Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus

Pipattanaboon, Chonlatip; Sasaki, Tadahiro; Nishimura, Mitsuhiro; Setthapramote, Chayanee; Pitaksajjakul, Pannamthip; Leaungwutiwong, Pornsawan; Limkittikul, Kriengsak; Puiprom, Orapim; Sasayama, Mikiko; Chaichana, Panjaporn; Okabayashi, Tamaki; Kurosu, Takeshi; Ono, Kenichiro; Ramasoota, Pongrama; Ikuta, Kazuyoshi

doi:10.2147/btt.s47438

Cited by 10 publications

(8 citation statements)

References 39 publications

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“…Flavivirus antibodies are known to be highly cross reactive in nature and are known to give false positive responses in antibody detection assays [ 16 , 19 , 20 ] and the higher JEV seropositivity in adults who were hospitalized due to dengue, could possibly be due to higher cross-reactive DENV antibody titres (and therefore JEV antibody titres). Therefore, we determined the association between the optic density (OD) values for DENV and JEV in those with hospitalized dengue and those who had asymptomatic/mild dengue.…”

Section: Resultsmentioning

confidence: 99%

Change in Dengue and Japanese Encephalitis Seroprevalence Rates in Sri Lanka

et al. 2015

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BackgroundSri Lanka has been affected by epidemics of dengue infections for many decades and the incidence and severity of dengue infections have been rising each year. Therefore, we investigated the age stratified seroprevalence of dengue infections in order to facilitate future dengue vaccine strategies. In addition, since the symptomatic dengue infections have increased during the past few decades, we also investigated the possible association with Japanese Encephalitis Virus (JEV) antibody seropositivity with symptomatic dengue in a community cohort in Sri Lanka.Methods1689 healthy individuals who were attending a primary health care facility were recruited. Dengue and JEV antibody status was determined in all individuals and JEV vaccination status was recorded.Results1152/1689 (68.2%) individuals were seropositive for dengue and only 133/1152 (11.5%) of them had been hospitalized to due to dengue. A significant and positive correlation was observed for dengue antibody seropositivity and age in children (Spearmans R = 0.84, p = 0.002) and in adults (Spearmans R = 0.96, p = 0.004). We observed a significant rise in the age stratified seroprevalence rates in children over a period of 12 years. For instance, in year 2003 the annual seroconversion rate was 1.5% per annum, which had risen to 3.79% per annum by 2014. We also found that both adults (p<0.001) and in children (p = 0.03) who were hospitalized due to dengue were more likely to be seropositive for JEV antibodies. However, 244 (91.4%) of adults who were seropositive for JEV had not had the JEV vaccine.ConclusionsDengue seroprevalence rates have risen significantly over the last 12 years in Sri Lanka, possibly due to increased transmission. As individuals who were hospitalized due to dengue were more likely to be seropositive for JEV, the possibility of cross-reactive assays and/or of JEV infection on immunity to the DENV and clinical disease severity should be further investigated.

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Section: Resultsmentioning

confidence: 99%

Change in Dengue and Japanese Encephalitis Seroprevalence Rates in Sri Lanka

et al. 2015

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“…Furthermore, the detection of the presence of NS1 in the blood is one of the dengue diagnostic methods [ 2 ]. However, the sensitivity of NS1 RDT was ranging from 44.4% to 87% [ 25 – 28 ] in the detection of DENV when compared to real-time RT-PCR. Besides, the circulating level of NS1 in asymptomatic DENV infections has not been determined.…”

Section: Discussionmentioning

confidence: 99%

Low frequency of asymptomatic dengue virus-infected donors in blood donor centers during the largest dengue outbreak in Taiwan

et al. 2018

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To determine the prevalence of asymptomatic dengue virus-infected blood donors during the largest dengue outbreak in Taiwan history occurred in 2015, we examined the evidence of dengue virus (DENV) infection by the detection of DENV RNA genome using real-time reverse transcription-polymerase chain reaction (real-time RT-PCR), DENV NS1 antigen using rapid diagnosis test (RDT) and anti-dengue antibody using IgM/IgG capture enzyme-linked immunosorbent assay (capture ELISA) and RDT in eight thousand serum samples from blood donations to the blood centers of the Taiwan Blood Services Foundation (TBSF) in Kaohsiung City and Tainan City during the largest dengue outbreak in Taiwan history occurred in 2015. Only one serum sample was positive for DENV RNA detection by using dengue-specific real-time RT-PCR, the virus was DENV-2 determined by serotype-specific real-time RT-PCR and sequencing, and the DENVs in the serum were confirmed as being infectious by a plaque assay. The recipient of this blood did not develop any dengue fever symptom on follow-up. None of the samples was NS1 RDT-reactive. Seventeen IgM-positive samples were identified. There was a low prevalence of asymptomatic confirmed or probable DENV-infected blood donors in our study (0.013% and 0.21%, respectively), and no symptomatic transfusion-transmitted dengue (TT dengue) was developed during the largest dengue outbreak in Taiwan history in highly endemic areas and periods.

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“…Previous studies have found different sensitivities for the SD NS1 RDT, ranging from 44.4% to 87% [ 13 , 20 – 23 ]. The sensitivity of the CTK NS1 RDT reported by a previous study was 40% [ 24 ], and it has been reported that the RDTs show higher sensitivity with primary infection than with secondary infection samples [ 13 , 24 – 26 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of two rapid diagnostic tests during a large dengue virus serotype 3 outbreak in the Solomon Islands in 2013

Liu

Dalipanda²,

Jagilly³

et al. 2018

PLoS ONE

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Dengue virus (DENV) infection causes various clinical presentations, including asymptomatic infection, dengue with or without warning signs and severe dengue. An early and accurate diagnosis of DENV infection during the first few days of illness supports clinical management and significantly reduces dengue-associated mortality and morbidity. However, it is very difficult to confirm DENV infection in endemic regions without qualified dengue diagnostic laboratories. In this study, we evaluated the performance of two commercially available rapid diagnostic tests (RDTs) using serum samples collected in the Solomon Islands during the 2013 DENV-3 outbreak. The sensitivity and specificity of the tests were calculated by comparing the results of DENV nonstructural protein 1 (NS1), IgM and IgG RDTs with those obtained by qRT-PCR. We also compared the results of the DENV IgM/IgG RDT with those obtained using an IgM/IgG capture enzyme-linked immune-sorbent assay (ELISA). The sensitivities of the SD and CTK NS1 RDTs were similar (90.9% and 92.6%), and the specificity of the SD NS1 RDT was significantly higher than that of the CTK NS1 RDT (100% versus 78.8%). The inclusion of IgM and IgG in the RDT did not significantly increase the sensitivity for DENV diagnosis. Compared with the SD IgM RDT, IgM capture ELISA had the same specificity but higher sensitivity. User-friendly RDTs remain the first choice and the most convenient tool in dengue endemic regions, where laboratory facilities and the corresponding infrastructure are lacking. Our study provided important and practical information for comparing the performance and validity of the different RDTs for rapid dengue detection.

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Cross-reactivity of human monoclonal antibodies generated with peripheral blood lymphocytes from dengue patients with Japanese encephalitis virus

Cited by 10 publications

References 39 publications

Change in Dengue and Japanese Encephalitis Seroprevalence Rates in Sri Lanka

Change in Dengue and Japanese Encephalitis Seroprevalence Rates in Sri Lanka

Low frequency of asymptomatic dengue virus-infected donors in blood donor centers during the largest dengue outbreak in Taiwan

Comparison of two rapid diagnostic tests during a large dengue virus serotype 3 outbreak in the Solomon Islands in 2013

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