Cross‐reactivity of Olea europaea with other Oleaceae species in allergic rhinitis and bronchial asthma

Abstract

Cross-reactivity between pollen extracts of four species of Oleaceae was studied: olive (Olea europaea), ash (Fraxinus excelsior), privet (Ligustrum vulgare), and lilac (Syringa vulgaris). Thus, 51 patients and 13 atopic controls were studied, by means of intracutaneous skin tests, histamine-release tests against the four extracts, and specific IgE to O. europaea. The proteic content of the four extracts was assessed by SDS-PAGE and immunoblotting, and similarity of all the extracts studied was observed after electrophoresis and immunodetection. Six common bands were found to be responsible for the cross-reactivity, with apparent molecular weights of 49.6, 40, 36.7, 19.7, 16.7, and 14 kDa, respectively. The cross-reactivity was also corroborated by immunoblotting inhibition and FEIA inhibition. The patients had a similar response to the four allergenic extracts used, although the response to Olea was greatest. When the patients were compared by their geographic origin (northern or southern Spain, according to the distribution of areas of olive pollen influence), there were no significant differences between the two groups in skin reactivity, but a higher histamine release was observed for the four extracts in the southern group, although it was significant only for Fraxinus and Ligustrum. This work corroborated the practicality of the diagnostic methods used and the cross-reactivity between the four species studied, as demonstrated by the different methods used. Therefore, we suggest that only O. europaea extract be used in diagnosis and immunotherapy in Oleaceae pollen allergy.

NMR Solution Structure of Ole e 6, a Major Allergen from Olive Tree Pollen

“…Many side chain conformations of residues in rOle e 6 are well defined, with 22 residues (5,8,9,10,11,12,13,15,16,17,18,19,21,24,25,26,27,29,30,31,32, and 33) having 1 circular variances lower than 0.05. These residues with well defined side chain conformations mainly correspond to residues exhibiting high values of surface inaccessibility and to residues located in the secondary structural elements.…”

“…Important cross-reactions with other Oleaceae pollens, like ash (Fraxinus excelsior), lilac (Syringa vulgaris), or privet (Ligustrum vulgare), have been described previously (13)(14)(15)(16)(17)(18)(19). At the moment, 10 allergens from olive pollen, Ole e 1-10, have been described (20 -24), but their three-dimensional structures have not been determined.…”

Hypersensitivity to Pollen of Olea Europea in Patients with Pollen Allergy in Zadar County, Croatia

“…To znači da pojedinci mogu pokazivati preosjetljivost ograničenu na pelud pojedine biljne vrste, ali i na pelude biljnih vrsta iz istoga roda, koje imaju strukturno vrlo slične antigene i dovode do ukriženih imunosnih reakcija (21). Ubodnim kožnim testom kod 5 % bolesnika u našem istraživanju dokazana je ukrižena reaktivnost u kožnom testu na maslinu (Olea europea) i jasen (Fraxinus ornus).…”

“…Unatoč tomu ni jedan od navedenih bolesnika u serumu nije imao povišene vrijednosti specifi čnih IgE na Fraxinus ornus. I drugi autori (21) koji su proveli slična ispitivanja pokazali su da je ukrižena reaktivnost u kožnom testu moguća među pojedinim kulturama porodice Oleacea, a osobito je česta ukrižena reaktivnost na Fraxinus ornus. Unatoč tomu imunosni odgovor senzibilizirane osobe ipak je izraženiji na antigen masline (Olea europea) u usporedbi s drugim kulturama iz ove porodice (21).…”

Standardization of an Ash <i>(Fraxinus excelsior) </i>Pollen Allergen Extract

“…The differential diagnosis of ash and birch pollen allergies based on total extracts is difficult because of immune cross-reactivity between these two pollens. Previous studies have described the existence of immunologic cross-reactivity among Oleaceae members due to their phylogenic proximity [7,14,15,16,17,18]. Currently available in vivo or in vitro diagnostic reagents cannot distinguish cosensitization to the two pollens from cross-sensitization due to minor allergens.…”