Cross Reactivity between EBV and Herpes Simplex Virus

Balachandran, N.; Oba, D E; Hutt-Fletcher, Lindsey

doi:10.1007/978-1-4612-4590-2_59

Epstein-Barr Virus and Human Disease 1987

DOI: 10.1007/978-1-4612-4590-2_59

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Cross Reactivity between EBV and Herpes Simplex Virus

N. Balachandran

D E Oba

Lindsey Hutt-Fletcher

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1995

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“…Fixed P3HR1 cells were used to demonstrate that gp85 MAbs F-2-1 (Strnad et al, 1982) and E1D1 (Balachandran et al, 1987), affinity-purified with Protein-G, exhibited strong immunofluorescence with the P3HR1 EBV-transformed human B cell line ( Fig. 1 a, b) demonstrating their suitability as reagents for the detection of native gp85.…”

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confidence: 99%

Co-expression of the Epstein--Barr virus BXLF2 and BKRF2 genes with a recombinant baculovirus produces gp85 on the cell surface with antigenic similarity to the native protein

Pulford

Lowrey²,

Morgan³

1995

Journal of General Virology

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Glycoprotein H (gH) is a conserved herpesvirus gene product functionally implicated in the penetration of the virus into the host cell. Other human herpesviruses require an accessory glycoprotein named gL for the synthesis of mature gH. We constructed a series of recombinant baculoviruses to determine whether gL expression can overcome the constraints upon Epstein-Barr virus (EBV) gH (gp85) folding and transport in this expression system. When gH and gL were co-expressed some EBV gH was transported to the insect cell surface. Deletion of 27 amino acids from the gH carboxy terminus resulted in the secretion of an 80 kDa protein (gHt) into the culture medium when it was expressed either in the presence or absence of gL and this protein could also be immunoprecipitated with E1D1. In contrast, gL was not secreted into the culture medium. Our results suggests that either co-expression of gH with gL or removal of the predicted transmembrane anchor sequence can overcome some of the constraints upon EBV gH expression in the baculovirus system.

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mentioning

confidence: 99%

Co-expression of the Epstein--Barr virus BXLF2 and BKRF2 genes with a recombinant baculovirus produces gp85 on the cell surface with antigenic similarity to the native protein

Pulford

Lowrey²,

Morgan³

1995

Journal of General Virology

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Cross Reactivity between EBV and Herpes Simplex Virus

Cited by 1 publication

References 2 publications

Co-expression of the Epstein--Barr virus BXLF2 and BKRF2 genes with a recombinant baculovirus produces gp85 on the cell surface with antigenic similarity to the native protein

Co-expression of the Epstein--Barr virus BXLF2 and BKRF2 genes with a recombinant baculovirus produces gp85 on the cell surface with antigenic similarity to the native protein

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