Cross-resistance patterns to ACCase-inhibitors in American sloughgrass (Beckmannia syzigachne Steud.) homozygous for specific ACCase mutations

Du, Long; Liu, Weitang; Yuan, Guohui; Guo, Wenlei; Li, Qi

doi:10.1016/j.pestbp.2015.07.005

Cited by 24 publications

(25 citation statements)

References 35 publications

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“…Herbicide dose-response experiments confirmed significant differences in the resistance patterns of homozygous mutant ACCase plants and wild-type plants within the same original field population (Du et al, 2016). All three subpopulations segregating for specific ACCase mutation conferred resistance to fenoxaprop-p-ethyl, while all three subpopulations of derived wild-type genotypes were susceptible to fenoxaprop-p-ethyl (Du et al, 2016). Each two subpopulations (e.g.…”

Section: Genotypes and Phenotypes Of Seeds At The Accase Locusmentioning

confidence: 65%

“…Result confirmed that all seeds homozygous for specific mutant ACCase (1781Leu/Leu, 2027Cys/Cys and 2041Asn/Asn hereafter referred to as 1781M/M, 2027M/M and 2041M/M, respectively) or wild-type ACCase (1781Ile/Ile, 2027Trp/ Trp and 2041Ile/Ile hereafter referred to as 1781W/W, 2027W/W and 2041W/W, respectively), respectively. Herbicide dose-response experiments confirmed significant differences in the resistance patterns of homozygous mutant ACCase plants and wild-type plants within the same original field population (Du et al, 2016). All three subpopulations segregating for specific ACCase mutation conferred resistance to fenoxaprop-p-ethyl, while all three subpopulations of derived wild-type genotypes were susceptible to fenoxaprop-p-ethyl (Du et al, 2016).…”

Section: Genotypes and Phenotypes Of Seeds At The Accase Locusmentioning

confidence: 68%

“…In order to make the genetic background of wild-type (W/W) and homozygous mutant seeds (M/M) similar as much as possible, we used batches of American slough grass seeds that shared a genetic background but were wild-type (W/W) and homozygous mutant (M/M) at the ACCase locus. The seed batches were the same as those used to establish the cross-resistance patterns associated with Ile1781Leu, Trp2027Cys and Ile2041Asn ACCase gene mutations in American slough grass (Du et al, 2016).…”

Section: Plant Materialsmentioning

confidence: 99%

“…Field samples were collected at three different locations in China and then were analyzed by ACCase genotyping and sequencing (Du et al, 2016). Only one mother plant heterozygous (W/M) for one specific mutant ACCase allele and contained no other known mutant ACCase alleles was used to produce subpopulation within one field sample.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In most reported cases, the major mechanism of evolved resistance is ACCase gene mutation leading to amino acid substitutions. To date, 13 distinct resistance-endowing amino acid substitutions located at seven conserved amino acid residues in CT domain of the ACCase gene have been reported (Du et al, 2016). It is often alleged that genes conferring adaption to a new environment often have negative pleiotropic effects on fitness in the absence of the selective pressure in the whole plant life cycle (Purrington, 2000;Strauss et al, 2002;Vila-Aiub et al, 2009), and this has been strongly confirmed in artificial mutants of Arabidopsis thaliana with a homogenized genetic background (Roux et al, 2004).…”

Section: Introductionmentioning

confidence: 97%

See 4 more Smart Citations

Effect of herbicide resistance endowing three ACCase mutations on seed germination and viability in American slough grass ( Beckmannia syzigachne Steud. Fernald)

Du¹,

Bai

et al. 2017

Chilean J. Agric. Res.

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Section: Genotypes and Phenotypes Of Seeds At The Accase Locusmentioning

confidence: 65%

Section: Genotypes and Phenotypes Of Seeds At The Accase Locusmentioning

confidence: 68%

Section: Plant Materialsmentioning

confidence: 99%

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 3 more Smart Citations

Effect of herbicide resistance endowing three ACCase mutations on seed germination and viability in American slough grass ( Beckmannia syzigachne Steud. Fernald)

Du¹,

Bai

et al. 2017

Chilean J. Agric. Res.

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Fitness costs associated with acetyl‐coenzyme A carboxylase mutations endowing herbicide resistance in American sloughgrass (Beckmannia syzigachne Steud.)

Du¹,

Qu²,

Jiang³

et al. 2019

Ecology and Evolution

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Weed resistance to herbicide can be conferred by gene mutations, and some mutations can cause pleiotropic effects in some cases. We investigated the pleiotropic effects associated with five specific ACCase mutations (Ile1781Leu, Trp2027Cys, Ile2041Asn, Asp2078Gly, and Gly2096Ala) on the plant growth, seed production, and resource competitiveness in American sloughgrass. Resistant plants (M/M) homozygous for specific ACCase mutation and susceptible wild‐type plants (W/W) were derived from single heterozygous mother plant (M/W) by genotyping. Plant growth assay and neighborhood experiments were performed to quantify variation between M/M plants and W/W plants. The Ile1781Leu mutation resulted in slight increases in plant growth in pure stands and improved resource competitiveness under low‐competition conditions in pot experiments, but no clear variation was observed under high competitive pressure or field conditions. During competition with wheat plants under field conditions, American sloughgrass plants containing Ile2041Asn ACCase exhibited a significantly lower (12.5%) aboveground biomass but no distinct differences in seed production or resource competitiveness. No significant detrimental pleiotropic effects associated with Gly2096Ala were detected in American sloughgrass. The Trp2027Cys mutation distinctly reduced seed production, especially under high competitive pressure, but did not significantly alter plant growth. The Asp2078Gly mutation consistently reduced not only plant growth and seed production but also resource competitiveness. Synthesis . The Trp2027Cys and Asp2078Gly mutations led to significant fitness costs, which may reduce the frequency of resistance alleles and reduce the propagation speed of resistant weeds in the absence of ACCase inhibitor herbicides. The Ile1781Leu, Ile2041Asn, and Gly2096Ala mutations displayed no obvious fitness costs or displayed very small fitness penalties, which would likely have no effect on the establishment of resistant weeds in the field.

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Identification and expression of main genes involved in non‐target site resistance mechanisms to fenoxaprop‐p‐ethyl in Beckmannia syzigachne

Bai

Zhao

Zhou

et al. 2020

Pest Management Science

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BACKGROUNDNon‐target‐site resistance (NTSR) to herbicides is a serious threat to global agriculture. Although metabolic resistance is the dominant mechanism of NTSR, the molecular mechanisms are not yet well‐characterized. This study aimed to uncover the likely metabolism‐related genes in Beckmannia syzigachne (American sloughgrass) resistant to fenoxaprop‐p‐ethyl.RESULTSUltra‐performance liquid chromatography – tandem mass spectrometry experiments showed that the resistant American sloughgrass biotype (R, SD‐04‐SS) showed enhanced degradation of this herbicide compared to the susceptible biotype (S, SD‐12). R and S biotype were harvested at 24 h after fenoxaprop‐p‐ethyl treatment to conduct RNA sequencing (RNA‐Seq) analysis to investigate the likely fenoxaprop‐p‐ethyl metabolic genes. The RNA‐Seq libraries yield 417 969 980 clean reads. The de novo assembly generated 115 112 unigenes, of which 57 906 unigenes were annotated. Finally, we identified 273 cytochrome P450s, 178 oxidases, 47 glutathione S‐transferases (GSTs), 166 glucosyltransferases (GTs) and 180 ABC transporter genes to determine the likely fenoxaprop‐p‐ethyl metabolism‐related genes in R biotype. Twelve overlapping up‐regulated genes in the R biotype (fenoxaprop‐p‐ethyl‐treated R/non‐treated R, fenoxaprop‐p‐ethyl‐treated R/fenoxaprop‐p‐ethyl‐treated S) were identified by RNA‐Seq and the results were validated using qRT‐PCR. Ten were identified as fenoxaprop‐p‐ethyl metabolism‐related genes, including three P450s (homologous to CYP71D7, CYP99A2 and CYP71D10), one GST (homologous to GSTF1), two GTs (homologous to UGT90A1 and UGT83A1) and four oxidase genes.CONCLUSIONThis work demonstrates that the NTSR mechanism by means of enhanced detoxification of fenoxaprop‐p‐ethyl in American sloughgrass is very likely driven by herbicide metabolism related genes. The RNA‐Seq data presented here provide a valuable resource for understanding the molecular mechanism of NTSR in American sloughgrass. © 2020 Society of Chemical Industry

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Cross-resistance patterns to ACCase-inhibitors in American sloughgrass (Beckmannia syzigachne Steud.) homozygous for specific ACCase mutations

Cited by 24 publications

References 35 publications

Effect of herbicide resistance endowing three ACCase mutations on seed germination and viability in American slough grass ( Beckmannia syzigachne Steud. Fernald)

Effect of herbicide resistance endowing three ACCase mutations on seed germination and viability in American slough grass ( Beckmannia syzigachne Steud. Fernald)

Fitness costs associated with acetyl‐coenzyme A carboxylase mutations endowing herbicide resistance in American sloughgrass (Beckmannia syzigachne Steud.)

Identification and expression of main genes involved in non‐target site resistance mechanisms to fenoxaprop‐p‐ethyl in Beckmannia syzigachne

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