Cross-reactivity of Antibody to Human and Bovine Luteinizing Hormone (LH) with Homologous and Heterologous LH<sup>1</sup>

Reichert, Leo E.; Treadwell, Perry E.

doi:10.1210/endo-81-3-591

Cited by 11 publications

(5 citation statements)

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“…These results provide striking evidence for the nonidentity of sites for immunologic and biologic activity in the rat LH molecule. Similar results have been obtained with ovine (Reichert and Treadwell, 1967) and human LH (Reichert, 1969;Reichert and Midgley, 1968b); also see pertinent abstracts by Parlow (1970) and Papkoff etal. (1970).…”

Section: Discussionsupporting

confidence: 77%

Isolation and properties of subunits of rat pituitary luteinizing hormone

Ward¹,

Reichart²,

Fitak³

et al. 1971

Biochemistry

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Section: Discussionsupporting

confidence: 77%

Isolation and properties of subunits of rat pituitary luteinizing hormone

Ward¹,

Reichart²,

Fitak³

et al. 1971

Biochemistry

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“…The NIH-TSH-S5 used in this study was treated with hydrogen peroxide to destroy the biological activity of LH present in the preparation ( 11). However, recently Reichert and Treadwell (12) reported that ovine LH treated in the same way retained its ability to react immunologically with antibovine LH * NIH-LH-S14.…”

Section: Storedmentioning

confidence: 98%

Serum Levels of Prolactin and Luteinizing Hormone (LH) in the Ewe at Various Stages of the Estrous Cycle

Reeves¹,

Arimura²

1970

Experimental Biology and Medicine

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“…pg of NIH-LH-S1(12).HCG were assayed for capacitation in superovulated test rabbits inseminated either within the uterus or within the oviduct 12.5 hr after an injection of ovulating hormone. Injection of LH at levels up to 2000 Jug had no significant influence on sperm capacitation, but 300 IU of HCG reduced fertility.…”

mentioning

confidence: 99%

Sperm Capacitation after Injection of LH or HCG in Rabbits

Wettemann

Hafs

1970

Experimental Biology and Medicine

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Sperm capacitation (1,2) in the rabbit uterus occurs more rapidly when estrogen is the predominant gonadal hormone (3). Soupart (4, 5) reported that sperm capacitation proceeds more rapidly after mating than after surgical insemination into the uterine lumen, and that an injection of 75 IU of HCG enhances sperm capacitation similarly to mating in rabbits. These observations prompted us to compare the influence of HCG with that of LH on sperm capacitation.Materials and Methods. Mature estrous rabbits of mixed breeding and weighing 3 to 5 kg were used for capacitator (6) and test rabbits. Each rabbit was caged individually for at least 1 month a t 18' with a 14-hr photoperiod before assignment to an experimen t.In any experiment, semen samples were pooled from 2 to 4 rabbits with proven fertility. Seminal plasma was discarded and the sperm were resuspended to 500 million/ml of Locke's solution with 100 pg of dihydrostreptomycin sulfate /ml. Under ether anesthesia, the uterine horns of capacitator rabbits were exposed via a midventral incision and 90 to 200 million sperm (at least 50% motile) were injected within each uterine horn near the tubouterine junction with a 26-gauge needle.

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Cross-reactivity of Antibody to Human and Bovine Luteinizing Hormone (LH) with Homologous and Heterologous LH¹

Cited by 11 publications

References 0 publications

Isolation and properties of subunits of rat pituitary luteinizing hormone

Isolation and properties of subunits of rat pituitary luteinizing hormone

Serum Levels of Prolactin and Luteinizing Hormone (LH) in the Ewe at Various Stages of the Estrous Cycle

Sperm Capacitation after Injection of LH or HCG in Rabbits

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Cross-reactivity of Antibody to Human and Bovine Luteinizing Hormone (LH) with Homologous and Heterologous LH1

Cited by 11 publications

References 0 publications

Isolation and properties of subunits of rat pituitary luteinizing hormone

Isolation and properties of subunits of rat pituitary luteinizing hormone

Serum Levels of Prolactin and Luteinizing Hormone (LH) in the Ewe at Various Stages of the Estrous Cycle

Sperm Capacitation after Injection of LH or HCG in Rabbits

Contact Info

Product

Resources

About

Cross-reactivity of Antibody to Human and Bovine Luteinizing Hormone (LH) with Homologous and Heterologous LH¹