hybridization. An immunohistochemical analysis of 60 CCA patients revealed a significant increase of MARCKS expression. Moreover, the log-rank analysis indicated that CCA patients with a high MARCKS expression have significantly shorter survival times than those with a low MARCKS expression (P = 0.02). This study investigated whether MARCKS overexpression is associated with CCA metastasis. Using a confocal microscopic analysis of CCA cell lines that had been stimulated with the PKC activator, 12-0-tetradecanoyl phorbol-13-acetate (TPA), MARCKS was found to be translocated from the plasma membrane to the perinuclear area. In addition, phosphorylated MARCKS (pMARCKS) became highly concentrated in the perinuclear area. Moreover, an adhesion assay demonstrated that the exogenous overexpression of MARCKS remarkably promoted cell attachment. Interestingly, after TPA stimulation, the CCA cell line-depleted MARCKS showed a decrease in migration and invasion activity. It can be concluded that in non-stimulation, MARCKS promotes cell attachment to the extracellular matrix. After TPA stimulation, PKC phosphorylates MARCKS leading to cell migration or invasion. Taken together, the results of this study reveal a prominent role for MARCKS as one of the key players in the migration of CCA cells and suggest that cycling between MARCKS and pMARCKS can regulate the metastasis of biliary cancer cells. (Cancer Sci 2010; 101: 658-665) C holangiocarcinoma (CCA), the malignant tumor arising from the bile duct epithelium, is the most common cancer in Thailand, especially in the northeast. There is strong epidemiological evidence (1)(2)(3) and experimental studies (4)(5)(6) that support an important etiological role for liver fluke (Opisthorchis viverrini [Ov]) infection in the development of CCA in humans. There is accumulating evidence that Ov infection enhances the risk of CCA via chronic inflammation. (7,8) Recently, Pinlaor et al. (9) suggested that oxidative and nitrosative damage to DNA in the liver of Ov-infected hamsters can play a key role in the modulation of gene expression, which enhances the effect of the DNA adduct and can drive a normal cell undergo tumor development. Additionally, Thanan et al. (10) reported that the highest 8-oxodG level, which is the biomarker of DNA damage, was observed for CCA patients, and a higher level was found in the urine and leukocytes of Ov-infected patients than that in healthy patients.The gene expression profiles of Ov-associated CCA tumors, as investigated by Loilome et al.(11) , indicated that myristoyla...

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Cross-Sectional Patterns of Hepatobiliary Abnormalities and Possible Precursor Conditions of Cholangiocarcinoma Associated with Opisthorchis viverrini Infection in Humans

Cited by 72 publications

References 0 publications

Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with Opisthorchis viverrini infection in endemic areas of Thailand

Ultrasonography assessment of hepatobiliary abnormalities in 3359 subjects with Opisthorchis viverrini infection in endemic areas of Thailand

Epidemiology of Opisthorchis viverrini in a rural district of southern Lao PDR

Myristoylated alanine‐rich C kinase substrate phosphorylation promotes cholangiocarcinoma cell migration and metastasis via the protein kinase C‐dependent pathway

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